Use ProLong® Live Antifade Reagent for brightest fluorescent images with live cells

SYTOX® Green Nucleic Acid Stain - 5 mM Solution in DMSO

Catalog number: S7020

Molecular Probes™  Related applications: Cell Structure | Cell Tracing & Tracking | Cell Viability, Proliferation & Function | Cellular Imaging | Flow Cytometry

Error loading your content!

  Catalog number
Select a plan
Unit size
List price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

SYTOX® Green nucleic acid stain is an excellent green-fluorescent nuclear and chromosome counterstain that is impermeant to live cells, making it a useful indicator of dead cells within a population.
 Impermeant to live cells
 Excitation/Emission: 504/523 nm
 Use with 488 Argon-ion laser
 Works with mammalian cells and both Gram-positive and Gram-negative bacteria

Simple Viability Determination
SYTOX® Green allows quick determination of cell viability when using flow cytometers, fluorescence microscopes, fluorometers, or fluorescence microplate readers. It will not cross intact membranes, but will easily penetrate compromised membranes characteristic of dead cells. Because it exhibits >500-fold fluorescence enhancement upon binding nucleic acids, briefly incubating dead cells with SYTOX® Green will result in bright green fluorescence with an emission peak of 523 nm when excited by a 488 nm argon-ion laser or any other 450–490 nm source. The exceptional brightness of the signal produced in dead cells makes SYTOX® Green particularly useful for determining viability of not just mammalian cells, but both Gram-positive and Gram-negative bacteria.

Part of the Invitrogen Family of Cell Viability Stains and Assays
SYTOX® stains for dead cells are available in a variety of colors, including red, blue, and orange. Additionally, Invitrogen has incorporated SYTOX® stains into a number of assays for apoptosis, cell viability, and metabolism.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

Related Link
•  Find out more about all SYTOX® Products.

For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

For Use With (Equipment): Flow Cytometer
Cell Permeability: Cell-Impermeant
Sub-Cellular Localization: Nucleus, Nucleic Acids
Form: Solution
Format: Tube(s)
Label or Dye: SYTOX® Green
Product Size: 250 µL
Detection Method: Fluorescent
Flow Cytometer Laser Lines: 488
Excitation⁄Emission (nm): 504⁄523
Shipping Condition: Room Temperature

Contents & storage

Contains 1 vial of SYTOX® green nucleic acid stain (5 mM solution in DMSO).

Store in freezer (-5 to -30°C) and protect from light.

Documents

Manuals & protocols

Recommended products