pEF5/FRT/V5-DEST™ Gateway™ Vector

Catalog number: V602020

Invitrogen™  Related applications: Gateway Cloning | Protein Expression

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Description

To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway® destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway® destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ RF Clone. The following table lists the wide range of destination vectors available.

Additional materials required, available separately: Gateway® entry clone, Gateway® LR Clonase® enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: EF-1α
Key Function: Targeted Integration
Protein Tag or Fusion: V5 Epitope Tag
Delivery Method: Transfection
Cloning Method: Gateway®
Selection Agent (Eukaryotic): Hygromycin
Vector Type: pDEST
Product Size: 6 µg
Constitutive or Inducible System: Constitutive

Contents & storage

All destination vectors are provided lyophilized and supercoiled.

Documents

Manuals & protocols

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