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ActinGreen™ 488 ReadyProbes® Reagent

Catalog number: R37110

Molecular Probes™  Related applications: Cell Structure

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Description

ActinGreen™ 488 ReadyProbes® Reagent is a selective, high-affinity F-actin probe conjugated to our bright, photostable, green-fluorescent Alexa Fluor® 488 dye. Like other ReadyProbes® reagents, the ActinGreen™ 488 reagent is formulated as a room temperature-stable solution and comes in a convenient dropper bottle. Just tip and drip two drops per ml to stain your cells.

• High-affinity staining of F-actin with superior specificity compared to antibody methods
• Outstanding fluorescence performance (Ex/Em: 495/518 nm)
• Ready-to-use liquid formulation in convenient dropper bottle—no need to dilute, weigh, or pipette
• Stability at room temperature—keep handy at your scope or cell culture area

See other ReadyProbes® reagents for cell staining
Learn more about other stains for actin

Cell imaging applications
Phalloidin is a bi-cyclic peptide, commonly used in imaging applications to selectively label F-actin. Fluorescently-labeled phalloidin has several advantages over antibodies for actin labeling, including virtually identical binding properties with actin from different species of plants and animals, and low non-specific binding. Phalloidin binds F-actin with high selectivity, while Alexa Fluor® 488 dye provides green fluorescence of unparalleled brightness and photostability.

Suggestions for use
• ActinGreen™ 488 reagent may be added directly to fixed cells in full media or buffer solutions.
• In most cases, 2 drops/ml and an incubation time of 15 to 30 minutes is sufficient for bright actin staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more or fewer drops until the optimal staining intensity is obtained.
• ActinGreen™ 488 dye is excited with a maximum at 495 nm and has an emission maximum at 518 nm. It is detected through standard GFP and FITC filters.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Label or Dye: Alexa Fluor™ 488
Sub-Cellular Localization: Cytoskeleton
Form: Liquid
Color: Green
Product Size: 1 kit
Emission Class: Visible
Detection Method: Fluorescent
Excitation Class: Visible
For Use With (Equipment): Fluorescence Microscope, Floid® Cell Imaging System, Flow Cytometer, Confocal Microscope
Excitation⁄Emission (nm): 495⁄518

Contents & storage

2 × 2.5 mL dropper bottles

Store at ≤ 25°C.

Documents

Manuals & protocols

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