TNF Recombinant Human Protein

Catalog number: PHC3016

Gibco™  Related applications: Cell Viability, Proliferation & Function | Mammalian Cell Culture

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Description

Recombinant Human Tumor Necrosis Factor-α (TNF) is a bioactive protein intended for use in cell culture applications. TNF causes cytolysis and cytostasis of many tumor cell lines. TNF has a wide spectrum of activities, including chemotaxis of neutrophils, alteration of the endothelium, inhibition of anticoagulatory mechanisms, and promotion of angiogenesis.

High purity—no interference from other proteins or contaminants
High biological activity—more results with less protein
Proven compatibility—Gibco® proteins bioassayed with Gibco® media

High Purity Recombinant Bioactive Protein
Recombinant Human Tumor Necrosis Factor-α is one of our more than 250 available Gibco® recombinant proteins. To help ensure Gibco® growth factors are of the highest quality, each protein is analyzed for purity along with structural homogeneity to ensure a biologically active protein.

High Biological Activity
In-house activity testing of Gibco® Recombinant Proteins includes cell proliferation, cytotoxicity, chemotaxis, calcium flux, secondary cytokine up-regulation, induction of surface antigen expression, antiviral, and protease assays. Recombinant Human Tumor Necrosis Factor-α (TNF) specific activity is determined by the dose dependent cytotoxic effect on L929 cells in the presence of actinomycin D.

Proven Compatibility
Gibco® proteins are bioassayed with Gibco® media. Since 1962, Gibco® has been the consistent provider of high quality media, reagents, and sera for reliable cell culture.

See our selection of recombinant Gibco® growth factors.

For Research Use Only. Not intended for animal or human diagnostic or therapeutic use.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Lyophilized
Mass: 50 µg
Purity: 95 %
Gene ID: 7124
Species: Human
Expression: E. coli
Gene Aliases: TNF-α
Product Size: 50 µg
Protein Form: Recombinant, Ligand
Purification: Sequential Chromatography
Protein Family: Cytokines & Receptors
Endotoxin Level: < 0.1 ng/µg
Protein Subtype: TNF (Tumor Necrosis Factor)
Molecular Weight: 17.5 kDa
Research Category: Immunology, Stem Cell Research, Inflammation, Signal Transduction, Oncology
Validated Application: Cell Culture
Purity or Quality Grade: Carrier-Free
Shipping Condition: Wet Ice

Contents & storage

Store in refrigerator (2–8°C).

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Manuals & protocols

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