Why are my cells not adhering when I transfer them to a new flask?

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Answer

Trypsinize this type of cells for a shorter duration or use less trypsin or Gibco™ TrypLE™ Select or Gibco™ TrypLE™ Express Trypsin Replacement. After trypsinization dilute in 2 to 5 mL of cell culture growth medium transfer the cell suspension to 15 mL centrifuge tube. Centrifuge for 5-10 minutes at 100 x g. Discard the supernatant and suspend cell pellet in 2-5 mL fresh medium. Determine the count and seed the flask according to normal protocol and cell should adhere to the surface.

Answer Id: E11930

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What reagents do you offer for cell dissociation, and what are the main differences between them?

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Answer

Please use this selection chart that compares our cell dissociation reagents (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin.html).

Answer Id: E11900

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Do you have a non-animal-origin enzyme for cell dissociation?

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Answer

Yes, the TrypLE™ products contain rProtease, a non-animal, trypsin-like enzyme used for the dissociation of attachment dependent cell lines. TrypLE™ enzyme has demonstrated the ability to dissociate cells cultured both in serum-free and serum-supplemented systems. The catalog numbers for TrypLE™ Select are 12563-011 (100 mL) and 12563-029 (500 mL). Some example catalog numbers for TrypLE™ Express include 12604-013 without Phenol red (100 mL) and 12605-010 with Phenol red (100 mL). Both formulations are also available in additional sizes. All these products are animal origin-free.

Answer Id: E11903

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What is the difference between Gibco™ TrypLE™ Express and Gibco™ TrypLE™ Select?

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Answer

Both of the products contain rProtease, non-animal, trypsin-like enzyme formulation used for the dissociation of attachment-dependent cell lines. This alternative for porcine trypsin is a recombinant enzyme derived from microbial fermentation. The Express and Select products differ in their production and testing methods and in the documentation provided. TrypLE™ Select is for the industrial market and has release-testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF-dedicated equipment and has Intended Use for Research Use/Further Cell Culture Manufacturing. TrypLE™ Express is manufactured without use of dedicated equipment and is intended for Research Use Only. Not for use in diagnostic procedures. To read more about TrypLE™ and cell release times using this reagent, please click here (https://www.thermofisher.com/us/en/home/life-science/cell-culture/mammalian-cell-culture/reagents/trypsin/tryple-express.html#1).

Answer Id: E11902

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What can be used to detach cells from plates when coated with a thin layer of collagen I?

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Answer

Enzymes that can be used include: Gibco™ TrypLE™ Express Enzyme, Gibco™ TrypLE™ Select Enzyme, or Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent.

Answer Id: E11806

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What are the benefits of Gibco™ TrypLE™ reagent over trypsin?

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Answer

TrypLE™ reagent is free of animal- and human-derived components, producing an exceptionally pure reagent that is gentle on cells. Inactivation with trypsin inhibitors is not required. TrypLE™ reagent is ideal for dissociating a variety of attachment-dependent mammalian cell lines both in serum and in serum-free conditions, and can be directly substituted for trypsin in your current protocol. TrypLE™ reagent is room-temperature stable and ready to use when you need it. TrypLE™ cell dissociation reagents remain stable for 24 months at room temperature, making storage and handling easier and more convenient.

Answer Id: E11901

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Do you offer media without phenol red (phenol red-free media)?

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Answer

Yes, please visit this page for a list of our phenol red-free media (http://tools.thermofisher.com/content/sfs/brochures/Phenol_RedFree_Media_Glance.pdf).

Answer Id: E11881

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What is the difference between TrypLE™ Express and TrypLE™ Select?

Product FAQ

Answer

Both of the products contain rProtease, non-animal, trypsin-like enzyme formulations used for the dissociation of attachment dependent cell lines. This alternative for porcine Trypsin is a recombinant enzyme derived from microbial fermentation.

The Express and Select products differ in their production and testing methods and in the documentation provided. TrypLE™ Select is for the industrial market and has release testing both for endotoxins and enzyme activity. This material also has a Drug Master File on record. It is manufactured in AOF dedicated equipment and has Intended Use for Research Use/Further Cell Culture Manufacturing.

TrypLE™ Express is manufactured without use of dedicated equipment and is intended for Research Use Only, Not intended for human or animal diagnostic or therapeutic use.

Answer Id: E5567

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