Plasmid pBR322 (Invitrogen™)

Plasmid pBR322 (1) contains three unique restriction endonuclease recognition sites within the !-lactamase (AmpR) gene and eight unique sites within the TetR gene. The plasmid, purified from DH10B™ E. coli, also has 14 non-selectable unique restriction endonuclease recognition si ...

Specifications

Vector Type
pBR322
Cloning Method
Restriction Enzyme ⁄ MCS
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

15367014
245590935cf1f8c66e79b2da88a54117_CmgtBuyProduct

pBR322 Plasmid DNA (Thermo Scientific™)

Thermo Scientific pBR322 is one of the most commonly used E.coli cloning vectors. ...

Specifications

Vector Type
pBR322
Cloning Method
Restriction Enzyme ⁄ MCS
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

SD0041
c1bb7c90a1b66398ea3521e7964c4a8e_CmgtBuyProduct

pBR322 DNA/BsuRI (HaeIII) Marker (Thermo Scientific™)

Thermo Scientific pBR322 DNA/BsuRI (HaeIII) Marker is recommended for sizing and approximate quantification of small linear double-stranded DNA fragments in agarose and non-denaturing polyacrylamide gels. pBR322 DNA is digested to completion with the appropriate Thermo Scientific ...

Specifications

Gel Compatibility
Agarose Gels
Ready to Load
No
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

SM0271
dea4fc1d07f8811ddc319acd70c9fa65_CmgtBuyProduct

Gateway® pET-DEST42 Vector (Invitrogen™)

The Champion™ pET-DEST42 Gateway® destination vector is designed for rapid cloning with a Gateway® entry clone using lambda phage site-specific recombination and subsequent high-level prokaryotic expression controlled by the strong bacteriophage T7 promoter. Expression is induced ...

Specifications

Vector Type
pET
pDEST
Cloning Method
Gateway®
Promoter
T7 and lacO
Protein Tag or Fusion
His Tag (6x)
V5 Epitope Tag
Antibiotic Resistance (Bacterial)
Ampicillin (AmpR)
Inducing Agent
IPTG
Selection Agent (Eukaryotic)
None
Constitutive or Inducible System
Inducible
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

12276010
dcf059179471f166ab52527c90dd5f85_CmgtBuyProduct

BL21 Star™ (DE3)pLysS One Shot® Chemically Competent E. coli (Invitrogen™)

One Shot® BL21 Star™ (DE3) pLysS E. coli are chemically competent cells designed for applications that require high-level expression of non-toxic recombinant proteins from high copy number, T7 promoter-based expression systems (e.g., pRSET T7 vectors). One Shot® BL21 Star™ (DE3) ...

Specifications

Competent Cell Type
Chemically Competent
Species
E. coli
Format
One Shot
Blue-White Screening
No
Bacterial or Yeast Strain
BL21 Star™(DE3)pLysS
Reduces Recombination
No
Cloning Methylated DNA
No
Improves Plasmid Quality
No
Propagating ccdB Vectors
Not for ccdB vector propagation
Transformation Efficiency
> 1x10^8
F' Episome (to make ssDNA)
Lacks F' Episome
T1 Phage - Resistant (tonA)
No
High Throughput Compatibility
Not High Throughput-Compatible (Manual)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

C602003
87d92f5f43092460fff1b5a45cb6f2e4_CmgtBuyProduct

Dpn I (Invitrogen™)

5´-GmA↓TC-3´ 3´-CT↑mAG-5´

Important: Invitrogen restriction enzymes now come with Universal Buffers L, M, H, K, or T (+BSA) instead of REact® Buffers 1-8. Functionally, the enzymes are the same and have been validated for the recommended buffer. Please contact our technical servic ...

Specifications

Enzyme
Dpn I
Concentration
10 U⁄µl
Compatible Buffer
10X Buffer T
Methylation Sensitivity
Not dam methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes (Heat-Inactivatable)
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

15242019
2b227dd7db44d54f934ee76a57b1b3b5_CmgtBuyProduct

pUC18 DNA (Thermo Scientific™)

Thermo Scientific pUC18 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC19 vector except that it is arranged in opposite orientation. ...

Specifications

Vector Type
pUC18
Cloning Method
Restriction Enzyme ⁄ MCS
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

SD0051
985faf9a201c213693e86870c0a5d23b_CmgtBuyProduct

pUC19 DNA (Thermo Scientific™)

Thermo Scientific pUC19 vector is a small, high copy number, E. coli plasmid, 2686 bp in length. It contains identical multiple cloning site (MCS) as pUC18 vector except that it is arranged in opposite orientation. ...

Specifications

Vector Type
pUC19
Cloning Method
Restriction Enzyme ⁄ MCS
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

SD0061
fc6843f7ad64028c74b542b0735d5bbd_CmgtBuyProduct

SgeI (3 U/µL) (Thermo Scientific™)

5'  Cm5N  N  G  N9 3'
3'  G  N  N  C  N13 5'
SgeI cleaves DNA targets containing 5-methylcytosine on one or both DNA strands ...

Specifications

Enzyme
SgeI
Compatible Buffer
Unique Buffer (10x Buffer SgeI)
Methylation Sensitivity
Not CpG methylation-sensitive
Not dcm methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER2211
734032ec10bcbdd4e1cdd9c80967c305_CmgtBuyProduct

SalI (10 U/µL) (Thermo Scientific™)

5'  G ↓T  C  G  A  C   3'
3'  C  A  G  C  T ↑G   5'

Thermo Scientific SalI restriction enzyme recognizes G^TCGAC sites and cuts best at 37°C in O buffer. See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inacti ...

Specifications

Enzyme
Sal I
Compatible Buffer
10x Buffer O
Methylation Sensitivity
Not dam methylation-sensitive
Not dcm methylation-sensitive
CpG methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER0645
dafab6e5a61080665e9f66cac49d6350_CmgtBuyProduct

NheI (10 U/µL) (Thermo Scientific™)

5'  G ↓C  T  A  G  C   3'
3'  C  G  A  T  C ↑G   5'

Thermo Scientific NheI restriction enzyme recognizes G^CTAGC sites and cuts best at 37°C in Tango buffer (isoschizomers: AsuNHI, BmtI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for ...

Specifications

Enzyme
Nhe I
Compatible Buffer
10x Buffer Tango
Methylation Sensitivity
Not dam methylation-sensitive
Not dcm methylation-sensitive
CpG methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER0971
172cd3509fc148da13bf23b39539ca96_CmgtBuyProduct

Lsp1109I (BbvI) (5 U/µL) (Thermo Scientific™)

5'  G  C  A  G  C  N8 3'
3'  C  G  T  C  G  N12 5'

Thermo Scientific Lsp1109I (BbvI) restriction enzyme recognizes GCAGC(8/12)^ sites and cuts best at 37°C in its own unique buffer (isoschizomers: BbvI, BstV1I). See Reaction Conditions for Restriction Enzymes for a table of enzym ...

Specifications

Enzyme
Lsp1109I (BbvI)
Compatible Buffer
Unique Buffer (10x Buffer Lsp1109I (BbvI))
Methylation Sensitivity
Not dam methylation-sensitive
Not dcm methylation-sensitive
Not CpG methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER2071
b16aa3cbf6e76462411a7e6652804456_CmgtBuyProduct

CseI (HgaI) (5 U/µL) (Thermo Scientific™)

5'  G  A  C  G  C  N5 3'
3'  C  T  G  C  G  N10 5'

Thermo Scientific CseI (HgaI) restriction enzyme recognizes GACGC(5/10)^ sites and cuts best at 37°C in R buffer (isoschizomers: HgaI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions fo ...

Specifications

Enzyme
CseI (HgaI)
Compatible Buffer
10x Buffer R
Methylation Sensitivity
Not dam methylation-sensitive
Not dcm methylation-sensitive
CpG methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER1901
a501495eb2e6afc441c3f76431250160_CmgtBuyProduct

BseXI (BbvI) (3 U/µL) (Thermo Scientific™)

5'  G  C  A  G  C  N8 3'
3'  C  G  T  C  G  N12 5'

Thermo Scientific BseXI (BbvI) restriction enzyme recognizes GCAGC(8/12)^ sites and cuts best at 65°C in its own unique buffer (isoschizomers: BbvI, BstV1I). See Reaction Conditions for Restriction Enzymes for a table of enzyme a ...

Specifications

Enzyme
BseXI (BbvI)
Compatible Buffer
Unique Buffer (10x Buffer BseXI (BbvI))
Methylation Sensitivity
Not dam methylation-sensitive
Not dcm methylation-sensitive
Not CpG methylation-sensitive
Optimal Reaction Temperature
65° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER1451
4fbe21763a964bffb8da296bd0dbb2a2_CmgtBuyProduct

DpnI (10 U/µL) (Thermo Scientific™)

5'  G  Am6T  C   3'
3'  C  T    ↑Am6G   5'

Thermo Scientific DpnI restriction enzyme recognizes Gm6A^TC sites and cuts best at 37°C in Tango buffer (isoschizomers: MalI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestio ...

Specifications

Enzyme
Dpn I
Compatible Buffer
10x Buffer Tango
Methylation Sensitivity
Not CpG methylation-sensitive
dam methylation-sensitive
Not dcm methylation-sensitive
Optimal Reaction Temperature
37° C
Sensitive to Heat Inactivation
Yes
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

ER1705
cfd1a7990f37e683df37aced944ed5cc_CmgtBuyProduct