A fast, simple, and precise method to perform gene edits

The transformative CRISPR-Cas9 technology is revolutionizing the field of genome editing. Able to achieve highly flexible and precise targeting, the CRISPR-Cas9 system can be modified and redirected to become a powerful tool for genome editing in broad applications. 

From designing, synthesizing, and validating your CRISPR process, we offer end-to-end services to support every step in genome editing. Our dedicated R&D scientists and technical support specialists are ready to partner with you and your colleagues to accelerate your research program.

Contact us

Available CRISPR-Cas9 engineering services

Choose from a number of services to customize your genome engineering project.  Also see our custom CRISPR cell line engineering services.

Table 1. Recommended gene editing technology formats based on applications and outcomes

Format CRISPR-Cas9 protein (RNP) CRISPR-Cas9 mRNA TAL mRNA CRISPR-Cas9 plasmids
 Product IVT gRNA  + Cas9 nuclease IVT gRNA + Cas9 mRNA TAL mRNA  AiO CRISPR-Cas9 (OFP/CD4) plasmids
Application
  • Editing a single-nucleotide polymorphism (SNP) with ssODN (single-stranded DNA oligonucleotides)
  • Knockout (KO)
  • Deletions
  • Editing a SNP with ss-oligo
  • KO
  • Deletions
  • Editing a SNP with ss-Oligo
  • Knockin (KI) with double stranded DNA (Ex: fluorescent tags to gene)
Features
  • Footprint-free editing
  • No promoter constraint
  • Controlled dosage
  • Microinjection ready
  • Highest peak activity 
  • Stable RNP complex
  • Fast turnover
  • Footprint-free editing
  • No promoter constraint
  • Controled dosage
  • Microinjection ready
  • Non PAM-dependent
  • Reporter-based OFP or CD4 enrichment
Benefits
  • Delivery of RNP is generally most efficient (higher cleavage; minimal off-target)
  • Flexibility in multiplex assays
  • Minimal toxicity
  • Reduces off-target effects
  • Flexibility in multiplex assays
  • Boost % homology directed repair (HDR)*
  • Increase cleavage efficiency with enrichment
Cell type Most cell types Most adherent cell lines Most cell types Easy to transfect cells
Transfection reagent Neon/CRISPRMax MessengerMax MessengerMAX LF3K/Neon
*Note: improve % HDR when cut site is less than 10 bp from SNP

We’ve developed robust algorithms that allow us to generate the most optimal CRISPR-Cas9 gRNA designs, taking into consideration potential off-target effects. 

Based on our experience, we recommend that you design and test at least 3 gRNAs and choose the design that provides you with the highest on-target efficiency and minimal off-target effects. We offer a validation service where we design and test at least 3 designs and then provide you with the best-performing gRNAs.

Additional CRISPR-Cas9 validation services

  • Validate CRISPR-Cas9 gRNA design efficacy via genome cleavage detection (GCD) assay
  • Validate CRISPR-Cas9 gRNA design efficacy via on-target next-generation sequencing (NGS) analysis
  • Verify lack of off-target effects by NGS using Ion AmpliSeq™ technology

Need assistance with CRISPR gRNA design?

Our CRISPR Search & Design tool allows you to search our database of >600,000 predesigned CRISPR gRNAs in human and mouse genes or analyze your sequence of interest for de novo gRNA designs using our proprietary algorithms. Up to 25 gRNA sequences per gene are provided with recommendations based on potential off-target effects for each CRISPR sequence.

Start designing today ›

Let’s work together to get your genome engineering project started

Discover how our off-the-shelf product portfolio and outsourced services can meet your needs. Contact our dedicated technical support team with any questions, or get started on your services project today: