For my Applied Biosystems™ 3730/3730xl DNA Analyzer, the data service will not go to green. What should I do?

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Answer

1.Completely shut down the instrument and computer. Wait 2 minutes and restart the computer, log in, then turn on the instrument and wait for the green light.
2. Right click on “Computer” and select “Manage”. Expand “Services and Applications” and click on “Services”
3. Scroll down to “Oracle” in the list on the right. Any Oracle service that has a startup type of “Automatic” should have a Status of “Started”. If not, select the service and click Start.
After the services have started, wait 2 minutes and then try to launch the Data Collection Software.

If the services do not start or if this does not resolve the issue, please contact Technical Support at techsupport@thermofisher.com for further assistance. This symptom may require a service call.

Answer Id: E12748

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It is taking a longer time to start the Applied Biosystems™ 3730/3730xl DNA Analyzer Data Collection Software, what should I do?

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If the system has not been started in the last week, completely shut off the instrument and the computer, wait for 2 minutes and restart it in the order in the Applied Biosystems™ 3730/3730xl DNA Analyzers: Starting Up the Instrument Technical Note or the Applied Biosystems™ 3730/3730xl DNA Analyzer Getting Started Guide.

If the computer itself seems to be running slowly after restarting, you may need to defragment the hard drives. For the Applied Biosystems™ 3730/3730xl DNA Analyzer, only defragment the C and E drives. Do not defragment the D and F drives-the Oracle database is installed on these drives and defragmenting the Oracle drive may cause functionality issues with your Data Collection and GeneMapper™ software.

If the system has been restarted and the computer slowdown is limited to the instrument Data Collection Software, it's possible that the database/data store is getting full. For the Applied Biosystems™ 3730/3730xl DNA Analyzer, after the Data Collection software has launched select “Database Manager” on the left side. If there are more than 300 runs or the database is greater than 10% full, you will need to click the Cleanup button to remove all Processed plates in order to increase the speed of the system. Should the database remain this way or continue to get filled, it will eventually hit a point where it will lock up and a service call will need to be opened for re-imaging and re-installation.

Answer Id: E12749

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There is a large bubble at the tip of the piston on my Applied Biosystems™ 3730/3730xl DNA Analyzer. How can I remove it?

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Answer

It is not unusual to see a large bubble at the tip of the piston while performing the water wash wizard on the system. When the polymer is put back on to the system, the difference in viscosity is usually enough to dislodge the bubble and push it out. In extreme cases, it may be necessary to repeat the wash and go back and forth between the water and polymer to force it out.

Answer Id: E12750

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With the Applied Biosystems™ 3130/3130xl Genetic Analyzer, can I save my files automatically to a network drive?

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Answer

If you map the network drive so it shows up under Computer as a drive letter, then this feature can be configured from the Instrument Data Collection Software through the Results Group by going to the Destination tab, clicking “custom” and then browsing to the location on the network.

Answer Id: E12704

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With my Applied Biosystems™ 3730/3730xl DNA Analyzer, I seem to be getting bubbles from the polymer bottle but the polymer level is normal. What is causing it?

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Answer

It is possible that the polymer tube fitting may not be screwed into the pump properly, allowing air to get pulled in as the piston retracts. It is also possible that some debris may be stuck in the check valve, locking it in the open position. It may be necessary to detach and carefully attach the polymer tube again and then perform a water wash wizard to remove the bubbles and clear any debris from the check valve.

Answer Id: E12751

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I am only using the Applied Biosystems™3500/3500xL Genetic Analyzer once a week. Should I leave the polymer on the instrument or put it back in the refrigerator?

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Answer

Putting the polymer back at 2-8 degrees C may extend the life of the polymer, however, we do not have any data indicating how long it might extend it. Removing the polymer and putting it back on the system can also result in a trade-off in usage. When placing it back on the system, you will need to degas the polymer for 30-60 min, refill the pump/block, array, and remove bubbles, which will cause you to use more polymer for maintenance than you would for running samples and may decrease the number of runs you can get out of a single polymer bottle/pouch.

Answer Id: E12768

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How often should I perform maintenance on my Applied Biosystems™3500/3500xL Genetic Analyzer?

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Answer

Maintenance tasks on the Applied Biosystems™3500 series Genetic Analyzer are broken down into daily, weekly, monthly, and as-needed tasks. The Maintenance calendar in the Applied Biosystems™3500 series Data Collection Software can provide a monthly or daily view of tasks scheduled for the instrument. When a task is due to be performed, it will show up in the Dashboard view under Calendar Reminders. For detailed information on the maintenance steps and when to perform them, please refer to the Applied Biosystems™3500/3500xL Genetic Analyzers Maintenance Checklist (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3500%20Maintenance%20Checklist.pdf) or the Applied Biosystems™3500/3500xL Genetic Analyzer Getting Started Guide (Data Collection Software v.3.1) (https://tools.thermofisher.com/content/sfs/manuals/100031809_3500_3500xL_Software_v3_1_UG.pdf).

Answer Id: E12764

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I have an Applied Biosystems™ 3130/3130xl Genetic Analyzer. When do my consumables expire?

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Answer

If we do not specify the expiry date or number of uses, the warranty will last for 12 months from the date we deliver the consumable.

Answer Id: E12705

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My array is stuck in the pump on my Applied Biosystems™ 3730/3730xl DNA Analyzer, even after loosening the knob. What can I do?

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Answer

If the array is stuck in the pump, even after loosening the knob, squirt around the edge of the array head with distilled water and try to remove it again. If the array will not move, a service call may be required. Do not use excessive force or tools to try and remove it.

Answer Id: E12752

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My Applied Biosystems™3500/3500xL Genetic Analyzer consumables have expired, but how long past expiration can I really use them?

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Answer

Thermo Fisher Scientific can only provide warranty on performance on non-expired reagents. Using reagents past their expiration date is a risk and may compromise your data integrity.

Answer Id: E12769

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When I started my run on the Applied Biosystems™ 3730/3730xl DNA Analyzer, an error occurred: “Unstable Electrophoresis current detected, check for air bubbles” but I don't see any bubbles. What else can cause this?

Product FAQ

Answer

Some of the other causes of an Unstable Electrophoresis current detected error message are:

1.Leak on the system
2.Polymer that:
-Has expired
-Has been left on the instrument for more than the recommended time
-Is a mixture of expired polymer and non-expired polymer
3.Using Running Buffer that was:
-Diluted to 1X incorrectly
-Swapped with the water position on the
Autosampler deck
-On the instrument longer than 48 hours
-Not filled to the fill line or evaporated below the fill line
4. An arcing event that was not cleaned afterwards using the water wash wizard
5. Not performing regular maintenance on the instrument
6. Hardware issues

Inspect the system for leaks. If you do not see any leaks on the system, perform a water wash using the water wash wizard and place fresh, non-expired polymer and freshly made 1X Running Buffer on the system. If the problem persists, a service call may be required.

Answer Id: E12745

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How long can I keep the polymer on the Applied Biosystems™3500/3500xL Genetic Analyzer?

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Answer

For the Applied Biosystems™3500 Series Genetic Analyzer, the polymer can remain on the instrument for 14 days if room temperature is <25 degrees C and for 7 days if >25 degrees C.

Answer Id: E12765

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How often should I perform maintenance on my Applied Biosystems™ 3730/3730xl DNA Analyzer?

Product FAQ

Answer

Maintenance tasks on the Applied Biosystems™ 3730 series DNA Analyzer are broken down into daily, weekly, monthly, and as-needed tasks. For detailed information on the maintenance steps and when to perform them, please refer to the Applied Biosystems™ 3730/3730xl DNA Analyzers Maintenance Checklist Technical Note (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/3730-Maintenance-Checklist.pdf) or the Applied Biosystems™ 3730/3730xl DNA Analyzer Maintenance and Troubleshooting Guide (http://tools.thermofisher.com/content/sfs/manuals/3730SR_3730XLR_4359473_Maint_TroubShoot_UG.pdf).

Answer Id: E12729

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The amber light on my Applied Biosystems™3500/3500xL Genetic Analyzer continues to blink and won't proceed to green like it normally does. What's wrong?

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Answer

A perpetually blinking amber light usually indicates that there is a door open on the instrument or a bad door sensor. Open the main door, the oven door and the detector cell door (small black door with the laser warning on it) and close them again. After the autosampler stops moving the light should change to green. If the light does not go to green, it is possible that a door sensor is not working properly and a service call may be required.

Answer Id: E12777

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Do I need to replace the buffer every 2-3 days if the Applied Biosystems™ 3730/3730xl DNA Analyzer is not in use?

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Answer

If the instrument is not in use, it is not necessary to replace the buffer every 2-3 days. However, the buffer level needs to remain at the fill line to prevent the capillary or array from drying out. Top off the buffer volume with water if necessary. Replace the anode and cathode buffer with freshly made 1X Running Buffer prior to starting a new run.

Answer Id: E12732

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