pTracer™-CMV/Bsd Mammalian Expression Vector

Catalog number: V88320

Invitrogen™  Related applications: Cellular Imaging | Protein Assays and Analysis | Protein Expression

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Description

Two Tracer™ mammalian expression vectors are available that express GFP fused to the selectable marker Blasticidin. Blasticidin is a potent selection agent that allows a more rapid generation of stable cell lines than other selection agents. Each vector uses a different set of promoters to express the gene of interest and the Cycle 3 GFP-Blasticidin resistance gene fusion.

The pTracer™-CMV/Bsd vector offers:

• CMV promoter for high-level constitutive expression of your gene of interest
• EF-1 promoter for high-level constitutive expression of the Cycle 3 GFP-Blasticidin resistance fusion

The pTracer™-EF/Bsd vectors offer:
• EF-1 promoter for high-level constitutive expression of your gene of interest
• CMV promoter for high-level constitutive expression of the Cycle 3 GFP-Blasticidin resistance fusion
• C-terminal V5 epitope tag for simple detection of recombinant protein with an Anti-V5 Antibody
• C-terminal 6xHis tag for rapid purification on nickel-chelating resin and simple detection with an Anti-His(C-term) Antibody
• Three reading frames for easier in-frame cloning
For Research Use Only. Not for use in diagnostic procedures.

Figures

Specifications

Promoter: CMV
Key Function: Reporter Assays
Protein Tag or Fusion: Untagged
Reporter: GFP (Cycle 3)
Delivery Method: Transfection
Cloning Method: Restriction Enzyme ⁄ MCS
Selection Agent (Eukaryotic): Blasticidin
Vector Type: pTracer
Product Size: 20 µg
Constitutive or Inducible System: Constitutive

Contents & storage

20 µg each of the pTracer™ /Bsd vector and a lacZ expression control are provided supercoiled and lyophilized. pTracer™ /Bsd Kits contain the appropriate pTracer™ vector and 50 mg of the selection agent Blasticidin. Store vectors and Blasticidin at -20°C. All reagents are stable for 6 months when properly stored.

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