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Product FAQ

What is the difference between TaqMan™ Gene Expression Assays and the Custom TaqMan™ Gene Expression Assays?

Answer

TaqMan™ Gene Expression Assays are off-the-shelf, quantitative, human gene expression assays based on the TaqMan™ probe-based chemistry. The assays are formulated into a 20X mix, QC tested and functionally tested.

The Custom TaqMan™ Gene Expression Assays are designed based on the target sequences submitted by the customer, then synthesized, formulated, and delivered with analytical quality control. The target sequence information can be submitted by the customer through online Custom Assay Design Tool. The integrity of the sequence information must be verified before the design process. When you submit a sequence, you have an option to let us run the bioinformatic evaluation of the target sequence to verify the integrity. You can also run the bioinformatic evaluation by yourself. This integrity verification can be accomplished through BLAST analysis or other methods of verifying sequence integrity.

Answer Id: E1840

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Product FAQ

Can I set up and store the TaqMan™ Fast Universal PCR Master Mix at room temperature if the instrument is in use?

Answer

For optimal results, we recommend to run the reaction plate right after setting up the reaction. If a reaction plate cannot be run within 2 hours of completing the reaction setup, then freeze/refrigerate the reaction plate until it can be loaded and run on the fast PCR instrument platform.

Answer Id: E2355

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Product FAQ

How much cDNA is recommended per TaqMan™ or Custom TaqMan™ Gene Expression reaction?

Answer

For optimal performance of the TaqMan™ and Custom TaqMan™ Gene Expression Assays, use 1 to 100 ng of cDNA per 50 ul total reaction volume. For more information, please consult the TaqMan™ Gene Expression Assay protocol and Custom TaqMan™ Gene Expression Assay protocol.

Answer Id: E2012

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Product FAQ

Can I run a reaction made with the standard TaqMan™ Universal PCR Master Mix in fast thermal cycling mode?

Answer

No, we do not recommend this approach. Since the standard TaqMan™ Universal PCR Master Mix is not optimized for running with fast thermal cycling, assay results will be significantly compromised.

Answer Id: E2353

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Product FAQ

Is there a TaqMan™ PCR Core Reagents kit for the Fast Real-Time PCR System?

Answer

No. The only Fast reagents that we offer are the TaqMan™ Fast Universal PCR Master Mix (2X) for single plex use, and Fast Advanced Master Mix for greater sensitivity and multiplexing use.

Answer Id: E2348

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Product FAQ

Which TaqMan™ assay types can be used when using the with TaqMan™ Fast Universal PCR Master Mix (P/N 4352042)?

Answer

The following research assays are supported using the TaqMan™ Fast Universal and/or Advanced Fast PCR Master Mix: TaqMan™ Gene Expression Assays, Custom TaqMan™ Gene Expression Assays, Custom primers and probes designed from Primer Express™ software-designed quantitative assays.

Answer Id: E2322

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Product FAQ

What are the storage conditions and the shelf life of the TaqMan™ Fast Universal PCR Master Mix?

Answer

We recommend a storage temperature of 4C and the shelf life is 9 months from the manufacturing date.

Answer Id: E2350

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Product FAQ

What are the storage conditions for the TaqMan™ and Custom TaqMan™ Gene Expression and SNP Genotyping Assays?

Answer

After shipment, the assay mixes should be stored at -15 C to -20 C to maximize stability. Freeze/thaws should be kept to a minimum (a maximum of 10 freeze-thaw cycles is recommended). Keep all TaqMan™ and Custom TaqMan™ Gene Expression and SNP Genotyping Assays protected from direct exposure to light. Excessive exposure to light may affect the fluorescent probes. If desired, the Assay Mix may be diluted with TE buffer or DNase-free Water. It is recommended to make working stocks from the stock solutions provided in these kits.

Answer Id: E2008

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Product FAQ

When preparing the TaqMan™ or Custom TaqMan™ Gene Expression Assays, what is the recommended reaction volume?

Answer

When preparing the TaqMan™ or Custom TaqMan™ Gene Expression Assays, the recommended and supported volume is 50ul for a 96-well standard plate set-up; 20ul for a 96-well fast plate set-up and 10 ul for a 384-well set-up. For more information, please consult the TaqMan™ Gene Expression Assays Custom TaqMan™ Gene Expression Assays protocols.

Answer Id: E2014

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Product FAQ

What is the bookmarking feature in the TaqMan™ Genotyper™ Software, and how would I use it?

Answer

Bookmarking is a unique feature in TaqMan™ Genotyper™ Software that allows you to tag a data point or well while reviewing results in a Study. For example, in reviewing a cluster plot for an assay, a data point is observed to be somewhat between clusters. You can set a bookmark for this data point to denote this well for further investigation. The bookmark persists between the Results workspace and Quality Control workspace, so you can easily identify the data point in a cluster plot, experiment plate view, or on the samples tab. Bookmarks are cleared upon exit from a Study or exit from the application.

Answer Id: E7536

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Product FAQ

Can I use TaqMan™ Fast Universal PCR Master Mix for both one-step and two-step RT-PCR?

Answer

TaqMan™ Fast Universal PCR Master Mix is recommended for two-step RT-PCR, but for one-step reactions we recommend using TaqMan™ Fast Virus 1-Step Master Mix as the preferred reagent.

Answer Id: E2346

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Product FAQ

Is the TaqMan™ Fast Universal PCR Master Mix offered with AmpErase™ UNG?

Answer

The TaqMan™ Fast Universal PCR Master Mix is provided without any UNG and you should add in AmpErase™UNG to reactions separately, with the addition of an initial 2 minute cycling step at 50º C. If you prefer to have a master mix with the UNG pre-added, please use TaqMan™ Fast Advanced Master Mix.

Answer Id: E2352

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Product FAQ

What is the recommended input amount of template for use with the TaqMan™ Fast Universal PCR Master Mix?

Answer

We recommend adding 10 to 100 ng of RNA converted to cDNA as your template.

Answer Id: E2349

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Product FAQ

I am getting the message: “An error has occurred. See the log file C:ProgramFilesApplied BiosystemsTaqMan Genotyperconfigeclipse^3113099385.log.” How can I fix this?

Answer

1.Go to the Start button, then Programs, then TaqMan™ Genotyper Software
2.Right-click on the program and choose “Run as Administrator”
3.If that does not work, go back to the same menu and choose “Properties”
4.Choose the “Compatibility” tab, and check “Run this program as administrator”
5.Click “Apply”
6.You may+C69 have to restart the computer for the settings to apply

Answer Id: E7535

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Product FAQ

What component in the TaqMan™ Fast Universal PCR Master Mix or (P/N 4352042) allows rapid amplification results?

Answer

The hot-start DNA polymerase enzyme system in the TaqMan™ Fast Universal PCR Master Mix does not require an activation step, and can be activated significantly shorter hold times for the thermal cycling stages of PCR, thereby minimizing PCR run time. For more information on using this reagent, please consult the TaqMan™ Fast Universal PCR Master Mix (2X) protocol.

Answer Id: E2345

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