BLOCK-iT™ Lentiviral RNAi Zeo Gateway™ Vector Kit

      Numéro de catalogue:  V48820

      Invitrogen™  Applications liées: RNAi

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      Description

      The BLOCK-iT™ Lentiviral RNAi Zeo Gateway® Vector Kit contains the pLenti4/BLOCK-iT™-DEST expression vector which enables lentiviral delivery and genomic integration of DNA coding for shRNA. Once expressed, the shRNA is processed by cellular machinery and initiates target-specific RNAi. The pLenti4/BLOCK-iT™-DEST vector offers:

      Gateway® Technology for efficient recombination of the RNAi cassette from the BLOCK-iT™ inducible pENTR™/H1/TO vector

      • All required components for efficient lentiviral packaging, delivery, and integration of the shRNA
      • Zeocin™ selection marker for fast selection of clonal cell lines containing the RNAi cassette
      For Research Use Only. Not for use in diagnostic procedures.

      Spécifications

      Cloning Method: Gateway®
      Constitutive or Inducible System: Inducible
      Delivery Method: Lentiviral
      Product Line: BLOCK-iT™, Gateway®
      Product Size: 20 reactions
      Promoter: H1⁄TO
      RNAi Type: shRNA
      Selection Agent (Eukaryotic): Zeocin™
      Vector Type: pLenti

      Contenu et stockage

      The BLOCK-iT™ Lentiviral RNAi Zeo Gateway® Vector Kit contains the pLenti4/BLOCK-iT™-DEST vector, pLenti4-GW/H1/TO-lamin shRNA control vector, and One Shot® Stbl3™ Chemically Competent E.Coli. Store the pLenti4/BLOCK-iT™-DEST, pLenti6/TR vector, Blasticidin, ViraPower™ Lentiviral packaging mix, and the control vector at -20°C. Store the One Shot Stbl3™ Competent Cells 293 FT cell, and LR Clonase® enzyme mix at -80°C. Store Lipofectamine™ 2000 at +4°C. All reagents are guaranteed stable for 6 months when properly stored.

      Documents

      Manuels et protocoles

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