Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System

Catalog number: T1009

Novex™  Related applications: Protein Assays and Analysis

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Description

The Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of β-galactosidase in reporter gene assays.

• The Galacto-Light Plus™ β-Galactosidase Reporter Gene Assay System is designed for luminometers without injectors.
• Wide dynamic range of β-galactosidase assay lets the user measure enzyme level accurately from femtogram to nanogram range.
• Assay sensitivity is 100- to 1,000-fold better than with either the isotopic⁄non-isotopic assays for chloramphenicol acetyl transferase (CAT) or the colorimetric⁄fluorescent assays for β-galactosidase, providing greater sensitivity than competing assays.
• Highly sensitive assay with a wide dynamic range permits detection of high and low levels of reporter without performing numerous sample dilutions.
• Non-radioactive reporter gene assay kit eliminates concerns over use of radioisotopes.
• Assay can be completed in about one hour, providing fast assay turnaround.

Sensitive
This chemiluminescent assay exhibits over three orders of magnitude greater sensitivity compared to colorimetric β-galactosidase assays and can be completed in a fraction of the time required for assays for chloramphenicol acetyltransferase (CAT) and ELISA-based reporter assays.

Ready-to-Use
The Galacto-Light™ Plus™ system incorporates chemiluminescent substrates, reaction buffer, and light-emission accelerator containing luminescence enhancers, in a ready-to-use format. The reaction buffer is supplied at a pH that aids in discrimination of endogenous β-galactosidase, while having little effect on the bacterial form of the enzyme. This enables sensitive detection even in cell lines with relatively high levels of endogenous β-galactosidase activity.

Products described herein are guaranteed for 12 months from the date of purchase.

For Research Use Only. Not for use in diagnostics procedures.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Liquid
Format: 96-well plate
Readout: End Point
Substrate: Galacton-Plus®
Technique: Enhanced Chemiluminescence
Assay Type: β-Gal (lacZ) Assay
Product Size: 15,000 assays
Target Enzyme: Beta-Galactosidase
Substrate Type: Beta-Gal Substrate
Compatible Cells: Mammalian Cells
Detection Method: Chemiluminescent
Substrate Properties: Chemical Substrate
Sample Type (Specific): Cell Cultures
For Use With (Equipment): Luminometer (Microplate)
High Throughput Compatibility: High Throughput-Compatible

Contents & storage

This kit contains the lysis solution, substrate, reaction buffer, and accelerator.
Store at 4°C.

Documents

Manuals & protocols