Chromatin Condensation & Membrane Permeability Dead Cell Apoptosis Kit with Hoechst 33342, YO-PRO®-1, and PI dyes, for flow cytometry

Catalog number: V23201

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Chromatin Biology | Flow Cytometry | Target-Based ADME⁄Tox Assays

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Description

This product detects apoptotic cells with changes in nuclear chromatin condensation and plasma membrane permeability, using three nucleic acid stains: UV-excitable Hoechst 33342, green fluorescent YO-PRO™ dye, and propidium iodide. The YO-PRO™ dye can enter apoptotic cells, whereas red-fluorescent propidium iodide (PI) cannot. Thus after staining with YO-PRO™-1 dye and PI, apoptotic cells show green fluorescence, and dead cells show primarily red fluorescence and some green fluorescence. Blue fluorescent Hoechst 33342 brightly stains the condensed chromatin of apoptotic cells and more dimly stains the normal chromatin of live cells. The staining pattern resulting from the simultaneous use of these three dyes makes it possible to distinguish normal, apoptotic and dead cell populations by flow cytometry or fluorescence microscopy.

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Form: Solution
Format: Tube(s)
Label or Dye: Propidium iodide, Hoechst 33342, YO-PRO®-1
Product Size: 1 kit
Detection Method: Fluorescent
Number of Reactions: 200
For Use With (Equipment): Flow Cytometer
Excitation⁄Emission (nm): YO-PRO®-1: 491⁄509, PI: 535⁄617, Hoechst 33342: 350⁄461
Shipping Condition: Room Temperature

Contents & storage

Contains 1 vial of Hoechst 33342, 1 vial of YO-PRO®-1, and propidium iodide.

Store in refrigerator (2–8°C) and protect from light.

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