You searched for: 

4309849

Product FAQ

Can I restart my Applied Biosystems™ 3130/3130xl Genetic Analyzer daily?

Answer

There is no harm in restarting the system daily, but it should not be necessary unless there is an occasional loss in communication between the instrument and computer or the system is running significantly slower than normal. If the problems in run speed or communication loss are persistent, then it may be indicative of a larger problem. Technical Support should be contacted for further assistance at techsupport@thermofisher.com.

Answer Id: E12692

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

It is taking a longer time than normal to start the Applied Biosystems™ 3130/3130xl Genetic Analyzer Data Collection Software, what should I do?

Answer

If the system has not been started in the last week, completely shut off the instrument and the computer, wait for 2 minutes and restart it as directed in the Applied Biosystems™ 3130/3130xl Genetic Analyzers: Starting Up the Instrument Technical Note or the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4).

If the computer itself seems to be running slowly after restarting, you may need to defragment the hard drives. For the Applied Biosystems™ 3130/3130xl Genetic Analyzer, only defragment the C and E drives. Do not defragment the D and F drives - the Oracle database is installed on these drives, and defragmenting the Oracle drive may cause functionality issues with your Data Collection and GeneMapper™ software.

If the system has been restarted and the computer slowdown is limited to the instrument Data Collection Software, it is possible that the database/data store is getting full. For the Applied Biosystems™ 3130/3130xl Genetic Analyzer, after the Data Collection Software has launched, select “Database Manager” on the left side. If there are more than 300 runs or the database is greater than 10% full, you will need to click the Cleanup button to remove all Processed plates in order to increase the speed of the system. Should the database remain this way or continue to get filled, it will eventually hit a point where it will lock up and a service call will need to be opened for re-imaging and re-installation.

Answer Id: E12714

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

Which login should I use for the Applied Biosystems™ 3130/3130xl Genetic Analyzer computer?

Answer

The Data Collection and secondary analysis software requires certain permissions in order to function (Read, Write, Delete). To avoid issues with restrictions placed on user accounts, it is recommended that you log in either as Administrator or INSTR-ADMIN.

Answer Id: E12693

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

There is a large bubble at the tip of the piston on my Applied Biosystems™ 3130/3130xl Genetic Analyzer. How can I remove it?

Answer

It is not unusual to see a large bubble at the tip of the piston while performing the water wash wizard on the system. When the polymer is put back on to the system, the difference in viscosity is usually enough to dislodge the bubble and push it out. In extreme cases, it may be necessary to repeat the wash and go back and forth between the water and polymer to force it out.

Answer Id: E12715

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

How often should I perform maintenance on my Applied Biosystems™ 3130/3130xl Genetic Analyzer?

Answer

Maintenance tasks on the Applied Biosystems™ 3130 Series Genetic Analyzer are broken down into daily, weekly, monthly, and as-needed tasks. For detailed information on the maintenance steps and when to perform them, please refer to the Applied Biosystems™ 3130/3130xl Genetic Analyzers Maintenance Checklist(https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Maintenance%20Checklist.pdf) or the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).

Answer Id: E12694

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

How often should I change the array on the Applied Biosystems™ 3130/3130xl Genetic Analyzer?

Answer

The arrays are warrantied for a certain number of runs or one year from date of receipt, whichever comes first. If you notice that you are starting to get reduced data quality (e.g., shorter reads, trailing peaks in all 4 colors) even with the Sequencing Standard, these may be signs that the array requires changing.

Answer Id: E12700

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

With my Applied Biosystems™ 3130/3130xl Genetic Analyzer, I seem to be getting bubbles from the polymer bottle but the polymer level is normal. What is causing it?

Answer

It is possible that the polymer tube fitting may not be screwed into the pump properly, allowing air to get pulled in as the piston retracts. It is also possible that some debris may be stuck in the check valve, locking it in the open position. It may be necessary to detach and carefully attach the polymer tube again and then perform a water wash wizard to remove the bubbles and clear any debris from the check valve.

Answer Id: E12716

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

When I started my run on the Applied Biosystems™ 3130/3130xl Genetic Analyzer, an error occurred: “Leak detected” but I don't see any leaks. What else might cause this?

Answer

“Leak detected” error messages can also occur when the piston on the pump moves further than expected during the movement of the pump. The usual cause for this is a leak on the system, usually due to either an improperly tightened or improperly threaded fitting or a broken capillary. However, if this is not observed, some of the other reasons are:

1. Valve stuck in open position
2. Large air bubble under the piston
3. Polymer:
- Wrong polymer type (e.g., instrument thinks it has POP™-7 on it but it really has POP™-4)
- Old/degraded polymer
4. Improper/no maintenance on the system

Inspect the system for leaks. If you do not see any leaks on the system, perform a water wash using the water wash wizard and place fresh, non-expired polymer and freshly made 1X Running Buffer on the system. If the problem persists, a service call may be required.

Answer Id: E12711

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

I am only using the Applied Biosystems™ 3130/3130xl Genetic Analyzer once a week. Should I leave the polymer on the instrument or put it back in the refrigerator?

Answer

Putting the polymer back at 2 - 8 degrees C may extend the life of the polymer, however, we do not have any data indicating how long it might extend it. Removing the polymer and putting it back on the system can also result in a trade-off in usage. When placing it back on the system, you will need to degas the polymer for 30 - 60 mins, refill the pump/block, array, and remove bubbles, which will cause you to use more polymer for maintenance than you would for running samples and may decrease the number of runs you can get out of a single polymer bottle/pouch.

Answer Id: E12698

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

How long can I keep the polymer on the Applied Biosystems™ 3130/3130xl Genetic Analyzer?

Answer

For the Applied Biosystems™ 3130/3130xl Genetic Analyzer, the polymer can remain on the instrument for a week.

Answer Id: E12695

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

I have a red blinking light on the Applied Biosystems™ 3130/3130xl Genetic Analyzer. What does it mean and how can I fix it?

Answer

A blinking red light on the instrument may indicate that a hardware component has failed or a possible communication disruption has occurred between the instrument and computer. Completely shut down the instrument, wait for 2 minutes, and then start up in the order documented in the Applied Biosystems™ 3130/3130xl Genetic Analyzers: Starting Up the Instrument Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Start%20Up%20Procedures.pdf) or the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).
If the instrument goes from a flashing amber light to red light, one or more of the hardware components has failed the hardware diagnostic, and a service call should be opened up.

Answer Id: E12707

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

Can I use Applied Biosystems™ 3730 DNA Analyzer 10X Running Buffer on the Applied Biosystems™ 3130/3130xl Genetic Analyzer?

Answer

We do not recommend mixing and matching the various instrument reagents as each is manufactured using different processes, tested as an entire system, and warrantied to perform to a minimum specification. Substituting reagents can compromise the integrity of the system and cannot be supported by our service or support specialists. In addition, the 10X Running Buffer expires 1 year after date of receipt and once diluted to 1X, it will last 30 days at 2 - 8 degrees C or 7 days at ambient temperature. This could potentially result in having to dispose of a large volume of 10X Running Buffer due to expiration if you are using it on a lower-throughput system.

Answer Id: E12701

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

My array is stuck in the pump on my Applied Biosystems™ 3130/3130xl Genetic Analyzer, even after loosening the knob. What can I do?

Answer

If the array is stuck in the pump, even after loosening the knob, squirt around the edge of the array head with distilled water and try to remove it again. If the array will not move, a service call may be required. Do not use excessive force or tools to try and remove it.

Answer Id: E12717

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

After my run finished on the Applied Biosystems™ 3130/3130xl Genetic Analyzer, I don't see any data files. Where is my data?

Answer

The location of the files is determined by the Results Group. Double check the Location tab in the Results Group selected for the run to make sure it has not been changed and the files saved to a different location.

On the Applied Biosystems™ 3130/3130xl Genetic Analyzer, you can click on Run History in the Data Collection Software, select your plate and go to the Re-extraction heading under Run History to try and recover the data files.

Some other causes of missing data include:

- If the system is networked and something interrupts the network connection then it might interrupt the connection between the instrument and the computer. Depending on when it occurred, you might be able to recover it on Re-extraction. If you are saving the samples to a network drive and the location is unavailable, the data may still be in the Oracle Database on the Applied Biosystems™ 3130/3130xl Genetic Analyzer and can be re-extracted.
- If the septa is old/re-used. We officially recommend that the plate septa be used one time only. However, we are aware that some of our customers will rinse the septa with water and re-use them. In those instances, the septa can become sticky and gummy over time and eventually start sticking to the array. In the best case scenario, the array will lift the plate off of the autosampler just enough to break the connection with the plate sensor and abort the run. The Event Log might show that the run aborted due to the user aborting it, so the run will appear to have completed or stopped with no errors and no data. Worst case scenario is that the plate remains on array as the autosampler starts to move, bending the electrodes and breaking the array. Replace the plate septa regularly (each run is recommended, but after no more than 4 - 5 rinses; do not use bleach, detergents, or solvents to clean the septa, it will cause them to dry out faster and risks contaminating the system).

Answer Id: E12712

Was this answer helpful?

Yes
No
Thank you for your response

Product FAQ

I have an Applied Biosystems™ 3130/3130xl Genetic Analyzer. My consumables have expired, but how long past expiration can I really use them?

Answer

Thermo Fisher Scientific can only provide warranty on performance on non-expired reagents. Using reagents past their expiration date is a risk and may compromise your data integrity.

Answer Id: E12699

Was this answer helpful?

Yes
No
Thank you for your response