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A novel, highly sensitive fluorescent dye–based system for N-glycan analysis

Glycosylation is one of the key critical quality attributes of monoclonal antibody–based biotherapeutics. Glycosylation changes can impact a biological drug’s safety, efficacy, clearance, and immunogenicity, making it necessary to accurately detect changes. Glycan profiling begins at cell line development, continues through process development, and, in certain cases, continues through drug substance release. Current glycan analysis methods involve laborious multistep sample preparation that takes anywhere from a day to multiple days for 96 samples, followed by single-channel LC or CE separation.

Glycan-Product-Shot

The Applied Biosystems™ GlycanAssure™ Glycan Analysis and Quantitation System is the first glycan analysis platform that combines both high throughput and high data quality, and offers an integrated glycan analysis platform that helps save labor, time, and cost of analysis. The GlycanAssure system offers simple and easy magnetic bead–based sample preparation with multiple fluorescent dyes for glycan labeling; multi-capillary CE instruments for high-throughput CE-LIF–based glycan analysis; and assay-specific software for fast data analysis and reporting.

Features and benefits:

  • Performance—resolution of sialylated glycans, structural isomers, fucose species, and high-mannose species
  • Ease of use—magnetic bead–based sample preparation using less labor (reduced pipetting and hands-on time); no use of sodium cyanoborohydride; and no vacuum centrifugation steps
  • Data quality—multiple fluorescent dyes for superior sensitivity and selectivity—traditional APTS and the novel Teal™ and Turquoise™ dyes for glycan labeling
  • Throughput—sample preparation and analysis of 96 samples in 7–9 hours
  • Support—full field applications support at every stage of method implementation; system based on proven Applied Biosystems™ CE technology platform from an established, consistent supplier

Webinars

High Throughput and High Resolution N-glycan Analysis Using Multicapillary CE and Novel Fluorescent Dyes.

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Discover a Simple, Robust, and Automated 3-step Workflow N-glycan Analysis on UHPLC and Multicapillary CE

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GlycanAssure Software is easy-to-use data acquisition and analysis software for glycans with security, auditing, and E-signature (SAE) features. It breaks new ground in user-friendly navigation with an intuitive dashboard design, highly visible buttons for common operations, easy-to-read graphical displays to monitor the state of consumables, simplified plate setup, and a handy maintenance scheduling calendar functionality.

GlycanAssure Data Analysis software allows quick viewing of analyzed and un-analyzed glycan samples and quick comparison of different analysis methods. It includes built-in primary analysis with quality control, so you can make decisions about the quality of data as it is produced without the need to transfer output files to other software.

GlycanAssure Software has a full suite of security, auditing, and E-signature features to help enable 21 CFR part 11 compliance.

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Data acquisition software

Data acquisition software

Glycan Analysis Software

Glycan Analysis Software

GlycanAssure sample prep kits provide three fluorescent dye options for glycan labeling, including the traditional APTS dye and two new dyes with novel features to resolve difficult-to-separate glycans. The highly sensitive Teal and Turquoise dyes enable analysis of low-input glycoproteins and quantitation of glycans present at low levels. The GlycanAssure Teal labeling protocol doesn’t require removal of excess dye and provides data for 96 samples in 7 hours.

GlycanAssure APTS Kit

GlycanAssure Teal Kit

GlycanAssure Turquoise Kit

The 3500 Genetic Analyzer for Protein Quality Analysis is a capillary electrophoresis instrument that enables parallel analysis of 8 or 24 samples without compromising on the run time per sample, thereby enabling high throughput analysis with high quality data.

  • 8 or 24-capillary system for high-throughput analysis
  • Simple setup, operation, and maintenance
  • Radio frequency identification (RFID) technology that tracks key consumables data and records administrative information
  • Signal uniformity from instrument-to-instrument, run-to-run, and capillary array-to-capillary arra

GlycanAssure Software is easy-to-use data acquisition and analysis software for glycans with security, auditing, and E-signature (SAE) features. It breaks new ground in user-friendly navigation with an intuitive dashboard design, highly visible buttons for common operations, easy-to-read graphical displays to monitor the state of consumables, simplified plate setup, and a handy maintenance scheduling calendar functionality.

GlycanAssure Data Analysis software allows quick viewing of analyzed and un-analyzed glycan samples and quick comparison of different analysis methods. It includes built-in primary analysis with quality control, so you can make decisions about the quality of data as it is produced without the need to transfer output files to other software.

GlycanAssure Software has a full suite of security, auditing, and E-signature features to help enable 21 CFR part 11 compliance.

Request software ›

Data acquisition software

Data acquisition software

Glycan Analysis Software

Glycan Analysis Software

GlycanAssure sample prep kits provide three fluorescent dye options for glycan labeling, including the traditional APTS dye and two new dyes with novel features to resolve difficult-to-separate glycans. The highly sensitive Teal and Turquoise dyes enable analysis of low-input glycoproteins and quantitation of glycans present at low levels. The GlycanAssure Teal labeling protocol doesn’t require removal of excess dye and provides data for 96 samples in 7 hours.

GlycanAssure APTS Kit

GlycanAssure Teal Kit

GlycanAssure Turquoise Kit

The 3500 Genetic Analyzer for Protein Quality Analysis is a capillary electrophoresis instrument that enables parallel analysis of 8 or 24 samples without compromising on the run time per sample, thereby enabling high throughput analysis with high quality data.

  • 8 or 24-capillary system for high-throughput analysis
  • Simple setup, operation, and maintenance
  • Radio frequency identification (RFID) technology that tracks key consumables data and records administrative information
  • Signal uniformity from instrument-to-instrument, run-to-run, and capillary array-to-capillary arra

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