Invitrogen™

      pPIC6 A, B, & C Pichia Vectors

      Catalog number:  V21020

       Related applications: Protein Expression

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      Description

      pPIC6 A, B, & C are Pichia pastoris expression vectors designed for simple selection,
      high-level expression, and rapid protein purification and detection. The pPIC6 A,
      B, & C vectors are designed for high-level secreted expression. Both sets of vectors
      have the following features:
      Blasticidin resistance for direct selection of multi-copy integrants
      Inducible AOX1 promoter for high-level expression in Pichia pastoris
       C-terminal c-myc epitope for convenient detection with an Anti-myc Antibody
      C-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating
      resin and detection with an Anti-His(C-term) Antibody
      pPIC6 also features:
      An É -factor secretion signal for efficient transport of proteins to the medium
      For Research Use Only. Not for use in diagnostic procedures.

      Figures

      Specifications

      Antibiotic Resistance (Bacterial): Blasticidin (BsdR)
      Cloning Method: Restriction Enzyme ⁄ MCS
      Product Size: 20 µg
      Promoter: AOX1
      Protein Tag or Fusion: His Tag (6x), c-Myc Epitope Tag
      Vector Type: pPIC

      Contents & storage

      Each vector kit includes 20 µg each of expression vector in A, B, & C versions, 20 µg of a lacZ (intracellular) or HSA (secreted) control vector, and an X-33 Pichia stab. Store vectors at -20°C. Store the Pichia stab at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

      Documents

      Manuals & protocols