Qubit® Protein Assay Kit

Catalog number: Q33211

Molecular Probes™  Related applications: Protein Assays and Analysis

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Description

The Qubit® (previously known as Quant-iT™) Protein Assay Kit may be used with any fluorometer or fluorescence plate reader. Using between 1 and 20 µl of your sample, this assay can quantitate samples ranging from 12.5 µg⁄ml to 5 mg⁄ml and exhibits low protein to protein variation. The assay is highly selective for proteins and is designed to be accurate in the presence of reducing reagents, but not in the presence of a large amount of detergent. Common contaminants, such as reducing reagents (DTT, β-mercaptoethanol), salts, free nucleotides, amino acids, solvents, or DNA, but not detergents, are well tolerated in the assay. Slight protocol modifications are required for other contaminants. The kit provides concentrated assay reagent, dilution buffer, and pre-diluted BSA standards. Simply dilute the reagent using the buffer provided, add your sample (any volume between 1 µL and 20 µL is acceptable), and read the concentration.

Notes:
1. All Qubit® assay kits can also be used with the Qubit® 1.0 Fluorometer.
2. 500 µL thin-walled PCR tubes are required but not included.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Assay Type: Protein Quantitation Assay
Product Size: 100 assays
Detection Method: Fluorescent
Quantitation Range: 0.25-5 µg
Number of Reactions: 100 Reactions
For Use With (Equipment): Microplate Reader, Qubit® Fluorometer
Shipping Condition: Room Temperature

Contents & storage

Store in refrigerator (2–8°C) and protect from light.

Note: 500 µL thin-walled PCR tubes are required but not included.

Documents

Manuals & protocols

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