Vybrant® Apoptosis Assay Kit #6, Biotin-X Annexin V/Alexa Fluor® 350 Streptavidin/Propidium Iodide

Catalog number: V23200

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Chromatin Biology | Flow Cytometry | Target-Based ADME⁄Tox Assays

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Description

The Vybrant Apoptosis Assay Kit #6 uses the bright, blue-fluorescent Alexa Fluor 350 conjugate of streptavidin in conjunction with biotin-X annexin V to detect apoptotic cell populations either by flow cytometry or imaging. Alexa Fluor 350 streptavidin binds to biotin-X annexin V, which in turn binds to the phosphatidylserine on the surfaces of apoptotic cells. Necrotic cells are distinguished using red-fluorescent propidium iodide, which binds tightly to the nucleic acids in those cells. Propidium iodide does not penetrate the plasma membranes of live cells or early apoptotic cells. The kit contains an ample amount of each reagent for about 50 flow cytometric assays.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Solution
Format: Tube(s)
Label or Dye: Alexa Fluor™ 350, Propidium iodide, Biotin-X
Product Size: 50 assays
Detection Method: Fluorescent
Number of Reactions: 50
For Use With (Equipment): Flow Cytometer
Excitation⁄Emission (nm): PI: 535⁄617, Alexa Fluor® 350: 346⁄442
Shipping Condition: Wet Ice

Contents & storage

Contains 1 vial of biotin-x anneixn V, 1 vial of Alexa Fluor® 350 streptavidin, 1 vial of propidium iodide, and 1 bottle of 5x annexin binding buffer.

Store in refrigerator (2–8°C) and protect from light.

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