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Qdot® Streptavidin Sampler Kit

Catalog number: Q10151MP

Novex™  Related applications: Cellular Imaging | Labeling Chemistry

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Description

Qdot® streptavidin conjugates consist of a biotin-binding protein (streptavidin) covalently attached to a fluorescent label (Qdot® nanocrystal). Streptavidin has a very high binding affinity for biotin, and a conjugate of streptavidin is commonly used together with a conjugate of biotin for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules (for example, a biotinylated primary antibody bound to a protein target can be detected with a fluorescently labeled streptavidin). Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays, and are also used in purification workflows to achieve targeted fractionation. Each of the six conjugates in the Qdot® Streptavidin Sampler Kit is supplied as a 1 µM solution (50 µL volume).

Important Features of the Qdot® Streptavidin Sampler Kit:
Streptavidin Qdot® conjugates have emission maxima of ~525, ~565, ~585, ~605, ~655, and ~705 nm
Approximately 5 to 10 streptavidins per Qdot® nanocrystal
Extremely photostable and bright fluorescence
Efficiently excited with single-line excitation sources
Narrow emission, large Stokes shift
Available in multiple colors
Ideal for western blots, flow cytometry, imaging and microscopy, microplate assays and more

Properties of Qdot® Nanocrystals
The Qdot® streptavidin conjugate is the size of a large macromolecule or protein (~15–20 nm) and represents the brightest class of streptavidin detection reagents. Qdot® streptavidin conjugates are made from a nanometer-scale crystal of a semiconductor material (CdSe), which is coated with an additional semiconductor shell (ZnS) to improve the optical properties of the material. The Qdot® 705 and Qdot® 800 streptavidin conjugates, which include CdSeTe, are made in a similar fashion. This core-shell material is further coated with a polymer shell that allows the materials to be conjugated to biological molecules and to retain their optical properties.

Other Fluorescent Conjugates of Streptavidin are Available
We offer several other Qdot® colors and wide range of streptavidins conjugated to Alexa Fluor® dyes, Oregon Green® dye, enzyme conjugates, and traditional fluorophores like Texas Red® dye, fluorescein (FITC) and more.

Find Biotinylated Conjugates
We offer an extensive line of biotinylated conjugates for use in biotin-streptavidin detection strategies.
• Use the primary antibody search tool to find biotinylated primary antibodies
• Use the secondary antibody selector tool to find biotinylated secondary antibodies and biotinylated anti-dye and anti-hapten antibodies

Blocking Endogenous Biotin
Naturally occurring biotins can interfere with biotin-streptavidin detection schemes. For experiments involving fixed and permeabilized cells, try our Endogenous Biotin-Blocking Kit to minimize this interference.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Links:

Learn more about Avidin-Biotin Detection

To learn more about Qdot® Nanocrystals
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Qdot® 655, Qdot® 605, Qdot® 525, Qdot® 565, Qdot® 585, Qdot® 705
Product Size: 1 kit
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C).

Documents

Manuals & protocols

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