Use ProLong® Live Antifade Reagent for brightest fluorescent images with live cells

BackDrop® Background Suppressor ReadyProbes® Reagent

Catalog number: R37603

Molecular Probes™  Related applications: Cellular Imaging

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Description

BackDrop® Background Suppressor ReadyProbes® Reagent is a set of novel reagents designed to effectively suppress background fluorescence in live cell imaging samples. BackDrop® Background Suppressor is a direct addition product that comes in ultra-convenient dropper bottles as a six-pack with two vials each of blue, green, and red background suppressors. Apply two drops per ml and watch background disappear.

• Higher signal-to-noise, improved sensitivity, and better results
• Convenience of direct addition to cells from dropper bottle
• Flexibility to modulate blue, green, or red background signal

See other ReadyProbes® reagents for cell staining
Find more tools for fixed cell imaging

Reduce background signal in live cells without removing media
Background signals from media components, non-specific labeling, or fluorophores in solution decrease signal-to-noise ratio and thus reduce assay sensitivity. The degree of background suppression varies with fluorophore, concentration, labeling efficiency, cells, and other conditions, but can often be significant (Figure 1). Add a few drops of BackDrop® Background Suppressor to your live cell imaging sample, eliminate background haze, improve image quality, and eliminate the need for medium removal and potential cell loss. BackDrop® Background Suppressor will allow you to either view cells without having to wash off unloaded dye, or to determine when cell loading is optimal.

Suppress Blue, Green, and Red Background
The blue, green, and red background suppressors are compatible with the Hoechst, FITC, and Texas Red filters, respectively. Choose the BackDrop® color where background is a problem, or combine all three. BackDrop® Background Suppressor is most effective if cells are photographed within one hour of application, though longer treatment will not harm cells.

Convenient and Easy to Use
BackDrop® Background Suppressor has been formulated for stability at room temperature. Simply keep the convenient box and dropper bottles by your imager or microscope and add two drops per ml whenever you are ready to label and view your cells—in high contrast.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Reagent Type: Blocking⁄Background Suppression Reagent
Product Size: 1 kit
Shipping Condition: Room Temperature

Contents & storage

2 x 2.5 mL BackDrop Red Background Suppressor
2 x 2.5 mL BackDrop Green Background Suppressor
2 x 2.5 mL BackDrop Blue Background Suppressor

Store at ≤ 25°C.

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