Genomics and proteomics search results

FRAP1 (mTOR) Recombinant Human Protein

FRAP1 (mTOR) is a serine⁄threonine kinase that plays a critical role in cellular growth and proliferation. Perturbations in the mTOR⁄PI3-kinase⁄AKT pathway are associated with numerous forms of cancer. FRAP1 is also the target of rapamycin and its analogues, which are currently ...

Specifications

Species
Human
Target Gene
FK506 binding protein 12-rapamycin associated protein 1
Gene Aliases
FRAP1 (mTOR)
Kinase Group
Ser⁄Thr Kinases: Atypical
Protein Family
Kinases & Inhibitors
Protein Subtype
Serine⁄Threonine Kinases
Accession Number
NP_004949.1
Molecular Weight
163.9 kDa
Research Category
Signal Transduction
Oncology
System Compatibility
LanthaScreen® Substrate
Z'-LYTE™
LanthaScreen® Antibody
Validated Application
Kinase Assay
Protein Length or Portion (a.a.)
aa 1360-2549
Protein Tag or Fusion
GST Tag
Expression
Baculovirus
Kinase Family
PIKK Kinase Family
Protein Form
Catalytic
Recombinant
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV4753
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FRAP1 (mTOR) Assay Buffer

1X Assay Buffer for use with the LanthaScreen® FRAP1 (mTOR) assays. ...

Specifications

Form
Liquid
Shipping Condition
Approved for shipment at Room Temperature or on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV4794
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Pierce™ mTOR Antibody (7HCLC), Abfinity™ Rabbit Oligoclonal (Novex™)

ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression v ...

Specifications

Reactivity
Human
Validated Application
Flow Cytometry
Western Blot
Immunocytochemistry
ELISA
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Oligoclonal
Clone ID
7HCLC
Antibody Fragment
Whole Antibody
Target Gene
mechanistic target of rapamycin (serine/threonine kinase)
Gene ID
2475
Gene Symbol
MTOR
Accession Number
P42345
Gene Aliases
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Label or Dye
Unconjugated
Concentration
0.5 mg⁄ml
Form
Liquid
Protein Family
Kinases & Inhibitors
Buffer
PBS (Phosphate Buffered Saline)
Research Category
Cell Cycle & Proliferation
Other Additives
Sodium Azide Preservative
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

710408
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Pierce™ mTOR Antibody (7H13L18), Abfinity™ Rabbit Monoclonal (Novex™)

ABfinity™ recombinant antibodies are rabbit monoclonal antibodies, unmatched for producing superior results. ABfinity™ antibodies are developed by immunizing animals, screening for functionality, cloning the immunogen-specific antibody genes into high-level mammalian expression v ...

Specifications

Reactivity
Human
Validated Application
Flow Cytometry
Western Blot
Immunocytochemistry
ELISA
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Monoclonal
Clone ID
7H13L18
Antibody Fragment
Whole Antibody
Target Gene
mechanistic target of rapamycin (serine/threonine kinase)
Gene ID
2475
Gene Symbol
MTOR
Accession Number
P42345
Gene Aliases
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Label or Dye
Unconjugated
Concentration
0.5 mg⁄ml
Form
Liquid
Protein Family
Kinases & Inhibitors
Buffer
PBS (Phosphate Buffered Saline)
Research Category
Signal Transduction
Other Additives
Sodium Azide Preservative
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

701483
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Novex™ mTOR Antibody (215Q18) (Novex™)

Mammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3-kinase and Akt/PKB signaling, resulting in phosphorylation of 4EBP1, and initiation of mRNA translat ...

Specifications

Reactivity
Human
Mouse
Rat
Validated Application
Western Blot
Host
Mouse
Clonality
Monoclonal
Clone ID
215Q18
Target Gene
mTOR
Gene ID
2475, 56717, 56718
Label or Dye
Unconjugated
Purification
Affinity Purified
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

AHO1232
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Rapamycin (Novex™)

Rapamycin is a member of a family of macrolide immunosuppressants that bind to and inhibit the FK506 binding protein (FKBP) proline rotamase. ...

Specifications

Form
Solid
Gene ID
2475
Gene Symbol
MTOR
Gene Aliases
FRAP, FRAP1, FRAP2, RAFT1, RAPT1
Molecular Weight
914.2 Da
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PHZ1235
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Pierce™ mTOR Antibody (Thermo Scientific™)

Western blot analysis of PA1-188 detects mTOR at ~250 kDa in human and non-human primate cells, slightly below the theoretical MW of ~280kDa. Using a Tris-Acetate SDS gel for high molecular weight protein separation, and a nitrocellulose membrane for protein transfer are necessar ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA1188
2a6e1361071c65ea9ef5a6de9ed5e4b4_CmgtBuyProduct

Pierce™ mTOR Antibody (Thermo Scientific™)

3-8% Tris-Acetate Gel is recommended for Western blot. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
99c36fbbe49acfd132d636bfea403504_CmgtBuyProduct

LanthaScreen® Akt HEK 293E Cell Line

LanthaScreen® GFP Cellular Assays allow for the analysis of post-translational modifications for a number of target proteins in an live-cell context. The PI3K⁄AKT pathway mediates signals for cell growth, cell survival, transcription, translation, and glucose uptake. Because of t ...

Specifications

System
LanthaScreen®
Readout
TR-FRET
Reporter
GFP (EmGFP)
Cell Line
293E (HEK)
Cell State
Dividing Cells
Antagonists
PI-103
LY294002
Gene Symbol
AKT1
Primary Agonist
IGF-1
Detection Method
Fluorescent
Druggable Target
Kinases
Signaling Pathway
Optimal Response
IGF-1 (EC50 = 0.42 µg/ml)
Alternate Agonists
Insulin
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

K1615
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Novex™ Phospho-mTOR / FRAP pSer2448 Antibody (Novex™)

Mammalian target of rapamycin (mTOR) is a serine/threonine kinase that plays a key role in cell growth, cell proliferation, and protein synthesis. mTOR mediates phosphoinositide 3-kinase and Akt/PKB signaling, resulting in phosphorylation of 4EBP1, and initiation of mRNA translat ...

Specifications

Reactivity
Human
Mouse
Validated Application
Western Blot
Immunofluorescence
Host
Rabbit
SubClass (Isotype)
IgG
Clonality
Polyclonal
Target Gene
mTOR/FRAP [pS2448]
Gene ID
2475
Label or Dye
Unconjugated
Target Modification State
Phospho
Purification
Purified
Protein Family
Kinases & Inhibitors
Research Category
Signal Transduction
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

441125G
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Pierce™ TOR Antibody (Thermo Scientific™)

A suggested positive control is L1210 cell lysate. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA520123
7f331bc28c3e25b0ed126c401a515fcd_CmgtBuyProduct

Pierce™ mTOR Antibody (Thermo Scientific™)

It is not recommended to aliquot this antibody. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA517780
c1762068e8e8c7694d272f49bf823547_CmgtBuyProduct

Pierce™ mTOR Antibody (Thermo Scientific™)

This antibody is predicted to react with mouse and rat based on sequence homology. ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
PA513263
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GFP-4E-BP1

4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor). ...

Specifications

Substrate
4E-BP1
Label or Dye
GFP (EmGFP)
Target Enzyme
Serine⁄Threonine Kinases
Substrate Type
Kinase Substrate
Detection Method
Fluorescent
Molecular Weight
45600 Da
Validated Kinases
FRAP1 (mTOR)
FRAP1 (mTOR)
Substrate Properties
Protein-Based Substrate
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

PV4759
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