Can I re-install the Applied Biosystems™ 3130/3130xl Genetic Analyzer Data Collection Software myself?

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Answer

Installation of the Data Collection Software for the Applied Biosystems™ 3130/3130xl Genetic Analyzer is a complex process that requires a Field Service Engineer to install the software properly.

Answer Id: E12720

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Do I need to replace the buffer every 2 - 3 days if the Applied Biosystems™ 3130/3130xl Genetic Analyzer is not in use?

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If the instrument is not in use, it is not necessary to replace the buffer every 2 - 3 days. However, the buffer level needs to remain at the fill line to prevent the capillary or array from drying out. Top off the buffer volume with water if necessary. Replace the anode and cathode buffer with freshly made 1X Running Buffer prior to starting a new run.

Answer Id: E12697

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With the Applied Biosystems™3500/3500xL Genetic Analyzer, can I save my files automatically to a network drive?

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If you map the network drive so it shows up under Computer as a drive letter, then this feature can be configured from the Instrument Data Collection Software through the Results Group by going to the Destination tab, clicking “custom” and then browsing to the location on the network. For Applied Biosystems™3500 series Genetic Analyzers, additional steps will be required. Please contact Technical Support for more information.

Answer Id: E12773

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Can I purchase a computer from you or someone else and install the Applied Biosystems™ 3130/3130xl Genetic Analyzer software myself?

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You can purchase a computer for installation of the secondary analysis software packages (e.g., Sequencing Analysis, GeneMapper™ software) as long as the computer meets the software computer requirements (for processor speed, the minimum speed of the processor should be at least 3 GHz). In these cases, you can install the software yourself.

For the instrument computer, the computer system has a much more stringent requirement and undergoes thorough testing to make sure it can handle the flow of data from the instrument to the computer. The images for the computer system are streamlined to make sure they have the proper drivers for the computer components, and the Operating System is optimized to make sure there is nothing running in the background that might slow the computer down, take up memory, or conflict with our software. Purchasing a computer commercially can introduce too many variables in terms of hardware and software that can negatively affect the data collection process so we cannot support connecting it to the instrument.

Answer Id: E12721

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I am only using the Applied Biosystems™ 3130/3130xl Genetic Analyzer once a week. Should I leave the polymer on the instrument or put it back in the refrigerator?

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Answer

Putting the polymer back at 2 - 8 degrees C may extend the life of the polymer, however, we do not have any data indicating how long it might extend it. Removing the polymer and putting it back on the system can also result in a trade-off in usage. When placing it back on the system, you will need to degas the polymer for 30 - 60 mins, refill the pump/block, array, and remove bubbles, which will cause you to use more polymer for maintenance than you would for running samples and may decrease the number of runs you can get out of a single polymer bottle/pouch.

Answer Id: E12698

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I have an Applied Biosystems™3500/3500xL Genetic Analyzer. When do my consumables expire?

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If we do not specify the expiry date or number of uses, the warranty will last for 12 months from the date we deliver the consumable.

Answer Id: E12774

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My array is stuck in the pump on my Applied Biosystems™ 3730/3730xl DNA Analyzer, even after loosening the knob. What can I do?

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Answer

If the array is stuck in the pump, even after loosening the knob, squirt around the edge of the array head with distilled water and try to remove it again. If the array will not move, a service call may be required. Do not use excessive force or tools to try and remove it.

Answer Id: E12752

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With my Applied Biosystems™ 3130/3130xl Genetic Analyzer, the start button is not active after linking my plate. Why?

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A patch is available here(http://resource.thermofisher.com/pages/WE28396/) to fix this.

Answer Id: E12722

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The amber light won't blink and remains at a solid amber light when I start the Applied Biosystems™ 3730/3730xl DNA Analyzer. What's wrong?

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Answer

The amber light starts to blink once the instrument has logged onto the computer using the User account (e.g. 3730User). Anything that prevents it from connecting will cause the amber light to remain solid. Some of the more frequent reasons for this happening include:

-User password has expired or changed
-Firewall is on
-Cable is plugged into the wrong port
-The wrong network card was configured to connect to the local network
-Bad network card

Answer Id: E12743

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When should I perform a short-term shutdown of my Applied Biosystems™3500/3500xL Genetic Analyzer versus a long-term shutdown?

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Answer

For the Applied Biosystems™3500 Series Genetic Analyzer, if the instrument will be idle for 1- 2 weeks, perform the short-term shutdown of the system. If the instrument will be idle for more than 2 weeks, perform the long-term shutdown using the Instrument Shutdown Wizard.

Answer Id: E12759

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I have an Applied Biosystems™ 3130/3130xl Genetic Analyzer. My consumables have expired, but how long past expiration can I really use them?

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Answer

Thermo Fisher Scientific can only provide warranty on performance on non-expired reagents. Using reagents past their expiration date is a risk and may compromise your data integrity.

Answer Id: E12699

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Can I purchase a computer from you or someone else and install the Applied Biosystems™3500/3500xL Genetic Analyzer software myself?

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Answer

You can purchase a computer for installation of the secondary analysis software packages (e.g., Sequencing Analysis, GeneMapper™software) as long as the computer meets the software compute requirements (for processor speed, the minimum speed of the processor should be at least 3 GHz) and you can install the software yourself.

For the instrument computer, the computer system has a much more stringent requirement and undergoes thorough testing to make sure it can handle the flow of data from the instrument to the computer. The images for the computer system are streamlined to make sure they have the proper drivers for the computer components, and the Operating System is optimized to make sure there is nothing running in the background that might slow the computer down, take up memory, or conflict with our software. Purchasing a computer commercially can introduce too many variables in terms of hardware and software that can negatively affect the data collection process so we cannot support connecting it to the instrument.

Answer Id: E12787

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How do I register/renew my Applied Biosystems™3500/3500xL Genetic Analyzer Data Collection Software?

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Answer

The initial software registration is performed with your Field Service Engineer during the instrument installation. Once the initial registration is done, you will need to renew the license annually. There is no cost associated with renewal, just a computer with internet access and email access.

For detailed instructions on renewing the license on the Applied Biosystems™3500/3500xL Genetic Analyzers: Renewing the Software License (Data Collection v.2.0, 3.0, 3.1) Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3500%20License%20Renewal.pdf).

Answer Id: E12775

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How often should I perform maintenance on my Applied Biosystems™3500/3500xL Genetic Analyzer?

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Answer

Maintenance tasks on the Applied Biosystems™3500 series Genetic Analyzer are broken down into daily, weekly, monthly, and as-needed tasks. The Maintenance calendar in the Applied Biosystems™3500 series Data Collection Software can provide a monthly or daily view of tasks scheduled for the instrument. When a task is due to be performed, it will show up in the Dashboard view under Calendar Reminders. For detailed information on the maintenance steps and when to perform them, please refer to the Applied Biosystems™3500/3500xL Genetic Analyzers Maintenance Checklist (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3500%20Maintenance%20Checklist.pdf) or the Applied Biosystems™3500/3500xL Genetic Analyzer Getting Started Guide (Data Collection Software v.3.1) (https://tools.thermofisher.com/content/sfs/manuals/100031809_3500_3500xL_Software_v3_1_UG.pdf).

Answer Id: E12764

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I have an Applied Biosystems™ 3730/3730xl DNA Analyzer. When do my consumables expire?

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Answer

If we do not specify the expiry date or number of uses, the warranty will last for 12 months from the date we deliver the consumable.

Answer Id: E12740

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