pAO815 Pichia Expression Vector

Catalog number:  V18020

 Related applications: Protein Expression

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pAO815 is a Pichia expression vector designed to clone multiple copies of a gene into a single vector. The vector contains a Bgl II site upstream of the 5´ AOX1 gene and a unique BamH I site downstream of the 3´ AOX1 transcription termination (TT) signal. Four steps are required to generate multiple copies of the gene of interest:
1. The gene is cloned into the unique EcoR I site in the vector.
2. The construct is digested with BamH I and Bgl II to release the "expression cassette" containing the AOX1 promoter, gene of interest, and 3´ AOX1 TT.
3. Concatemers of the expression cassette are generated by ligation in vitro.
4. The multiple copies are inserted back into the pAO815 vector and transformed into Pichia. This vector is included in the Multi-Copy Pichia Expression Kit.
For Research Use Only. Not for use in diagnostic procedures.


Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cloning Method: Restriction Enzyme ⁄ MCS
Product Size: 20 µg
Promoter: AOX1
Protein Tag or Fusion: Untagged
Vector Type: pAO

Contents & storage

Store at -20°C.


Manuals & protocols