pBAD/His Kit

Catalog number: V43001

Invitrogen™  Related applications: Protein Expression

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Description

The pBAD/His Kit provides all of the necessary reagents to express your protein in a tightly regulated fashion. The vector pBAD/His allows you to express your protein with an N-terminal tag. The vector provides:

• The araBAD promoter for tightly regulated expression
• Translation initiation signals optimized for E. coliexpression
• N-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin and detection with an Anti-HisG Antibody
• N-terminal Xpress™ epitope for detection and analysis with an Anti-Xpress™ Antibody
• Enterokinase cleavage site for removing the N-terminal tag following purification

Three vectors are provided (A, B, and C). Each has the N-terminal tag in a different reading
frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Vector Type: pBAD
Cloning Method: Restriction Enzyme ⁄ MCS
Promoter: araBAD
Protein Tag or Fusion: His Tag (6x)
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Cleavage: EK (Enterokinase) Recognition Site
Product Size: 1 kit
Inducing Agent: Arabinose
Selection Agent (Eukaryotic): None
Constitutive or Inducible System: Inducible

Contents & storage

The pBAD/His Kit includes 20 µg of each pBAD/His A, B, & C vector, 20 µg of pBAD/His/lacZ, 1 ml of 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Vectors and 20% L-arabinose should be stored at -20°C. Store LMG194 and TOP10 stabs at 2 - 8°C. All components are guaranteed stable for 6 months when properly stored.

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