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GeneArt® Seamless Cloning and Assembly Kit (Invitrogen™)

The GeneArt® Seamless Cloning and Assembly Kit enables the simultaneous and directional cloning of 1 to 4 PCR fragments, consisting of any sequence, into any linearized vector, in a single 30-minute room temperature reaction. The kit contains everything required for the assembly ...

Specifications

Maximum Size
13 kb total (vector plus all inserts)
Workflow Step
DNA Assembly
Cloning Method
Seamless Cloning
Number of Fragments
Up to 4 Fragments
Bacterial or Yeast Strain
TOP10
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A13288
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GeneArt® Site-Directed Mutagenesis System (Invitrogen™)

The GeneArt® Site-Directed Mutagenesis System provides a state-of-the-art, simple, convenient, and highly efficient means to generate mutations on a target construct in vitro in less than three hours. This system replaces the popular GeneTailor™ Site-Directed Mutagenesis System, ...

Specifications

Mutagenesis Capability
Single-Site Mutagenesis
Plasmid Type
Plasmids up to 14 kb
Mutation Type
Deletions
Substitutions
Insertions
Mutagenesis Efficiency
90%
Bacterial or Yeast Strain
DH5α™
Mutagenized Region Length (up to)
12 nucleotides
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A13282
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GeneArt® High-Order Genetic Assembly System (Invitrogen™)

The GeneArt® High-Order Genetic Assembly System is a highly efficient kit for the simultaneous and seamless assembly of up to 10 DNA fragments, totaling up to 110 Kbp in length, into any vector. The system relies on yeast’s ability to take up and recombine DNA fragments with high ...

Specifications

Maximum Size
110 kb total (vector plus all inserts)
Workflow Step
DNA Assembly
Cloning Method
Seamless Cloning
Number of Fragments
Up to 10 Fragments
Bacterial or Yeast Strain
TOP10
MaV203
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A13285
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GeneArt® Chlamydomonas Engineering Kit (Invitrogen™)

The GeneArt® Chlamydomonas Engineering Kit is the first commercially available genetic modification and expression system for the eukaryotic microalgae, Chlamydomonas reinhardtii. The GeneArt® Chlamydomonas Engineering Kit combines optimized cloning and expression vectors, frozen ...

Specifications

Promoter
Hsp70A-RbcS2
Cell Line
Chlamydomonas
Expression
Algae
Key Function
Algal Engineering
Delivery Method
Transformation
Protein Tag or Fusion
Untagged
Selection Agent (Eukaryotic)
Hygromycin
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A14258
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GeneArt® High-Order Genetic Assembly Systems, with yeast growth media (Invitrogen™)

The GeneArt® High-Order Genetic Assembly System is a highly efficient kit for the simultaneous and seamless assembly of up to 10 DNA fragments, totaling up to 110 Kbp in length, into any vector. The system relies on yeast’s ability to take up and recombine DNA fragments with high ...

Specifications

Maximum Size
110 kb total (vector plus all inserts)
Workflow Step
DNA Assembly
Cloning Method
Seamless Cloning
Number of Fragments
Up to 10 Fragments
Bacterial or Yeast Strain
TOP10
MaV203
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A13286
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GeneArt® Chlamydomonas TOPO® Engineering Kit (Invitrogen™)

The GeneArt® Chlamydomonas TOPO® Engineering Kit is the first commercially available genetic modification and expression system for the eukaryotic microalgae, Chlamydomonas reinhardtii. The GeneArt® Chlamydomonas TOPO® Engineering Kit combines TOPO® cloning and expression vectors ...

Specifications

Promoter
Hsp70A-RbcS2
Cell Line
Chlamydomonas
Expression
Algae
Key Function
Algal Engineering
Delivery Method
Transformation
Protein Tag or Fusion
Untagged
Selection Agent (Eukaryotic)
Hygromycin
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A14260
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GeneArt® Chlamydomonas TOPO® Engineering Kit, with 6 L media (Invitrogen™)

The GeneArt® Chlamydomonas TOPO® Engineering Kit is the first commercially available genetic modification and expression system for the eukaryotic microalgae, Chlamydomonas reinhardtii. The GeneArt® Chlamydomonas TOPO® Engineering Kit with media combines TOPO® cloning and express ...

Specifications

Promoter
Hsp70A-RbcS2
Cell Line
Chlamydomonas
Expression
Algae
Key Function
Algal Engineering
Delivery Method
Transformation
Protein Tag or Fusion
Untagged
Selection Agent (Eukaryotic)
Hygromycin
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A14264
bce6f6f9fd6958bd2bf910e41ab94199_CmgtBuyProduct

MTO GeneArt® Eng KO Cell Model - Small Commercial (<250 Employees) (Invitrogen™)

Thermo Fisher Scientific is pleased to introduce GeneArt Engineered Cell Models, a collection of thousands of diploid and haploid cell lines containing defined genomic modifications. ...

Specifications

A30305
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GeneArt™ Precision gRNA Synthesis Kit (Invitrogen™)

GeneArt™ Precision gRNA Synthesis Kit is a complete system for rapid synthesis of guide RNA (gRNA) ready to complex with GeneArt™ Platinum™ Cas9 Nuclease for transfection-ready Cas9 protein/gRNA ribonucleoprotein (Cas9 RNP). This Cas9 RNP format, with our Platinum Cas9 nuclease, ...

Specifications

Starting Material
DNA
Promoter
T7
RNA Yield
>10 µg at >200 ng/µL of gRNA
Final Product
RNA
Shipping Condition
Approved for shipment at Room Temperature or on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
A29377
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GeneArt™ Platinum™ Cas9 Nuclease (3 µg/µL) (Invitrogen™)

GeneArt™ Platinum™ Cas9 Nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein purified from E. coli that can be used for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of t ...

Specifications

Concentration
3 µg/µL
Research Category
DNA Replication & Repair
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
B25641
427912f30a32f60dea5652d6fd96c35c_CmgtBuyProduct

GeneArt™ Platinum™ Cas9 Nuclease (1 µg/µL) (Invitrogen™)

GeneArt™ Platinum™ Cas9 Nuclease is the recombinant Streptococcus pyogenes Cas9 (wt) protein purified from E. coli that can be used for genome editing with CRISPR technology. Cas9 protein forms a very stable ribonucleoprotein (RNP) complex with the guide RNA (gRNA) component of t ...

Specifications

Concentration
1 µg/µL
Research Category
DNA Replication & Repair
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
B25640
47c29eb4023189b91f2bd6876fe45f5e_CmgtBuyProduct