Collagens are fibrous extracellular matrix proteins that together comprise the most abundant protein in the human body. They have high tensile strength and are major components of connective tissues like tendons, cartilage, ligaments, and skin. Collagens are also plentiful in bone, intervertebral discs, corneas, blood vessels, tooth dentin, and gut tissue.

Many collagens are involved in cell adhesion and help maintain tissue architecture and function. As a structural support, collagens help form the interstitial matrix and the basement membrane that make up the extracellular matrix. All collagens have a triple helix domain, but the collagen family includes many chain types, each with multiple isoforms. Types I, II, III, IV, and V are the most common, with type I the most abundant of all. Types I, II, III, and V are fibril forming interstitial collagens, while type IV collagens form the basal lamina, a layer of the basement membrane. More specifically, type I collagens are found in bone, skin, tendons, and organs; type II collagens are the primary component of cartilage; type III collagens are the main component of reticular fibers; and type V collagens are found on the cell surface, in hair, and in the placenta.

Collagen antibodies have provided a useful means for studying the distribution of these connective tissue proteins. Collagen antibodies are also valuable in probing epithelial-mesenchymal interactions, tumorigenesis and the effect of carcinomas on the basement membrane, and the role of the basement membrane in development and epithelial differentiation. Quality Invitrogen collagen antibodies are available for a variety of research needs.

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Immunohistochemical analysis of collagen III in formalin-fixed, paraffin-embedded rat skin tissue. Sample was probed with a collagen III monoclonal antibody (Cat. No. MA1-22147) at a dilution of 1:4,000 and then subsequently detected using a biotin/avidin-peroxidase conjugate.

Immunofluorescent analysis of collagen I alpha 1 propeptide in formaldehyde-fixed, paraffin-embedded fibrotic mouse lung tissue. A specific collagen I alpha 1 propeptide polyclonal antibody (Cat. No. PA5-35379) provided specific staining of collagen I molecules (red) that are still associated with the cells in which they were synthesized. Prior to staining, antigen retrieval was performed by incubating tissues for 20 minutes at 90°C in 10 mM sodium citrate (pH 6.0)/0.1 % Tween 20.

Annotated product references

Cat. No. MA1-22147 was used in immunohistochemistry to investigate the effect of low-level laser phototherapy on collagen remodeling during skeletal muscle repair. Lasers in medical science (Nov 2011; 26: 803) "Phototherapy with low-level laser affects the remodeling of types I and III collagen in skeletal muscle repair." de Souza TO,Mesquita DA,Ferrari RA,Dos Santos Pinto D,Correa L,Bussadori SK,Fernandes KP,Martins MD

Cat. No. MA1-22147 was used in western blot to investigate the efficacy of angiotensin-(1-7) for the treatment of spontaneous hypertension-induced cardiac remodeling and interstitial fibrosis. Toxicology letters (Nov 2010; 199: 173) "Angiotensin-(1-7) ameliorates myocardial remodeling and interstitial fibrosis in spontaneous hypertension: role of MMPs/TIMPs." Pei Z,Meng R,Li G,Yan G,Xu C,Zhuang Z,Ren J,Wu Z