Vivid Colors™ pcDNA™6.2/C-YFP-DEST Vector

Catalog number:  V35720

 Related applications: Cellular Imaging | Gateway Cloning | Protein Assays and Analysis | Protein Expression

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The Vivid Colors™ Fluorescent Protein Gateway® Destination Vectors allow you to quickly and easily fuse a protein of interest to the widely used and well-characterized fluorescent proteins from the jellyfish Aequorea victoria (1,2) using the pcDNA™ 6.2 Gateway™ mammalian expression vector. These powerful Gateway® Technology vectors contain the next-generation EGFP, Emerald Green Fluorescent Protein (EmGFP), or the popular Yellow Fluorescent Protein (YFP) for simple, non-invasive detection of recombinant protein. Both fluorescent proteins have been humanized for optimal mammalian expression (3). In addition, the Vivid Colors™ pcDNA™ 6.2 Fluorescent Protein Gateway® Vectors offer:

• CMV promoter for high-level expression of the recombinant fluorescent fusion protein
• Options to fuse EmGFP or YFP to the N- and C-terminus of your protein
For Research Use Only. Not for use in diagnostic procedures.


Promoter: CMV
Key Function: Reporter Assays, Subcellular Localization
Protein Tag or Fusion: YFP
Reporter: YFP
Delivery Method: Transfection
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pDEST, pcDNA
Constitutive or Inducible System: Constitutive
Product Line: Gateway®, Vivid Colors™, pcDNA™
Product Size: 6 µg

Contents & storage

Each vector is supplied as supercoiled and lyophilized plasmid DNA. Store at -20°C after resuspended. Guaranteed stable for 6 months when properly stored.


Manuals & protocols

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