CytoTune®-iPS Sendai Reprogramming Kit (Gibco™)

The CytoTune™ -iPS Sendai Reprogramming Kit is a high efficiency, integration- free, easy-to-use somatic cell reprogramming kit used to generate induced pluripotent stem cells (iPSCs). This kit utilizes Sendai virus particles of the four Yamanaka factors, which have been shown to ...

Specifications

Titer
Lot-Dependent (≥3 x 10^7 cell infectious units⁄ml)
Delivery Method
Sendai
Sub-Cellular Localization
Cytoplasm
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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CytoTune™-iPS 2.0 Sendai Reprogramming Kit (Invitrogen™)

The CytoTune™-iPS 2.0 Sendai Reprogramming Kit improves upon the well-referenced CytoTune™-iPS Sendai Reprogramming Kit. Improvements in the 2.0 system include increased efficiency, lower cytotoxicity, and faster clearance in the generation of induced pluripotent stem cells. This ...

Specifications

Promoter
Proprietary
Delivery Method
Sendai virus
Sub-Cellular Localization
Cytoplasm
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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CytoTune™ EmGFP Sendai Fluorescence Reporter (Invitrogen™)

The CytoTune™ EmGFP Sendai Fluorescence Reporter helps you determine whether the Sendai particles that are used in all CytoTune™-iPS Sendai reprogramming kits can transduce into a given cell type before you advance to one of these kits. This vector expresses the next-generation A ...

Specifications

Promoter
Proprietary
Reporter
GFP (EmGFP)
Delivery Method
Sendai virus
Sub-Cellular Localization
Cytoplasm
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A16519
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Essential 6™ Medium (Gibco™)

Essential 6™ Medium is a feeder-free and xeno-free medium that supports the reprogramming of somatic cells and differentiation of human pluripotent stem cells (PSCs). The formulation is based on a medium originally developed by Guokai Chen et al. (1) in the laboratory of James Th ...

Specifications

Cell Type
Stem Cells (iPS - Induced Pluripotent Stem)
Form
Liquid
Species
Human
Serum Level
Serum-Free
Culture Type
Feeder Cell-Free
Classification
Xeno-Free
Volume (Metric)
500 ml
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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TaqMan® iPSC Sendai Detection Kit (Applied Biosystems™)

The TaqMan® iPSC Sendai Detection Kit contains 5 TaqMan® assays for detecting the presence and determining the levels of Sendai virus and exogenous transcription factors (OCT3/4, SOX2, KLF4, and cMyc) delivered to cells through use of the CytoTune™-iPS Reprogramming Kit. The assa ...

Specifications

PCR Method
2-Step qRT-PCR
Detection Method
Primer-Probe Detection
Primer-Probe Compatibility
TaqMan®
Reaction Speed
Fast
For use with (Equipment)
7900HT System
OpenArray®
StepOnePlus™, Standard Mode
7500 Fast System
7900HT Fast System
StepOnePlus™, Fast Mode
StepOne™, Standard Mode
StepOne™, Fast Mode
7500 System
Sample Type (General)
RNA
Technique
FRET
Sensitivity
Down to 10 copies
Label or Dye
FAM™
Target Specificity
Target-Specific
Sample Type (Specific)
Total RNA
Viral RNA

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StemPro® CD34+ Cell Kit

StemPro® CD34+ cells are pooled Human Hematopoietic Progenitor cells (HPCs) derived from the umbilical cord blood of mixed donors. Along with 0.5×106 pooled donor cord blood-derived CD34+ cells, this kit includes StemPro®-34 SFM basal liquid medium and frozen StemPro®-34 Nutrient ...

Specifications

Age
Newborn (14 days or less)
Form
Cryopreserved
Species
Human
Cell Type
Stem Cells (Hematopoietic)
Culture Type
Suspension Cell Culture
Classification
Serum-Free
Number of Cells
0.5 x 106
Donor Attributes
Normal
Media Recommendation
StemPro®-34 SFM Media
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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PSC Neural Induction Medium (Gibco™)

Gibco® PSC Neural Induction Medium is a serum-free medium that provides high efficiency neural induction of human pluripotent stems cells (PSCs) in only 7 days. Unlike existing methodologies, use of PSC Neural Induction Medium does not require the intermediary step of embryoid bo ...

Specifications

Cell Type
Stem Cells (Neural)
Stem Cells
Stem Cells (Embryonic)
Stem Cells (Human)
Stem Cells (iPS - Induced Pluripotent Stem)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

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