These Product FAQs pertain to RUO products. These products are for Research Use Only. Not for use in diagnostic procedures. If using an IVD product, please refer to your specific product information.
I need to digest my plasmid with 2 or 3 different restriction enzymes. Can I perform a simultaneous double or even triple digestion?
If you are able to find a buffer in which all 3 enzymes have sufficient activity (usually not lower than 50%), you can set up a single digestion with all 3 enzymes. It is important that the total volume of enzymes you add to your reaction is not more than 1/10th of the total reaction volume. The reason for this is that some enzymes have star activity if the concentration of glycerol exceeds 5%. If you are not able to find a buffer in which all your enzymes have sufficient activity, you will have to perform sequential digestions of the plasmid with the individual enzymes.
Answer Id: E9848
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