pFRT/lacZeo2 Vector

Catalog number: V602220

Invitrogen™  Related applications: Protein Expression

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Description

pFRT/lacZeo and pFRT/lacZeo2 were constructed for establishing Flp-In™ Cell Lines that contain a single FRT site. Each vector includes a:

• FRT site for subsequent targeted integration of a Flp-In™ expression vector
Z-Zeocin™ fusion for selection of stable integrants with Zeocin™

These vectors differ in the SV40 promoter that drives expression of the lacZ-Zeocin™ fusion. pFRT/lacZeo2 contains a truncated version of the SV40 promoter. Use of this vector facilitates the isolation of clones that have integrated the vector near enhancer elements in the genome. Selection of stable clones using pFRT/lacZeo2 may allow easier isolation of clones that express a gene of interest at higher levels.
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: SV40
Key Function: Targeted Integration
Protein Tag or Fusion: Untagged
Delivery Method: Transfection
Selection Agent (Eukaryotic): Zeocin™
Vector Type: pFRT
Product Size: 20 µg
Constitutive or Inducible System: Constitutive

Contents & storage

20 µg pFRT/lacZeo2 is supplied at 0.5 µg/µL in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0 in a total volume of 40 µL.

Documents

Manuals & protocols