SuperScript® II Reverse Transcriptase (Invitrogen™)

SuperScript® II Reverse Transcriptase (RT) is an improved version of SuperScript® RT. It is a DNA polymerase that synthesizes a complementary DNA strand from single-stranded RNA, DNA, or from an RNA:DNA hybrid. This enzyme is genetically engineered by the introduction of point mu ...

Specifications

Optimal Reaction Temperature
42° C
Final Product Size(s)
12.3 kb or less
Reverse Transcriptase
SuperScript® II
Ribonuclease H Activity
Reduced
Form
Frozen
Format
Tube(s)
Template
ssDNA
ssRNA
RNA-DNA Hybrid
Sensitivity
Medium
Concentration
200 U⁄µl
Enzyme Function
RNA dependent DNA Polymerase
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

18064022
e057e25df05ae2eeb693676c28500d70_CmgtBuyProduct

SuperScript® III Reverse Transcriptase (Invitrogen™)

SuperScript® III Reverse Transcriptase (RT) is a proprietary mutant of SuperScript® II RT that is active at 50°C and has a half-life of 220 minutes, providing increased specificity with gene-specific primers (GSPs) and the highest cDNA yield of all RTs. It is ideal for RT-PCR of ...

Specifications

Optimal Reaction Temperature
50° C
Final Product Size(s)
12.3 kb or less
Reverse Transcriptase
SuperScript® III
Ribonuclease H Activity
Reduced
Form
Frozen
Format
Tube(s)
Template
ssRNA
Sensitivity
Medium
Concentration
200 U⁄µl
Enzyme Function
RNA dependent DNA Polymerase
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

18080093
407ccf2da2cc931bcb771edeb971ca4f_CmgtBuyProduct

SuperScript® Double-Stranded cDNA Synthesis Kit (Invitrogen™)

The SuperScript® Double-Stranded cDNA Synthesis Kit contains all of the reagents (except an oligo (dT)-containing primer) necessary to make high-quality, double-stranded cDNA from total RNA or poly A+-selected RNA (mRNA). The advanced capabilities of SuperScript® II Reverse Trans ...

Specifications

Reverse Transcriptase
SuperScript® II
Downstream Application
Microarray Analysis
Reaction Format
Separate Components
Sample Type (General)
RNA
Final Product
cDNA (Double-Stranded)
Labeling Method
Direct Labeling
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
Optimal Reaction Temperature
42° C

Related Applications

11917020
badf8d2984cb78cbe787b41cc405f452_CmgtBuyProduct

SuperScript® First-Strand Synthesis System for RT-PCR (Invitrogen™)

The SuperScript® First-Strand Synthesis System for RT-PCR is optimized to synthesize first-strand cDNA from purified poly(A)+ or total RNA. The system can be used with as little as 1 ng or as much as 5 µg of total RNA. After synthesis, the cDNA can be amplified with specific prim ...

Specifications

Reverse Transcriptase
SuperScript® II
Downstream Application
Reverse Transcriptase PCR (RT-PCR)
Reaction Format
Separate Components
Primer Type
Oligo dT Primers
Random Primers
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
Optimal Reaction Temperature
42° C

Related Applications

11904018
ec8649f75615ffb3fd1181acd62e7d55_CmgtBuyProduct

SuperScript® One-Step RT-PCR System with Platinum® Taq DNA Polymerase (Invitrogen™)

The SuperScript® One-Step RT-PCR System with Platinum® Taq DNA Polymerase is designed for the convenient, sensitive, and reproducible detection and analysis of RNA molecules by RT-PCR. Components for both cDNA synthesis and PCR are combined in a single tube, using gene-specific p ...

Specifications

PCR Method
1-Step RT-PCR
Reverse Transcriptase
SuperScript® II
Polymerase
Platinum® Taq DNA Polymerase
Hot Start
Built-In Hot Start
Sample Type (General)
RNA
Detection Method
Gel Electrophoresis
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
Final Product Size(s)
3 kb or less
Primer-Probe Compatibility
Unlabeled Oligos

Related Applications

10928042
b5ccd864d0e686ba0d09a465f0e7759e_CmgtBuyProduct

SuperScript® One-Step RT-PCR System for Long Templates (Invitrogen™)

The SuperScript® One-Step RT-PCR for Long Templates is designed for the convenient, sensitive, and reproducible detection and analysis of RNA molecules by one-step RT-PCR. Both cDNA synthesis and PCR are performed in a single tube using gene-specific primers and specific target R ...

Specifications

PCR Method
1-Step RT-PCR
Reverse Transcriptase
SuperScript® II
Polymerase
Platinum® Taq DNA Polymerase High Fidelity
Hot Start
Built-In Hot Start
Sample Type (General)
RNA
Detection Method
Gel Electrophoresis
Shipping Condition
Wet Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
Final Product Size(s)
9 kb or less
Primer-Probe Compatibility
Unlabeled Oligos

Related Applications

11922028
766c041ebc66bc4b68b8b822fbb78715_CmgtBuyProduct

SuperScript® One-Cycle cDNA Kit for use with Affymetrix® One-Cycle Assays (Invitrogen™)

The SuperScript® One-Cycle cDNA Kit for use with Affymetrix® One-Cycle Assays conveniently contains all of the reagents required for double-stranded cDNA synthesis within the Affymetrix GeneChip® workflow. The kit includes Invitrogen’s proprietary RNase H reduced activity SuperSc ...

Specifications

System
Affymetrix GeneChip®
Comments
For generating double-stranded cDNA
Label or Dye
Biotin
Final Product
cDNA (Double-Stranded)
Labeling Method
Direct Labeling
Labeling Target
cDNA
Detection Method
Biotin-Based
Includes Label or Dye
No
Sample Type (General)
RNA
Sample Type (Specific)
Total RNA
Poly(A+) RNA
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A10752030
280ba61d15eb7b9008a3011036c54a5a_CmgtBuyProduct

SuperScript® VILO™ cDNA Synthesis Kit (Invitrogen™)

The SuperScript® VILO™ cDNA Synthesis Kit provides the high-temperature capability of SuperScript® III Reverse Transcriptase (RT) in an optimized format for generating first-strand cDNA for use in real-time quantitative RT-PCR (qRT-PCR). VILO is an acronym for variable input, lin ...

Specifications

Reverse Transcriptase
SuperScript® III
Downstream Application
Real-Time Quantitative PCR (qPCR)
Reaction Format
SuperMix or Master Mix
Sample Type (General)
RNA
Primer Type
Random Primers
Shipping Condition
Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
Optimal Reaction Temperature
42° C

Related Applications

11754050
e934930a0706a25821b22965af41b835_CmgtBuyProduct

M-MLV Reverse Transcriptase Buffer (Invitrogen™)

M-MLV Reverse Transcriptase Buffer is supplied as 5X Buffer [250 mM Tris-HCl (pH 8.3), 375 mM KCl, 15 mM MgCl2] with 500 µl 0.1 M DTT. It is included with M-MLV, SuperScript®, SuperScript® II, and SuperScript® III reverse transcriptases. ...

Specifications

Form
Liquid
Format
Tube(s)
Shipping Condition
Approved for shipment on Wet or Dry Ice
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

18057018
90d6945751cf6c2ea20b84fb39ce5f92_CmgtBuyProduct

CloneMiner™ II cDNA Library Construction Kit (Invitrogen™)

The CloneMiner™ II cDNA Library Construction Kit is a second generation CloneMiner™ kit that enables rapid construction of highly representative cDNA libraries without restriction enzyme cloning. This innovative library construction technology combines SuperScript® III Reverse Tr ...

Specifications

Final Product
cDNA Library
Cloning Method
Gateway®
Reverse Transcriptase
SuperScript® III
Sample Type (General)
RNA
Bacterial or Yeast Strain
DH10B™
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

A11180
745305f2ce7ee4a30b24eb3b31d578e9_CmgtBuyProduct

SuperScript® Choice System for cDNA Synthesis (Invitrogen™)

The SuperScript® Choice System is designed for synthesis of double-stranded cDNA from a purified mRNA population. Advantages of the SuperScript® Choice system include: ...

Specifications

Reverse Transcriptase
SuperScript® II
Downstream Application
cDNA Library Construction
Reaction Format
Separate Components
Sample Type (General)
RNA
Final Product
cDNA (Second-Strand)
Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.
Optimal Reaction Temperature
42° C

Related Applications

18090019
29e532cb53f3dd9a396e75c18b9a263d_CmgtBuyProduct

SuperScript® Plasmid System with Gateway®Technology and ElectroMAX™ DH10B™ Competent Cells (Invitrogen™)

The SuperScript® Plasmid System is designed for synthesis of double-stranded cDNA from a purified mRNA population. The cDNA is suitable for directional cloning into the pSPORT1 vector or the pCMV ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

19625011
d372b8194829a0d053a1bcb5e9181773_CmgtBuyProduct

SuperScript® Plasmid System for cDNA Synthesis and Plasmid Cloning with Gateway™ Technology (Invitrogen™)

The SuperScript® Plasmid System is designed for synthesis of double-stranded cDNA from a purified mRNA population. The cDNA is suitable for directional cloning into the pSPORT1 vector or the pCMV ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

18248013
17676cf990988cbae50b816761c569d5_CmgtBuyProduct

3′ RACE System for Rapid Amplification of cDNA Ends (Invitrogen™)

3´ RACE System is suitable for rapid amplification of cDNA ends (RACE) (1-3) and anchored PCR between a defined point within mRNA and the 3´ poly(A) end (Figure 1). The system is useful for the amplification of rare messages for which little sequence information is available, and ...

Specifications

Regulatory Statement
For Research Use Only. Not for use in diagnostic procedures.

Related Applications

18373019
77426a74abfa6260d748549cc95fbc19_CmgtBuyProduct