pPICZ A, B, & C Pichia Vectors

Catalog number: V19020

Invitrogen™  Related applications: Protein Expression

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Description

The pPICZ A, B, & C Pichia Vectors are designed for simple cloning and selection, high-level expression, and rapid detection and purification of the recombinant protein. These vectors contain the Zeocin™ resistance gene for direct selection of multi-copy integrant strains. By selecting with increasing amounts of Zeocin™, strains with multiple copies of your gene of interest integrated into the genome are obtained. Increasing the number of copies of the gene of interest in a recombinant Pichia strain can result in higher expression levels. The vectors are included in the EasySelect™ Pichia Expression Kit (Cat. No. K1740-01).

Features of the pPICZ vectors include:

• Inducible AOX1 promoter for high-level expression in Pichia pastoris
c-
myc epitope tag for convenient detection with an Anti-myc Antibody
• C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-His(C-term) Antibody
• Zeocin™ resistance for direct selection of multi-copy integrants

The pPICZ vector also features the É -factor secretion signal for efficient transport of proteins to the medium.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: AOX1
Vector Type: pPIC
Product Size: 20 µg
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: His Tag (6x), c-Myc Epitope Tag
Antibiotic Resistance (Bacterial): Zeocin™ (ZeoR)

Contents & storage

20 µg each of pPICZ A, B, & C suspended in 10 mM Tris–HCl, 1 mM EDTA, pH 8.0. A GS115/pPICZ/lacZ Positive Control strain is also included.

Store vectors at -5 to -30°C.

Documents

Manuals & protocols