Can the Easy-Titer Antibody Assay kits be used with cell culture supernatant?

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Yes. Samples are diluted at least 20-fold (usually much more) in the assay dilution buffer, rendering them fully compatible with the bead agglutination method.

Answer Id: E8627

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Do the Easy-Titer Antibody Assay kits come with a microplate?

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No. Any standard, clear 96-well microplate can be used for this assay method.

Answer Id: E8628

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How do I pipette small volumes in the Easy-Titer Antibody Assay?

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Answer

The assay method requires many-fold dilution of sample (so that the target immunoglobulin is at 5-500 ng/mL). To obtain accurate results, this dilution must be done carefully. Use properly matched pipette sizes (e.g., do not use a 200 μl pipette to measure 10 μl) and make the dilution in two or three steps (e.g., 10-fold times 10-fold)

Answer Id: E8629

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Can Melon Gel purify chicken IgY?

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Answer

No. Thermo Scientific™ Thiophilic Adsorbent (Cat. Nos. 20500 and 44916) can be used to purify IgY from serum. The Chicken IgY Purification Kit (Cat. No. 44918) can be used to purify IgY from egg yolks.

Answer Id: E8647

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I am having problems with my standard curve in the Easy-Titer Antibody Assay. What do I need to do?

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Answer

Make sure that you are mixing the samples vigorously. We set our shaker near the highest setting for this step. For best results, the beads must be fully dispersed.

Answer Id: E8630

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I am using the BenchPro™ 2100 Plasmid Purification System. Can I elute the DNA in less TE?

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You can pipette out 700 μL of TE, so that the final DNA concentration could be higher. We would not recommend using less than 750 μL TE buffer.

Answer Id: E7760

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How stable are your cationic lipids that are used in transfection?

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Answer

All the following lipids are stable at 4º C for at least one year:
11668-019 Lipofectamine™ 2000
10362-100 Cellfectin™ II
10459-014 DMRIE-C
10964-013 Lipofectamine™ PLUS™
18292-011 Lipofectin™
18324-012 Lipofectamine™

Answer Id: E3211

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What are some of the common types of auxotrophic markers in yeast?

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The following are commonly employed auxotrophic markers:

1) his3Δ1: Histidine requiring strain (from gene disruption) with a deletion in locus 1. The his3 denotes the disruption of the HIS3 gene. The Δ1 is a deletion that has been engineered to decrease the recombination between the incoming plasmid DNA and the chromosomal site.
2) leu2: Leucine requiring strain due to the disruption of the LEU2 gene.
3) trp1-289: Tryptophan requiring strain, developed from gene disruption and a further point mutation to decrease the recombination between the incoming plasmid DNA and the chromosomal site.
4) ura3-52: Uracil requiring.

For more detail on types and methods of gene disruption in yeast refer to METHODS IN ENZYMOLOGY Vol. 194.

Answer Id: E3770

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How can I determine when the Melon Gel Support is saturated?

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Answer

For serum, a volume double the Melon Gel Support volume is the maximum amount that can be processed at one time. To determine if too much sample was processed, examine the purified IgG by SDS-PAGE for additional protein bands.

Answer Id: E8648

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What is the minimal magnification needed for the CISH™ signal to be seen on the bright field microscope?

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Answer

HER2 signals can be easily detected under 40x magnification.

Answer Id: E4999

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What other excitation wavelengths can I use to visualize SYBR™ Safe™ DNA Gel Stain?

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Answer

SYBR™ Safe™ DNA Gel Stain has two main excitation peaks: in the UV region at 280 nm, and in the visible region at 502 nm. Thus, 254 nm or 300 nm UV excitation will work, as will excitation with 488 nm lasers, 470 nm LEDs, and broad blue light sources (such as the Safe Imager™ Blue-Light Transilluminator (Cat. No. S37102). Maximal excitation occurs at 502 nm; the Safe Imager™ Blue-Light Transilluminatorr is therefore the best choice for excitation of SYBR™ Safe™ DNA Gel Stain. You can find the full excitation and emission spectra for SYBR™ Safe™ DNA Gel Stain online and also in the protocol provided with the stain.

Answer Id: E8057

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Will fetal bovine serum (FBS) in a sample interfere with the Easy-Titer Antibody assay?

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Answer

No, these assay use beads with target-specific antibodies, so off-target immunoglobulins have little or no effect on assay performance.

Answer Id: E8631

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How long is the ‘self test’ on the BenchPro™ 2100 instrument?

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Answer

A self test is performed each time a run is started and takes approximately 6 minutes to complete.

Answer Id: E7761

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Can the Countess™ II and Countess™ II FL instruments count bacterial cells?

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Answer

No, bacteria are too small to be distinguished from non-cell debris.

Answer Id: E12284

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How do I use linkage disequilibrium units (LDUs) to select SNP markers in the SNPbrowser™ Software?

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Answer

At least one SNP marker should be selected per LDU for good results; more conservatively, 2-3 per LDU should be chosen to account for population differences, lack of SNP informativeness for a study, assay and experimental difficulties, etc. For candidate regions with high LD (e.g., all SNPs clustered in a region of 0.05 LDU), only a subset of the available validated SNP markers may be needed for a study.

Use the SNP Wizard Tag SNP Selection tool to eliminate informatively redundant SNPs, potentially using allele frequency as a filtering criterion. For candidate regions with low LD, use the SNP Wizard SNP Density Selection tool to both detect any gaps in the LD map between validated SNPs, or between validated SNPs not meeting a desired allele frequency threshold, and to select additional non-validated SNPs to fill any gaps.

Answer Id: E2776

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