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Brochure: Automated Sample Storage Resource Guide

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Specification Sheet: Matrix 2D Barcoded Storage Tubes

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Specification Sheet: Can 2D barcoded tubes resist DMSO and will they produce successful barcode reads following exposure to 100% DMSO solution?

Manual / Product Insert

PI: POP-7th Polymer - CG - For the 3730 Series Genetic Analyzer

Version: 4487170 Rev. A 18July2013
Catalog #
  • 4486277(Discontinued)

Product FAQ

How and when should I clean the "Water Trap" on the Applied Biosystems™ 3730 Series DNA Analyzer?

Answer

The "Water Trap" should be flushed weekly.

1) Fill a clean 20mL, all-plastic Luer lock syringe (Cat. No. 4324463) with clean distilled or deionized water. Expel any bubbles. NOTE: Do not use a syringe smaller than 20mL or excessive pressure may be generated.

2) Attach the syringe to the forward-facing Luer fitting. Hold the fitting with one hand while threading the syringe onto the fitting with the other hand.

3) Open the forward-facing Luer fitting by grasping the body of the fitting and turning it and the syringe approximately one-half turn counterclockwise.

4) Open the exit fitting at the left side of the pump block by turning it approximately one-half turn counterclockwise.

5) Hold an empty beaker under the left side fitting while pressing gently but steadily on the syringe plunger. IMPORTANT: Take approximately 30 seconds to flush 5mL of distilled or deionized water through the trap. Excessive pressure can damage the "Water Trap" seals. Make sure that the exit/waste seals are open, in order to avoid pressure buildup. Continue to gently flush the full 20ml volume through the trap.

6) Close the forward-facing Luer fitting first (hand-tight only).

7) Close the left side exit fitting second (hand-tight only).

8) Remove the syringe from the forward-facing Luer fitting by holding the fitting with one hand while removing the syringe counterclockwise with the other hand.

Answer Id: E2536

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Product FAQ

How many samples can I run with one 28 ml bottle of POP-7™ polymer on my Applied Biosystems™ 3730 Series DNA Analyzer?

Answer

8,000-12,000 samples

Answer Id: E2549

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Product FAQ

How long can I keep the buffer on the Applied Biosystems™ 3730/3730xl DNA Analyzer?

Answer

For the Applied Biosystems™ 3730/3730xl DNA Analyzer, the buffer can keep for 48 hours.

Answer Id: E12731

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Product FAQ

How do I turn on my Applied Biosystems™ 3730/3730xl DNA Analyzer?

Answer

The Applied Biosystems™ 3730/3730xl DNA Analyzer system includes both the instrument and the computer - you cannot start or restart one without starting the other. Improper boot sequence can lead to communication issues, aborted runs, and possible loss of data. The proper boot sequence is:

1. Turn on the computer and log in as the local administrator.
2. Turn on the power button on the instrument and wait for the green light.
3. Launch the Data Collection Software and wait until all 4 services turn green.

Step-by-step instructions for the start-up procedures can be found in the Applied Biosystems™ 3730/3730xl DNA Analyzers: Starting Up the Instrument Technical Note (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/3730-Start-Up-Procedures.pdf) and the Applied Biosystems™ 3730/3730xl DNA Analyzer Getting Started Guide (http://tools.thermofisher.com/content/sfs/manuals/4478016A.pdf).

Answer Id: E12723

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Product FAQ

Are bubbles in the "Water Trap" of the 3130 or 3730 systems acceptable?

Answer

A few small bubbles are acceptable; however, it is still a good idea to flush the "Water Trap" if there is any doubt. Excessive bubbles will decrease the efficiency of the "Water Trap" from keeping polymer out of the PDP mechanism. The "Water Trap" should be flushed weekly with a good, clean source of distilled/deionized water. Refer to FAQ "Where is the Water Trap and how do I clean it?"

Answer Id: E2537

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Product FAQ

How much polymer is consumed on the Applied Biosystems™ 3730 Series DNA Analyzer during a normal run?

Answer

The following volumes are approximations:

- 48-capillary 36cm array: 110µl
- 48-capillary 50cm array: 110µl
- 96-capillary 36cm array: 250µl
- 96-capillary 50cm array: 250µl

Regardless of the array type or length, sufficient over-filling is designed into the module to ensure the capillary is adequately purged prior to the next injection.

Answer Id: E2550

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Product FAQ

Do I need to replace the buffer every 2-3 days if the Applied Biosystems™ 3730/3730xl DNA Analyzer is not in use?

Answer

If the instrument is not in use, it is not necessary to replace the buffer every 2-3 days. However, the buffer level needs to remain at the fill line to prevent the capillary or array from drying out. Top off the buffer volume with water if necessary. Replace the anode and cathode buffer with freshly made 1X Running Buffer prior to starting a new run.

Answer Id: E12732

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