My array is stuck in the pump on my Applied Biosystems™ 3130/3130xl Genetic Analyzer, even after loosening the knob. What can I do?

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Answer

If the array is stuck in the pump, even after loosening the knob, squirt around the edge of the array head with distilled water and try to remove it again. If the array will not move, a service call may be required. Do not use excessive force or tools to try and remove it.

Answer Id: E12717

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What is the white residue around the array knob on my Applied Biosystems™ 3130/3130xl Genetic Analyzer?

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Answer

When placing the array on the instrument, the last step in the process is to loosen the array knob and the instrument forces any air bubbles around the array tip through the threading and the middle of the array knob. Some polymer gets pushed through as well and when it dries up, it appears as a white, flaky residue. Use a damp, lint-free cloth to remove it. If more residue appears after the cleanup, it is possible that the array knob is not tightened enough or the array ferrule may require replacement.

Answer Id: E12718

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When I started my run on the Applied Biosystems™ 3130/3130xl Genetic Analyzer, an error occurred: “Leak detected” but I don't see any leaks. What else might cause this?

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Answer

“Leak detected” error messages can also occur when the piston on the pump moves further than expected during the movement of the pump. The usual cause for this is a leak on the system, usually due to either an improperly tightened or improperly threaded fitting or a broken capillary. However, if this is not observed, some of the other reasons are:

1. Valve stuck in open position
2. Large air bubble under the piston
3. Polymer:
- Wrong polymer type (e.g., instrument thinks it has POP™-7 on it but it really has POP™-4)
- Old/degraded polymer
4. Improper/no maintenance on the system

Inspect the system for leaks. If you do not see any leaks on the system, perform a water wash using the water wash wizard and place fresh, non-expired polymer and freshly made 1X Running Buffer on the system. If the problem persists, a service call may be required.

Answer Id: E12711

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There is a large bubble at the tip of the piston on my Applied Biosystems™ 3130/3130xl Genetic Analyzer. How can I remove it?

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Answer

It is not unusual to see a large bubble at the tip of the piston while performing the water wash wizard on the system. When the polymer is put back on to the system, the difference in viscosity is usually enough to dislodge the bubble and push it out. In extreme cases, it may be necessary to repeat the wash and go back and forth between the water and polymer to force it out.

Answer Id: E12715

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I am only using the Applied Biosystems™ 3130/3130xl Genetic Analyzer once a week. Should I leave the polymer on the instrument or put it back in the refrigerator?

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Answer

Putting the polymer back at 2 - 8 degrees C may extend the life of the polymer, however, we do not have any data indicating how long it might extend it. Removing the polymer and putting it back on the system can also result in a trade-off in usage. When placing it back on the system, you will need to degas the polymer for 30 - 60 mins, refill the pump/block, array, and remove bubbles, which will cause you to use more polymer for maintenance than you would for running samples and may decrease the number of runs you can get out of a single polymer bottle/pouch.

Answer Id: E12698

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How do I register/renew my Applied Biosystems™ 3130/3130xl Genetic Analyzer Data Collection Software?

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Answer

The initial software registration is performed with your Field Service Engineer during the instrument installation. Once the initial registration is done, you will need to renew the license annually. There is no cost associated with renewal, just a computer with internet access and email access.

For detailed instructions on renewing the license on the Applied Biosystems™ 3130/3130xl Genetic Analyzer, please refer to the Applied Biosystems™ 3130 Series Genetic Analyzers and 3730 Series DNA Analyzers: Renewing the Software License (Data Collection Software v.4) Technical Note (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/3730-3130-License-Renewal.pdf).

Answer Id: E12706

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I performed a run on my Applied Biosystems™ 3130/3130xl Genetic Analyzer and cannot view my run history. When I go to the view, the array viewer and capillary viewer are black. What should I do?

Product FAQ

Answer

It is possible that the instrument and computer had a communication issue, disassociating the data from the plate record, resulting in no history and no data files. If this happens, shut down the instrument and the computer, wait 2 minutes and restart the system following the instructions in the Applied Biosystems™ 3130/3130xl Genetic Analyzers: Starting Up the Instrument Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Start%20Up%20Procedures.pdf) or the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).

Answer Id: E12719

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How often should I change the array on the Applied Biosystems™ 3130/3130xl Genetic Analyzer?

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Answer

The arrays are warrantied for a certain number of runs or one year from date of receipt, whichever comes first. If you notice that you are starting to get reduced data quality (e.g., shorter reads, trailing peaks in all 4 colors) even with the Sequencing Standard, these may be signs that the array requires changing.

Answer Id: E12700

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How do I turn on my Applied Biosystems™ 3130/3130xl Genetic Analyzer?

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Answer

The Applied Biosystems™ 3130/3130xl Genetic Analyzer system includes both the instrument and the computer - you cannot start or restart one without starting the other. Improper boot sequence can lead to communication issues, aborted runs, and possible loss of data. The proper boot sequence is:

1. Turn on the computer and log in as the local administrator.
2. Turn on the power button on the instrument and wait for the green light.
3. Launch the Data Collection Software and wait until all 4 services turn green.

Step-by-step instructions for the start-up procedures can be found in the Applied Biosystems™ 3130/3130xl Genetic Analyzers: Starting Up the Instrument Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Start%20Up%20Procedures.pdf) and the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).

Answer Id: E12688

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The amber light won't blink and remains at a solid amber light when I start the Applied Biosystems™ 3130/3130xl Genetic Analyzer. What's wrong?

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Answer

The amber light starts to blink once the instrument has logged onto the computer using the User account (e.g., 3130User). Anything that prevents it from connecting will cause the amber light to remain solid. Some of the more frequent reasons for this happening include:

-User password has expired or changed
-Firewall is on
-Cable is plugged into the wrong port
-The wrong network card was configured to connect to the local network
-Bad network card

Answer Id: E12708

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After my run finished on the Applied Biosystems™ 3130/3130xl Genetic Analyzer, I don't see any data files. Where is my data?

Product FAQ

Answer

The location of the files is determined by the Results Group. Double check the Location tab in the Results Group selected for the run to make sure it has not been changed and the files saved to a different location.

On the Applied Biosystems™ 3130/3130xl Genetic Analyzer, you can click on Run History in the Data Collection Software, select your plate and go to the Re-extraction heading under Run History to try and recover the data files.

Some other causes of missing data include:

- If the system is networked and something interrupts the network connection then it might interrupt the connection between the instrument and the computer. Depending on when it occurred, you might be able to recover it on Re-extraction. If you are saving the samples to a network drive and the location is unavailable, the data may still be in the Oracle Database on the Applied Biosystems™ 3130/3130xl Genetic Analyzer and can be re-extracted.
- If the septa is old/re-used. We officially recommend that the plate septa be used one time only. However, we are aware that some of our customers will rinse the septa with water and re-use them. In those instances, the septa can become sticky and gummy over time and eventually start sticking to the array. In the best case scenario, the array will lift the plate off of the autosampler just enough to break the connection with the plate sensor and abort the run. The Event Log might show that the run aborted due to the user aborting it, so the run will appear to have completed or stopped with no errors and no data. Worst case scenario is that the plate remains on array as the autosampler starts to move, bending the electrodes and breaking the array. Replace the plate septa regularly (each run is recommended, but after no more than 4 - 5 rinses; do not use bleach, detergents, or solvents to clean the septa, it will cause them to dry out faster and risks contaminating the system).

Answer Id: E12712

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Can I purchase a computer from you or someone else and install the Applied Biosystems™ 3130/3130xl Genetic Analyzer software myself?

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Answer

You can purchase a computer for installation of the secondary analysis software packages (e.g., Sequencing Analysis, GeneMapper™ software) as long as the computer meets the software computer requirements (for processor speed, the minimum speed of the processor should be at least 3 GHz). In these cases, you can install the software yourself.

For the instrument computer, the computer system has a much more stringent requirement and undergoes thorough testing to make sure it can handle the flow of data from the instrument to the computer. The images for the computer system are streamlined to make sure they have the proper drivers for the computer components, and the Operating System is optimized to make sure there is nothing running in the background that might slow the computer down, take up memory, or conflict with our software. Purchasing a computer commercially can introduce too many variables in terms of hardware and software that can negatively affect the data collection process so we cannot support connecting it to the instrument.

Answer Id: E12721

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How often should I perform maintenance on my Applied Biosystems™ 3130/3130xl Genetic Analyzer?

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Answer

Maintenance tasks on the Applied Biosystems™ 3130 Series Genetic Analyzer are broken down into daily, weekly, monthly, and as-needed tasks. For detailed information on the maintenance steps and when to perform them, please refer to the Applied Biosystems™ 3130/3130xl Genetic Analyzers Maintenance Checklist(https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Maintenance%20Checklist.pdf) or the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).

Answer Id: E12694

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What anti-virus software can I install on my Applied Biosystems™ 3130/3130xl Genetic Analyzer computer?

Product FAQ

Answer

All of our systems have been tested with Norton™ Anti-Virus Software.

Answer Id: E12702

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How do I shut off my Applied Biosystems™ 3130/3130xl Genetic Analyzer?

Product FAQ

Answer

After performing the short-term or long-term shutdown wizards:

1. Click “Stop All” in the Service Console and wait until all 4 services turn green. Close the program.
2. Go to the Start menu and select Shut Down. On Windows™ 7, click the Windows icon in the lower left corner of the screen. Click the arrow next to “Sleep” and select “Shut Down”.
3. Click the power button on the instrument.

Step-by-step instructions for the shutdown procedures can be found in the Applied Biosystems™ 3130/3130xl Genetic Analyzers: Shutting Down the Instrument Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Shut%20Down%20Procedures.pdf) and the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).

Answer Id: E12690

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