pFRT/lacZeo Vector

Catalog number:  V601520

 Related applications: Protein Expression

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pFRT/lacZeo and pFRT/lacZeo2 were constructed for establishing Flp-In™ Cell Lines that contain a single FRT site. Each vector includes a:

• FRT site for subsequent targeted integration of a Flp-In™ expression vector
Z-Zeocin™ fusion for selection of stable integrants with Zeocin™

These vectors differ in the SV40 promoter that drives expression of the lacZ-Zeocin™ fusion. pFRT/lacZeo2 contains a truncated version of the SV40 promoter. Use of this vector facilitates the isolation of clones that have integrated the vector near enhancer elements in the genome. Selection of stable clones using pFRT/lacZeo2 may allow easier isolation of clones that express a gene of interest at higher levels.
For Research Use Only. Not for use in diagnostic procedures.


Promoter: SV40
Key Function: Targeted Integration
Protein Tag or Fusion: Untagged
Reporter: Beta-Gal (lacZ)
Delivery Method: Transfection
Selection Agent (Eukaryotic): Zeocin™
Vector Type: pFRT
Constitutive or Inducible System: Constitutive
Product Line: Flp-In™
Product Size: 20 µg

Contents & storage

20 µg of vector, either pFRT/lacZeo or pFRT/lacZeo2, is provided supercoiled and lyophilized. Store at -20°C once resuspended. Vectors are guaranteed stable for 6 months when properly stored.


Manuals & protocols