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How and when should I clean the "Water Trap" on the Applied Biosystems™ 3730 Series DNA Analyzer?

Product FAQ

Answer

The "Water Trap" should be flushed weekly.

1) Fill a clean 20mL, all-plastic Luer lock syringe (Cat. No. 4324463) with clean distilled or deionized water. Expel any bubbles. NOTE: Do not use a syringe smaller than 20mL or excessive pressure may be generated.

2) Attach the syringe to the forward-facing Luer fitting. Hold the fitting with one hand while threading the syringe onto the fitting with the other hand.

3) Open the forward-facing Luer fitting by grasping the body of the fitting and turning it and the syringe approximately one-half turn counterclockwise.

4) Open the exit fitting at the left side of the pump block by turning it approximately one-half turn counterclockwise.

5) Hold an empty beaker under the left side fitting while pressing gently but steadily on the syringe plunger. IMPORTANT: Take approximately 30 seconds to flush 5mL of distilled or deionized water through the trap. Excessive pressure can damage the "Water Trap" seals. Make sure that the exit/waste seals are open, in order to avoid pressure buildup. Continue to gently flush the full 20ml volume through the trap.

6) Close the forward-facing Luer fitting first (hand-tight only).

7) Close the left side exit fitting second (hand-tight only).

8) Remove the syringe from the forward-facing Luer fitting by holding the fitting with one hand while removing the syringe counterclockwise with the other hand.

Answer Id: E2536

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Are bubbles in the "Water Trap" of the 3130 or 3730 systems acceptable?

Product FAQ

Answer

A few small bubbles are acceptable; however, it is still a good idea to flush the "Water Trap" if there is any doubt. Excessive bubbles will decrease the efficiency of the "Water Trap" from keeping polymer out of the PDP mechanism. The "Water Trap" should be flushed weekly with a good, clean source of distilled/deionized water. Refer to FAQ "Where is the Water Trap and how do I clean it?"

Answer Id: E2537

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When using the Applied Biosystems™ 3730 Series DNA Analyzer, what does the "Validation Failed" message mean and how do I resolve it?

Product FAQ

Answer

This error appears when the there is a mismatch between the plate information and what is loaded or available on the system. The Event Viewer will usually specify what failed validation. If this message comes up, please check the following:

- Has a spatial calibration been run?

- A new spectral may be needed or be made active (or the wrong instrument protocol was selected for the run).

- The plate record may indicate one type of plate is being used but another was loaded (e.g. plate record may say that the plate is septa sealed but the plate is actually heat sealed).

Answer Id: E2568

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What is the difference between the Water Wash Wizard and the Water Trap Flush on the 3730 Series?

Product FAQ

Answer

The Water Wash Wizard is an automated procedure to clean the block, polymer channels, and tubing of the Applied Biosystems™ 3730 Series DNA Analyzer. The Water Trap Flush is a manual procedure to clean/refill water in the Water Trap of the Applied Biosystems™ 3730 Series DNA Analyzer's Polymer Delivery Pump (PDP) block. Also refer to FAQ "What is the "Water Trap"?

Answer Id: E2539

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How are the blocks, channels, and polymer tubing cleaned on the Applied Biosystems™ 3730 Series?

Product FAQ

Answer

The Polymer Delivery Pump (PDP) and lower block, polymer channels, and tubing can and should be cleaned with all hardware components on the instrument. Run the Water Wash Wizard to clean the pump block, lower block, polymer channels, and polymer tubing. This should be performed monthly or as needed. Flush the Water Trap on a weekly basis by loosening the valves to the trap, attaching the syringe to the valve facing you and pushing water through while capturing the flow through in a beaker held under the other valve.

Answer Id: E5584

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How long can Running Buffer stay on the Applied Biosystems™ 3730 Series DNA Analyzer?

Product FAQ

Answer

The Running Buffer on the Applied Biosystems™ 3730 Series DNA Analyzer has a use-life of 48 hours on the instrument. Replace the Running Buffer (in both the buffer jar and reservoirs) every 48 hours.

Answer Id: E2540

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