Powerful molecular testing across the drug development continuum—from preclinical to companion diagnostics

Genomics plays an essential role in oncology drug discovery and companion diagnostics development. The Oncomine® Cancer Research Panel and Workflow is a fast, easy-to-use, next-generation sequencing tool enabling the analysis of hundreds of genes in a single run, providing a universal solution for use in your drug and companion diagnostics development efforts.

Oncomine® Cancer Research Panel and Workflow—one targeted next-generation sequencing tool for multiple drug and companion Dx development programs

The Oncomine® Cancer Research Panel and Workflow is based on the proven Ion Torrent™ next-generation sequencing platform and powerful Ion AmpliSeq™ library preparation technology, and also leverages the Oncomine® Knowledgebase, a rich cancer genomics data resource, for target selection, variant calling, and data annotations. Features include:

  • Minimal sample input required per run—making it well suited for analysis of precious tumor samples
  • Generates data for hundreds of important biomarkers in a single run, targeting well-referenced hotspot mutations, CNVs, gene fusions, and indels
  • Easy-to-perform workflow with fast turn-around time—get your NGS data easily and quickly

Learn more below or request to be contacted by a member of our clinical development team.

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The workflow begins with nucleic acid extraction from FFPE samples, followed by library preparation using the Oncomine® Cancer Research Panel based on proven Ion AmpliSeq™ technology. The highly informative biomarker targets in the panel were carefully selected by leveraging the expertly curated cancer genomics data within the comprehensive Oncomine® Knowledgebase. Next-generation sequencing is then completed quickly and easily using the Ion PGM™ System. The resulting variant data—including hotspot mutations, CNVs, gene fusions, and indels—are annotated using the Oncomine® Cancer Research Panel Knowledgebase data analysis tool, enabling users to prepare a report meeting their specific laboratory needs.

Discuss the Oncomine® Cancer Research Panel and Workflow with a member of our clinical development team.

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Ion AmpliSeq™ technology delivers simple and fast library construction for targeted sequencing of specific human genes or genomic regions. Based on ultrahigh-multiplex PCR, Ion AmpliSeq™ technology requires as little as 10 ng of input DNA per reaction to target sets of genes, including SNPs, CNVs, gene fusions, and indels, making sequencing of FFPE samples routine on the Ion PGM™ System.

Alternative target selection methods are lengthy and complex and require large amounts (100s of nanograms) of DNA, making them impractical to routinely sequence DNA or RNA from the formalin-fixed, paraffin-embedded (FFPE) tissues commonly used in clinical cancer research, drug, and companion diagnostic development.

Because Ion AmpliSeq™ technology can amplify hundreds of biomarker targets in a single reaction, the Oncomine® Cancer Research Panel can comprehensively analyze hundreds of the most referenced oncology biomarkers using just 3 primer pools. This not only enables a fast and easy workflow, but also helps ensure that important, actionable—but less prevalent— cancer variants are not missed.

With the power of Ion AmpliSeq™ technology, the Oncomine® Cancer Research Panel can become your universal test for oncology clinical trials and companion diagnostic development.

Discuss the Oncomine® Cancer Research Panel with member of our clinical development team.

Ion Torrent™ NGS platform is the solution to advance oncology drug and companion diagnostic development

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Learn more about Ion Torrent™ next-generation sequencing technology.

The Ion PGM™ System combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and making it accessible to virtually any laboratory. The system’s use of the simplest, natural sequencing chemistry eliminates the need for complicated optics and detection systems, and reduces complex chemistries to measure natural DNA extension. Direct, real time sequencing detection provides sequencing results typically in less than 3 hours.

Discuss the Oncomine® Cancer Research Panel and Ion PGM™ System with a member of our clinical development team.

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Variant and Actionability Lookup Databses

Genetic and Variant Reported Summaries

Oncomine® Cancer Research Panel Knowledgebase

To inform and drive drug discovery and companion diagnostic development programs, the vast genomic data sets produced by next-generation sequencing studies must be analyzed, annotated, and, ultimately, interpreted by experienced bioinformaticians. The Oncomine® Knowledgebase, the world’s largest curated compendium of cancer genomic information, is a database of integrated cancer genomics data with exceptional attention to detail. The Oncomine® Knowledgebase was an instrumental tool in the selection of targets and the development of the Oncomine® Cancer Research Panel.

Powered by the Oncomine® Knowledgebase, the Oncomine® Cancer Research Panel Knowledgebase was developed specifically for further examination of next-generation sequencing data generated from samples analyzed using the Oncomine® Cancer Research Panel and Workflow. Users may subsequently create data summaries and reports appropriate for their studies.

The Oncomine® Cancer Research Panel Knowledgebase is a curated set of published clinical evidence that supports the matching of driver genetic variants with relevant clinical therapeutic options, such as published therapeutic interventions and clinical trials. The Oncomine® Cancer Research Panel Knowledgebase is comprised of two component tools:

  • Variant & Actionability Lookup Databases
    • 4 data lookup tables in tab separated (.TSV) file format
    • Oncomine® driver, prevalence, and actionability content for individual variants
  • Genetic & Variant Reported Summaries
    • Pre-rendered supplementary information tables in PDF format
    • Summary files for variant actionability class and cancer type

Discuss the Oncomine® Cancer Research Panel Knowledgebase with member of the clinical development team.

Custom Image: Double click to configure component

The workflow begins with nucleic acid extraction from FFPE samples, followed by library preparation using the Oncomine® Cancer Research Panel based on proven Ion AmpliSeq™ technology. The highly informative biomarker targets in the panel were carefully selected by leveraging the expertly curated cancer genomics data within the comprehensive Oncomine® Knowledgebase. Next-generation sequencing is then completed quickly and easily using the Ion PGM™ System. The resulting variant data—including hotspot mutations, CNVs, gene fusions, and indels—are annotated using the Oncomine® Cancer Research Panel Knowledgebase data analysis tool, enabling users to prepare a report meeting their specific laboratory needs.

Discuss the Oncomine® Cancer Research Panel and Workflow with a member of our clinical development team.

Custom Image: Double click to configure component

Ion AmpliSeq™ technology delivers simple and fast library construction for targeted sequencing of specific human genes or genomic regions. Based on ultrahigh-multiplex PCR, Ion AmpliSeq™ technology requires as little as 10 ng of input DNA per reaction to target sets of genes, including SNPs, CNVs, gene fusions, and indels, making sequencing of FFPE samples routine on the Ion PGM™ System.

Alternative target selection methods are lengthy and complex and require large amounts (100s of nanograms) of DNA, making them impractical to routinely sequence DNA or RNA from the formalin-fixed, paraffin-embedded (FFPE) tissues commonly used in clinical cancer research, drug, and companion diagnostic development.

Because Ion AmpliSeq™ technology can amplify hundreds of biomarker targets in a single reaction, the Oncomine® Cancer Research Panel can comprehensively analyze hundreds of the most referenced oncology biomarkers using just 3 primer pools. This not only enables a fast and easy workflow, but also helps ensure that important, actionable—but less prevalent— cancer variants are not missed.

With the power of Ion AmpliSeq™ technology, the Oncomine® Cancer Research Panel can become your universal test for oncology clinical trials and companion diagnostic development.

Discuss the Oncomine® Cancer Research Panel with member of our clinical development team.

Ion Torrent™ NGS platform is the solution to advance oncology drug and companion diagnostic development

Custom Image: Double click to configure component

Learn more about Ion Torrent™ next-generation sequencing technology.

The Ion PGM™ System combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data, democratizing sequencing and making it accessible to virtually any laboratory. The system’s use of the simplest, natural sequencing chemistry eliminates the need for complicated optics and detection systems, and reduces complex chemistries to measure natural DNA extension. Direct, real time sequencing detection provides sequencing results typically in less than 3 hours.

Discuss the Oncomine® Cancer Research Panel and Ion PGM™ System with a member of our clinical development team.

Custom Image: Double click to configure component

Variant and Actionability Lookup Databses

Genetic and Variant Reported Summaries

Oncomine® Cancer Research Panel Knowledgebase

To inform and drive drug discovery and companion diagnostic development programs, the vast genomic data sets produced by next-generation sequencing studies must be analyzed, annotated, and, ultimately, interpreted by experienced bioinformaticians. The Oncomine® Knowledgebase, the world’s largest curated compendium of cancer genomic information, is a database of integrated cancer genomics data with exceptional attention to detail. The Oncomine® Knowledgebase was an instrumental tool in the selection of targets and the development of the Oncomine® Cancer Research Panel.

Powered by the Oncomine® Knowledgebase, the Oncomine® Cancer Research Panel Knowledgebase was developed specifically for further examination of next-generation sequencing data generated from samples analyzed using the Oncomine® Cancer Research Panel and Workflow. Users may subsequently create data summaries and reports appropriate for their studies.

The Oncomine® Cancer Research Panel Knowledgebase is a curated set of published clinical evidence that supports the matching of driver genetic variants with relevant clinical therapeutic options, such as published therapeutic interventions and clinical trials. The Oncomine® Cancer Research Panel Knowledgebase is comprised of two component tools:

  • Variant & Actionability Lookup Databases
    • 4 data lookup tables in tab separated (.TSV) file format
    • Oncomine® driver, prevalence, and actionability content for individual variants
  • Genetic & Variant Reported Summaries
    • Pre-rendered supplementary information tables in PDF format
    • Summary files for variant actionability class and cancer type

Discuss the Oncomine® Cancer Research Panel Knowledgebase with member of the clinical development team.

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