pEXP-5-CT/TOPO® TA Expression Kit

Catalog number: V96006

Invitrogen™  Related applications: Protein Expression

Error loading your content!

  Catalog number
Select a plan
Unit size
Price ({{currency}}) Your price ({{currency}}) Availability Qty
{{product.sku}} {{product.sku}}
also known as {{product.formattedSku}} 
{{subscriptionDetails.selectedRatePlan.billingPeriod}} {{subscriptionDetails.selectedRatePlan.billingPeriod}} {{product.availability.message}}
Pro add-ons

Your on-site stock

›› {{supplyCenter.scName}}({{scProduct.stockOnHand}} In stock)
›› {{supplyCenter.scName}}(Out of stock)
›› {{supplyCenter.scName}}
This item is not currently available on-site. Depending on your Supply Center settings you may be able to add the item to cart above else use the Order Non-Stocked Items' tab on the Supply Center home page.
Back to top

Description

The pEXP5-CT/TOPO® TA Expression Kit provides a highly efficient, five-minute, one-step cloning strategy for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for T7-based, high-level expression of recombinant fusion proteins in the Expressway™ Maxi Cell-Free E. coli Expression System or for inducible expression in E. coli. No ligase, post-PCR procedures, or PCR primers containing special sequences are required to generate the expression construct.

Features of the pEXP5-CT/TOPO® vector include:

• Bacteriophage T7 promoter for high-level, inducible expression of the recombinant protein of interest
• C-terminal fusion tag for detection and purification of recombinant fusion proteins
• TOPO® Cloning site for rapid and efficient cloning of Taq-amplified PCR products
• Ampicillin resistance gene for selection in E. coli
• pUC origin for high-copy replication and maintenance of the plasmid in E. coli
For Research Use Only. Not for use in diagnostic procedures.

Specifications

Promoter: T7
Expression: E. coli
Vector Type: pEXP, TOPO-TA Vectors
Product Size: 10 reactions
Cloning Method: TOPO®-TA
Expression Mechanism: Cell-Based Expression
Protein Tag or Fusion: His Tag (6x)
Bacterial or Yeast Strain: TOP10
Selection Agent (Eukaryotic): None
Antibiotic Resistance (Bacterial): Ampicillin (AmpR)

Contents & storage

The pEXP-5-CT/TOPO® TA Expression Kit includes vector, 10X PCR Buffer, dNTP Mix, Salt Solution, Water, T7 Forward Primer, T7 Term Reverse Primer, Control PCR Primers, and Control PCR Template.

Documents

Manuals & protocols