pBAD/gIII Kit

Catalog number: V45001

Invitrogen™  Related applications: Protein Expression

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Description

The pBAD/gIII Kit provides all of the necessary reagents to express recombinant proteins under the tight regulation of the araBAD promoter. To simplify purification, the vector pBAD/gIII encodes a leader peptide which directs the recombinant protein into the
periplasmic space. The vector provides:

• The araBAD promoter for tightly regulated expression
• Translation initiation signals for optimized E. coliexpression
• Leader peptide from the bacteriophage fd gene III protein (gIII) for secreted expression
• C-terminal polyhistidine (6xHis) tag for purification with nickel-chelating resin
• C-terminal c-myc epitope for detection and analysis with an Anti-myc Antibody

A set of three vectors is provided (A, B, and C). Each has the C-terminal tag in a different reading frame relative to the multiple cloning site to simplify in-frame cloning of your gene.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Vector Type: pBAD
Cloning Method: Restriction Enzyme ⁄ MCS
Promoter: araBAD
Protein Tag or Fusion: c-Myc Epitope Tag
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)
Product Size: 1 kit
Inducing Agent: Arabinose
Selection Agent (Eukaryotic): None
Constitutive or Inducible System: Inducible

Contents & storage

The pBAD/gIII Kit includes 20 µg each of pBAD/gIII A, B, & C, 20 µg of pBAD/gIII/calmodulin, 1 ml 20% L-arabinose, LMG194 and TOP10 E. coli stabs. Store stabs at 2 - 8°C. Store all other components at -20°C. All components are guaranteed stable for 6 months when properly stored.

Documents

Manuals & protocols