pFLD1 Pichi pastoris Expression Vector Kit

Catalog number: V23020

Invitrogen™  Related applications: Protein Expression

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Description

pFLD and pFLD-alpha are vectors used to express recombinant proteins in Pichia pastoris. Recombinant proteins are expressed as fusions to a C-terminal peptide containing the V5 epitope and a polyhistidine (6xHis) tag.

The vector allows high-level, methanol- and methylamine-inducible expression of the gene of interest in Pichia, and can be used in any Pichia strain, including X-33, GS115, SMD1168H, and KM71H. The pFLD vectors contain the following elements:

• 5' fragment containing the FLD1 promoter for methanol- or methylamineinduced expression of the gene of interest
• Zeocin™ resistance gene for selection in both E. coli and Pichia
• Ampicillin resistance gene for selection in E. coli
• C-terminal peptide containing the V5 epitope and a polyhistidine (6xHis) tag for detection and purification of a recombinant fusion protein (if desired)

For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: FLD1
Vector Type: pFLD
Product Size: 1 kit
Cloning Method: Restriction Enzyme ⁄ MCS
Protein Tag or Fusion: His Tag (6x), V5 Epitope Tag
Antibiotic Resistance (Bacterial): Ampicillin (AmpR), Zeocin™ (ZeoR)

Contents & storage

Contains the following vectors, supplied at a concentration of 0.5 µg/µL in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 in a total volume of 40 µL:
• pFLD
• pFLD-alpha
• pFLD/CAT (control plasmid)

Shipped at room temperature. Upon receipt, store at -20°C.

Documents

Manuals & protocols