Invitrogen™

      pPIC9K Pichia Vector

      Catalog number:  V17520

       Related applications: Protein Expression

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      Description

      The pPIC9K vector carries the kanamycin resistance gene that confers resistance to Geneticin® Reagent in Pichia. Spontaneous generation of multiple insertion events can be identified by resistance to increased levels of Geneticin® Reagent. Pichia transformants are selected on histidine-deficient medium and screened for their level of resistance to Geneticin® Reagent. The ability to grow in high concentrations of Geneticin® indicates that multiple copies of the kanamycin resistance gene and the gene of interest are integrated into the genome. The pPIC9K vector directs secretion of expressed proteins. This vector is included in the Multi-Copy Pichia Expression Kit.
      For Research Use Only. Not for use in diagnostic procedures.

      Specifications

      Antibiotic Resistance (Bacterial): Ampicillin (AmpR), Gentamicin (GmR)
      Cloning Method: Restriction Enzyme ⁄ MCS
      Product Size: 20 µg
      Promoter: AOX1
      Protein Tag or Fusion: Untagged
      Vector Type: pPIC

      Contents & storage

      Store at -20°C.

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