BigDye® Terminator v3.1 Cycle Sequencing Kit (Applied Biosystems™)

The BigDye® Terminator v3.1 Cycle Sequencing Kit's robust, highly flexible chemistry is ideal for de novo sequencing, resequencing, and finishing with PCR product, Plasmid, Fosmid, and BAC templates.
• Improve the quality of your results for a wide range of sequencing application ...

Specifications

For use with (Equipment)
GeneAmp® 9700
Veriti® Thermal Cycler
3500xL Genetic Analyzer
3730 DNA Analyzer
3130 Genetic Analyzer
310 Genetic Analyzer
3130xl Genetic Analyzer
3730xl DNA Analyzer
3500 Genetic Analyzer
Recommended Applications
AT-Rich Sequencing (>65%)
Comparative Sequencing (Germline Mutations - 50:50)
GC-Rich Sequencing (>65%)
De Novo Sequencing - High Throughput (cDNA)
Mixed-Base Detection
Resequencing
Long-Read Sequencing
GT-Rich or Difficult Template Sequencing
Template Compatibility
BAC DNA
Lambda DNA
Genomic DNA (Bacterial)
PCR Amplicons
PCR Amplicons (50:50 Heterozygotes)
Rolling Circle Amplified Products
Plasmid DNA (≤15Kb)
Fosmids
Single Stranded DNA
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4337454
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3500/3500xL Sequencing Standards, BigDye® Terminator v3.1 (Applied Biosystems™)

The BigDye® Terminator v3.1 sequencing standard kit contains four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration⁄performance check and⁄or one control sequencing run on the Appli ...

Specifications

Label or Dye
TAMRA™
Rhodamine 6G
Rhodamine 110
ROX™
For use with (Equipment)
3500xL Genetic Analyzer
3500 Genetic Analyzer
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4404312
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BigDye® Terminator v3.1 Cycle Sequencing Reagents-CG (Applied Biosystems™)

The BigDye Terminator v3.1 Cycle Sequencing Reagents-CG are General Purpose Reagents that provide the required components for cycle sequencing reactions in a pre-mixed format. You need only provide the template and template-specific primer. These reagents are suitable for perform ...

Specifications

For use with (Equipment)
3730 DNA Analyzer
3500 Genetic Analyzer
3130 Genetic Analyzer
Recommended Applications
Sequencing
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4486481
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BigDye® Terminator v3.1 Sequencing Standard-CG, 3730 Series (Applied Biosystems™)

The BigDye® Terminator v3.1 Sequencing Standard-CG is a General Purpose Reagent that consists of four tubes, each of which contains enough hsp69 DNA of a known sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration/performance check and/or one control ...

Specifications

Label or Dye
Dye Set Z
For use with (Equipment)
3730 DNA Analyzer
Regulatory Statement
For Laboratory Use

Related Applications

4486194
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BigDye® Terminator v3.1 Sequencing Standard-CG, 3500 Series (Applied Biosystems™)

The BigDye® Terminator v3.1 Sequencing Standard-CG is a General Purpose Reagent that consists of four tubes, each of which contains enough DNA of a known 1200 base pair sequence prepared with BigDye® Terminator v3.1 to perform one spectral calibration?performance check and?or one ...

Specifications

Label or Dye
Dye Set Z
For use with (Equipment)
3500 Genetic Analyzer
Regulatory Statement
For Laboratory Use

Related Applications

4486193
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BigDye® Terminator v1.1 & v3.1 5X Sequencing Buffer (Applied Biosystems™)

This sequencing buffer is optimized for use with BigDye® Terminator v1.1 and v3.1 Cycle Sequencing Kits. ...

Specifications

Form
Liquid
Buffer Type
Sequencing Buffer
Concentrated
5 X
For use with (Equipment)
GeneAmp® 9700
Veriti® Thermal Cycler
3500xL Genetic Analyzer
3130xl Genetic Analyzer
3730xl DNA Analyzer
3500 Genetic Analyzer
3730 DNA Analyzer
Recommended Applications
Standard Sequencing
Shipping Condition
Room Temperature
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4336699
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3730/3730xl DNA Analyzer Sequencing Standards, BigDye® Terminator v3.1 (Applied Biosystems™)

This kit contains four tubes of hsp69 standard sequence used for instrument and spectral calibrations. This material is used with the 3730, and 3730xl DNA Analyzers. ...

Specifications

For use with (Equipment)
3730 DNA Analyzer
3730xl DNA Analyzer
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4336943
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31xx Matrix Standards Kit, BigDye® Terminator v3.1 (Applied Biosystems™)

This kit contains standards used to generate a spectral on the 3100, 3100-Avant™, 3130, 3130xl, 3500 and 3500xl Genetic Analyzers. ...

Specifications

Label or Dye
TAMRA™
Rhodamine 6G
Rhodamine 110
ROX™
For use with (Equipment)
3500xL Genetic Analyzer
3100 Genetic Analyzer
3500 Genetic Analyzer
3130 Genetic Analyzer
3130xl Genetic Analyzer
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4336974
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310/31xx Genetic Analyzer Sequencing Standards, BigDye® Terminator v3.1 (Applied Biosystems™)

This kit contains four tubes of hsp69 standard sequence used for instrument and spectral calibrations. This material is used with the 310, 3100, 3100-Avant™, 3130 and 3130xl Genetic Analyzers. ...

Specifications

Label or Dye
TAMRA™
Rhodamine 6G
Rhodamine 110
ROX™
For use with (Equipment)
3130xl Genetic Analyzer
310 Genetic Analyzer
3100 Genetic Analyzer
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4336935
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310 Genetic Analyzer Matrix Standards, BigDye® Terminator v3.1 (Applied Biosystems™)

This kit contains a set of four standards that can be used to generate a matrix file for the 310 Genetic Analyzer. Each tube contains a different fluorescent dye in solution, along with blue loading dye. ...

Specifications

Label or Dye
TAMRA™
Rhodamine 6G
Rhodamine 110
ROX™
For use with (Equipment)
310 Genetic Analyzer
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4336948
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3730xl DNA Analyzer 96-Capillary Array, 50 cm (Applied Biosystems™)

This is a Capillary Array for the Applied Biosystems® 3730xl DNA Analyzer. Each array contains 96 capillaries, 50 cm long. Used for standard sequencing. ...

Specifications

Array Format
Capillary Array
Capillary Length
50 cm
Number of Capillaries
96 Capillaries
For use with (Equipment)
3730xl DNA Analyzer
Recommended Applications
Sequencing
Shipping Condition
Room Temperature
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4331246
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Glass Syringe, 1.0 mL (Applied Biosystems™)

This Glass Syringe is suitable for filling 310 Genetic Analyzer capillaries with POP-4® Performance Optimized Polymer, POP-6™ Performance Optimized Polymer, and GeneScan™ polymer. ...

Specifications

Type
Syringe
For use with (Equipment)
310 Genetic Analyzer
Shipping Condition
Room Temperature
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

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POP-7™ Polymer for 3730/3730xl DNA Analyzers (Applied Biosystems™)

The POP-7™ Polymer is a separation matrix for the 3730 and 3730xl DNA Analyzers. POP-7™ Polymer is customized for DNA sequencing and fragment analysis. Not suitable for use with the 310 and 3100 series genetic analyzers. It causes syringe failure and premature array degradation. ...

Specifications

Accuracy
98.5% up to 700 bases (for 36 cm capillary)
98.5% up to 900 bases (for 50 cm capillary)
Run Time
2 hrs for 50 cm capillary (long read seq.)
1 hr for 36 cm capillary (std. seq.)
For use with (Equipment)
3730 DNA Analyzer
Recommended Applications
Fragment Analysis (Denaturing)
Standard Sequencing
On-Instrument Life⁄Usage
Stability on instrument 7 days
Up to 12,000 samples/28 ml
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4335611
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3130xl/3100 Genetic Analyzer 16-Capillary Array, 50cm (Applied Biosystems™)

This is a 50 cm 16-capillary array (internally uncoated) for the 3130xl and 3100 Genetic Analyzer. Use for fragment analysis and sequencing applications. Capillary dimensions are 61 cm x 50 µm i.d. Usage per array is 100 runs. ...

Specifications

Run Time
165 min
Capillary Length
50 cm
Number of Capillaries
16 Capillaries
For use with (Equipment)
3130xl Genetic Analyzer
3100 Genetic Analyzer
Recommended Applications
Fragment Analysis
Sequencing
Shipping Condition
Room Temperature
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

4315930
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