Dead Cell Apoptosis Kit with Annexin V APC and SYTOX® Green, for flow cytometry

Catalog number: V35113

Molecular Probes™  Related applications: Cell Viability, Proliferation & Function | Cellular Imaging | Flow Cytometry | Target-Based ADME⁄Tox Assays

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Description

This flow cytometry product detects the externalization of phosphatidylserine in apoptotic cells using recombinant annexin V conjugated to red laser-excited allophycocyanin, and dead cells using SYTOX™ Green nucleic acid stain. Apoptotic cells are detected by annexin V binding to externalized phosphatidylserine, and late apoptotic and necrotic cells have compromised membranes that permit SYTOX™ Green stain access to cellular nucleic acids. After exposing a cell population to APC annexin V and SYTOX™ Green stain, live cells show little or no fluorescence, apoptotic cells show red fluorescence and very little green fluorescence, and late apoptotic cells show a higher level of red and orange fluorescence. These populations can easily be distinguished using a flow cytometer that has both 488 nm and 633 nm excitation sources (an argon-ion laser and a HeNe laser).

View a selection guide for all apoptosis assays for flow cytometry.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Format: Slide(s), Tube(s)
Label or Dye: APC (Allophycocyanin), SYTOX® Green
Product Size: 1 kit
Detection Method: Fluorescent
Number of Reactions: 50
For Use With (Equipment): Fluorescence Microscope, Flow Cytometer
Excitation⁄Emission (nm): APC: 650⁄660, SYTOX® green: 503⁄524
Shipping Condition: Wet Ice

Contents & storage

Contains 1 vial of annexin V, APC conjugate (250 µL), 1 vial of SYTOX® green stain (100 µL),and 1 bottle of annexin binding buffer (5X solution, 15 mL) .

Store in refrigerator (2–8°C) and protect from light.

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