Can I use the original Quant-iT™ Kits with the Qubit® Fluorometer?

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Answer

No, we do not recommend this. Some of the dyes in the original Quant-iT™ kits (those NOT listed as “for use with the Qubit® fluorometer”) are not compatible with the Qubit® Fluorometer. In addition, the new Quant-iT™ kits (labeled as “for use with the Qubit® Fluorometer”) have standards formulated to be more accurate with the Qubit® fluorometer. The Qubit® Fluorometer-compatible kits are also less expensive per assay if you are processing fewer than 20 samples at a time.

Answer Id: E5015

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550941aaebd324d688c53937dbb56f78_FAQ

Can I use the Quant-iT™ and Qubit™ Kits with other fluorometers?

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Answer

All Quant-iT™ and Qubit™ kits are compatible with all fluorometers and microplate readers that have the appropriate light sources and filters. You won't have access to the algorithm in the Qubit™ fluorometer for generating the standard curve provided by the instrument, instead, you must make a few dilutions of the highest standard DNA or RNA (Standard #2) in the Qubit™ kits to generate a standard curve with multiple data points.

Answer Id: E5016

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6d41993e0e849876c60460cbceeff265_FAQ

Can the Qubit™ kits give an indication of sample quality in nucleic acid samples?

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Answer

No. The Qubit™ DNA and RNA kits only quantify the amount of either DNA or RNA in the sample. The Qubit™ fluorometer cannot take absorbance readings to provide a A260/A280 ratio or detect protein in nucleic acid samples. This can be done with the NanoDrop™ instrument. If your sample contains protein or other contaminants that can affect the assay, it should be further purified.

If your sample may contain both DNA and RNA, one may use either (or both) the DNA and RNA Qubit™ kits and compare with samples treated with either RNase or DNase to get an accurate determination of DNA or RNA, respectively.

Answer Id: E5017

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d6fb20fed90cbf2dca39d90601dd2d06_FAQ

Why are my UV absorbance readings higher than the Qubit™ fluorometer readings?

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Answer

UV absorbance readings will measure DNA, RNA, proteins, free nucleotides, free bases and other aromatic molecules, etc., basically, everything and anything that absorbs at 260 nm. Since the Qubit™ assays use fluorescent dyes that generate a fluorescent signal upon binding to either DNA, RNA, or protein, the concentration data will be more accurate than that obtained traditionally using absorbance-based methods. For additional information on the Qubit™ fluorometer and associated reagents, search for "Qubit" on the Thermo Fisher Scientific website homepage.

Answer Id: E5018

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3b127a8d2c444385c85e942325ead35e_FAQ

Can the Qubit® Quantitation Platform do a good job of quantifying plasmids?

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Answer

Yes. If you are doing a typical plasmid miniprep with lots of DNA (over 50 ng/μL), you should use the Quant-iT™ DNA BR assay, as this will allow you to quantitate without making an extra dilution. If, on the other hand, you are doing “plasmid rescue” or some other method that gives you small amounts of DNA, then you should use the Quant-iT™ DNA HS assay.

Answer Id: E5019

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bd9a7d2336e310e4a99bbb5667e0cb74_FAQ

How does the accuracy and sensitivity of the Qubit™ quantitation assays using the Qubit™ fluorometer compare to a microplate reader?

Product FAQ

Answer

The accuracy and sensitivity of the Qubit™ quantitation assays are the same as that of a microplate reader. This was a requirement during product development. The detection limits for each Qubit™ kit can be found on the corresponding product manual, which can be found by searching our website by keyword or catalog number.

Answer Id: E5020

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