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LSIVet™ BVD/BD Antigen Skin LeucoC Organs ELISA Kit

Catalog number: VETBVDAG1

 Related applications: Animal Health Immunoassays

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Description

Single-well Antigen-capture ELISA kits enable sensitive and specific detection of pathogens in animal samples, allowing for reliable and rapid screening and detection of infected animals.

How the kit works:

1. Samples are added to the microplate coated with anti-pathogen antibodies. If present in the sample, specific antigen binds to the anti-pathogen antibodies and form antigen-antibody complexes.
2. After washing, an anti-pathogen peroxidase-labeled conjugate is added, which binds to any antigens attached to the plate.
3. After a second washing, a chromogenic substrate is added. Color development results from oxidation of the substrate by the conjugated peroxidase.
4. After stopping the reaction, results are read using an ELISA plate reader. Positive samples will show color. The amount of color in each well is proportional to the amount of specific antigen in the sample.

For veterinary use only.

Regulatory requirements vary by country; products may not be available in your geographic area.

Biological hazard safety

WARNING! BIOHAZARD. Biological samples such as tissues, body fluids, infectious agents, and blood of humans and other animals have the potential to transmit infectious diseases. All work should be conducted in properly equipped facilities using the appropriate safety equipment (for example, physical containment devices). Safety equipment also may include items for personal protection, such as gloves, coats, gowns, shoe covers, boots, respirators, face shields, safety glasses, or goggles. Individuals should be trained according to applicable regulatory and company/institution requirements before working with potentially biohazardous materials. Follow all applicable local, state/provincial, and/or national regulations. The following references provide general guidelines when handling biological samples in laboratory environment.

U.S. Department of Health and Human Services, Biosafety in Microbiological and Biomedical Laboratories (BMBL), 5th Edition, HHS Publication No. (CDC) 21-1112, Revised December 2009

World Health Organization, Laboratory Biosafety Manual, 3rd Edition, WHO/CDS/CSR/LYO/2004.11
For Veterinary Use Only.

Specifications

Target Organism Class: Bovine Viral Diarrhea Virus (BVDV), Border Disease Virus (BDV)
Validated Application: ELISA
Detection Method: Colorimetric
Sample Type (Specific): Skin, Any Organ Type, Leukocytes
Species: Cattle⁄Bovine, Sheep⁄Ovine, Goat⁄Caprine, All Sensitive Animals
Product Size: 1 x 96 well plate

Documents

Manuals & protocols