Making the undetectable, detectable

Making the undetectable, detectable

Improve separation and detectability of a compound destined for GC or GC-MS analysis using this selection of derivatization reagents. We also offer siliconizing fluids to coat various lab consumables with an inert, water-repellent film. All Thermo Scientific™ GC reagents offer the variety, quality and reliability you need.

Popular gas chromatography derivatization reagents
GC reagents

Silylation Reagents

The most widely used derivatives for GC applications
BSTFA and BSTFA + TMCS For excellent separations and difficult-to-silylate compounds.
MSTFA and MSTFA + 1% TMCS Offers maximum volatility. Ideal for preparing volatile and thermally stable derivatives for GC and GC-MS.
BSA The perfect reagent for rapid silylation reactions. Ideal for alcohols, amines, amides, carboxylic acids, phenols, steroids, biogenic amines and alkaloids.
MTBSTFA and MTBSTFA + 1% TBDMCS Silylation Reagent Offers stable TBDMS (tert-butyldimethylsilyl) derivatization of hydroxyl, carboxyl, thiol and primary and secondary amines with typical yields over 96%.
TMSI (N-Trimethylsilylimisazole) The strongest silylator for carbohydrates and steroids Reacts quickly and smoothly with hydroxyls and carboxylic acids, but not with amines.
HMDS (Hexamethyldisilazane) The popular choice for silylation of sugars and related substances to extend the practical range of GC and improve chromatographic results.
MOX (Methoxamine) Useful for preparing oximes of steroids and ketoacids prior to silylation
Tri-Sil HTP (HMDS:TMCS:Pyridine) Reagent-catalyst mixture for one-step derivatization of carbohydrates, phenols, steroids, sterols, organic acids, alcohols and some amines
Tri-Sil BP (BSA:Pyridine) Derivatizes alcohols, phenols, organic acids, aromatic amides and amines
Tri-Sil TBT (TMSI:BSA:TMCS) A catalyzed silylation reagent formulation containing three parts TMSI, three parts BSA and two parts TMCS
Tri-Sil TP (TMSI:Pyridine) Derivatizes hydroxyl compounds, particularly carbohydrates
TMCS (Trimethylchlorosilane) An excellent catalyst for difficult-to-silylate compounds. Excellent adjunct for forming trimethylsilyl ethers for GC determinations.
Silylation Grade Solvents Manufactured for the exacting needs of silylation and other sensitive derivatization reactions.

Acylation Reagents

Converts compounds that contain active hydrogens through the action of a carboxylic acid or a carboxylic acid derivative
Pentafluoropropanol Purified for GC-MS applications, The addition of fluorine greatly enhances the sensitivity of certain detectors.
MBTFA (N-Methyl-bis(trifluoroacetamide) For trifluoroacylating primary and secondary amines, hydroxyl and thiol groups and carbohydrates.
Perfluoroacylimidazoles (HFBI and TFAI) Offer effective acylation of hydroxyl groups and primary and secondary amines.
Perfluoro Acid Anhydrides (TFAA, PFAA and HFAA) Highly purified for optimal preparation of fluoracyl derivatives.

Alkylation Reagents

Substitution of an active hydrogen by an aliphatic or aliphatic-aromatic (benzyl) group for modifying compounds containing acidic hydrogens, such as carboxylic acids and phenols.
BF3-Methanol Provides convenient, fast and quantitative esterification of fatty acids.
Pentafluorobenzyl Bromide (PFBBr) For electron capture GC analysis of carboxyl acids, phenols and sulfonamides.
Methylate (DMFDMA) For easy, effective preparation of methyl esters from fatty acids and amino acids.
MethElute (TMPAH) Alylation Reagent Provides accurate sensitive on-column methylation.

Siliconizing Fluids

Coat various lab consumables with an inert, water-repellent film.
Water-Soluble Siliconizing Fluid Attaches the silane polymer, octadecyltrialkosilane, to make the surface inert or polymerizes to create an inert film.
Hydrocarbon-Soluble Siliconizing Fluid Attaches a short-chain silane polymer to make the surface inert or polymerizes to create an inert film.

Silylation Reagents

The most widely used derivatives for GC applications
BSTFA and BSTFA + TMCS For excellent separations and difficult-to-silylate compounds.
MSTFA and MSTFA + 1% TMCS Offers maximum volatility. Ideal for preparing volatile and thermally stable derivatives for GC and GC-MS.
BSA The perfect reagent for rapid silylation reactions. Ideal for alcohols, amines, amides, carboxylic acids, phenols, steroids, biogenic amines and alkaloids.
MTBSTFA and MTBSTFA + 1% TBDMCS Silylation Reagent Offers stable TBDMS (tert-butyldimethylsilyl) derivatization of hydroxyl, carboxyl, thiol and primary and secondary amines with typical yields over 96%.
TMSI (N-Trimethylsilylimisazole) The strongest silylator for carbohydrates and steroids Reacts quickly and smoothly with hydroxyls and carboxylic acids, but not with amines.
HMDS (Hexamethyldisilazane) The popular choice for silylation of sugars and related substances to extend the practical range of GC and improve chromatographic results.
MOX (Methoxamine) Useful for preparing oximes of steroids and ketoacids prior to silylation
Tri-Sil HTP (HMDS:TMCS:Pyridine) Reagent-catalyst mixture for one-step derivatization of carbohydrates, phenols, steroids, sterols, organic acids, alcohols and some amines
Tri-Sil BP (BSA:Pyridine) Derivatizes alcohols, phenols, organic acids, aromatic amides and amines
Tri-Sil TBT (TMSI:BSA:TMCS) A catalyzed silylation reagent formulation containing three parts TMSI, three parts BSA and two parts TMCS
Tri-Sil TP (TMSI:Pyridine) Derivatizes hydroxyl compounds, particularly carbohydrates
TMCS (Trimethylchlorosilane) An excellent catalyst for difficult-to-silylate compounds. Excellent adjunct for forming trimethylsilyl ethers for GC determinations.
Silylation Grade Solvents Manufactured for the exacting needs of silylation and other sensitive derivatization reactions.

Acylation Reagents

Converts compounds that contain active hydrogens through the action of a carboxylic acid or a carboxylic acid derivative
Pentafluoropropanol Purified for GC-MS applications, The addition of fluorine greatly enhances the sensitivity of certain detectors.
MBTFA (N-Methyl-bis(trifluoroacetamide) For trifluoroacylating primary and secondary amines, hydroxyl and thiol groups and carbohydrates.
Perfluoroacylimidazoles (HFBI and TFAI) Offer effective acylation of hydroxyl groups and primary and secondary amines.
Perfluoro Acid Anhydrides (TFAA, PFAA and HFAA) Highly purified for optimal preparation of fluoracyl derivatives.

Alkylation Reagents

Substitution of an active hydrogen by an aliphatic or aliphatic-aromatic (benzyl) group for modifying compounds containing acidic hydrogens, such as carboxylic acids and phenols.
BF3-Methanol Provides convenient, fast and quantitative esterification of fatty acids.
Pentafluorobenzyl Bromide (PFBBr) For electron capture GC analysis of carboxyl acids, phenols and sulfonamides.
Methylate (DMFDMA) For easy, effective preparation of methyl esters from fatty acids and amino acids.
MethElute (TMPAH) Alylation Reagent Provides accurate sensitive on-column methylation.

Siliconizing Fluids

Coat various lab consumables with an inert, water-repellent film.
Water-Soluble Siliconizing Fluid Attaches the silane polymer, octadecyltrialkosilane, to make the surface inert or polymerizes to create an inert film.
Hydrocarbon-Soluble Siliconizing Fluid Attaches a short-chain silane polymer to make the surface inert or polymerizes to create an inert film.
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