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Product FAQ

How do I turn on my Applied Biosystems™ 3730/3730xl DNA Analyzer?

Answer

The Applied Biosystems™ 3730/3730xl DNA Analyzer system includes both the instrument and the computer - you cannot start or restart one without starting the other. Improper boot sequence can lead to communication issues, aborted runs, and possible loss of data. The proper boot sequence is:

1. Turn on the computer and log in as the local administrator.
2. Turn on the power button on the instrument and wait for the green light.
3. Launch the Data Collection Software and wait until all 4 services turn green.

Step-by-step instructions for the start-up procedures can be found in the Applied Biosystems™ 3730/3730xl DNA Analyzers: Starting Up the Instrument Technical Note (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/3730-Start-Up-Procedures.pdf) and the Applied Biosystems™ 3730/3730xl DNA Analyzer Getting Started Guide (http://tools.thermofisher.com/content/sfs/manuals/4478016A.pdf).

Answer Id: E12723

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Product FAQ

When should I perform a short-term shutdown of my Applied Biosystems™ 3730/3730xl DNA Analyzer versus a long-term shutdown?

Answer

For the Applied Biosystems™ 3730/3730xl DNA Analyzer, if the instrument will be idle for less than a week, perform the short-term shutdown of the system. If it will be idle for longer than a week, follow the instructions in the user guide for a long-term shutdown using the Instrument Shutdown Wizard.

Answer Id: E12724

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Product FAQ

How do I shut off my Applied Biosystems™ 3730/3730xl DNA Analyzer?

Answer

After performing the short-term or long-term shutdown wizards:

1. Click “Stop All” in the Service Console and wait until all 4 services turn green. Close the program.
2. Go to the Start menu and select Shut Down. On Windows™ 7, click the Windows icon in the lower left corner of the screen. Click the arrow next to “Sleep” and select “Shut Down”.
3. Click the power button on the instrument.

Step-by-step instructions for the shutdown procedures can be found in the Applied Biosystems™ 3730/3730xl DNA Analyzers: Shutting Down the Instrument Technical Note (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/3730-Shut-Down-Procedures.pdf) and the Applied Biosystems™ 3730/3730xl DNA Analyzer Maintenance and Troubleshooting Guide (http://tools.thermofisher.com/content/sfs/manuals/3730SR_3730XLR_4359473_Maint_TroubShoot_UG.pdf).

Answer Id: E12725

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Product FAQ

How often should I restart my Applied Biosystems™ 3730/3730xl DNA Analyzer?

Answer

We recommend that you perform a restart of the system once a week. The system may require a restart more frequently if you experience issues related to a communication interruption between the instrument and computer. Some systematic issues include:

1. The software freezing or becoming unresponsive
2. The run timer not counting down or flashing back and forth between 2 times
3. No data present at the end of the run

In the event of a communication issue between the instrument and computer, close the Data Collection Software (perform a forced shutdown of the application through the Task Manager if Data Collection is unresponsive), completely power down the instrument and turn off the computer. To properly clear the memory, power down the system for at least 2 minutes prior to a restart.

Answer Id: E12726

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Product FAQ

The amber light won't blink and remains at a solid amber light when I start the Applied Biosystems™ 3730/3730xl DNA Analyzer. What's wrong?

Answer

The amber light starts to blink once the instrument has logged onto the computer using the User account (e.g. 3730User). Anything that prevents it from connecting will cause the amber light to remain solid. Some of the more frequent reasons for this happening include:

-User password has expired or changed
-Firewall is on
-Cable is plugged into the wrong port
-The wrong network card was configured to connect to the local network
-Bad network card

Answer Id: E12743

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Product FAQ

Can I restart my Applied Biosystems™ 3730/3730xl DNA Analyzer daily?

Answer

There is no harm in restarting the system daily, but it should not be necessary unless there is an occasional loss in communication between the instrument and computer or the system is running significantly slower than normal. If the problems in run speed or communication loss are persistent, then it may be indicative of a larger problem. Technical Support should be contacted for further assistance at techsupport@thermofisher.com.

Answer Id: E12727

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Product FAQ

When I started my run on the Applied Biosystems™3500/3500xL Genetic Analyzer, an error occurred: “Leak detected” but I don't see any leaks. What else might cause this?

Answer

“Leak detected” error messages can also occur when the piston on the pump moves further than expected during the movement of the pump. The usual cause for this is a leak on the system, usually due to an improperly tightened or threaded fitting or a broken capillary. However, if this is not observed, some of the other reasons include:

1. Valve stuck in open position
2. Large air bubble under the piston
3. Polymer:
-Wrong polymer type (e.g., instrument thinks it has POP™-7 on it but it really has POP™-4)
-Old/degraded polymer
4. Improper/no maintenance on the system

Inspect the system for leaks. If you do not see any leaks on the system, perform the wash the pump chamber and channels wizard using the conditioning pouch and place fresh, non-expired polymer and fresh Anode and Cathode buffer containers. If the problem persists, a service call may be required.

Answer Id: E12780

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Product FAQ

The amber light on my Applied Biosystems™ 3730/3730xl DNA Analyzer continues to blink and won't proceed to green like it normally does. What is wrong?

Answer

A perpetually blinking amber light usually indicates that there is a door open on the instrument or a bad door sensor. Open the main door, the oven door and the detector cell door (small black door with the laser warning on it) and close them again. After the autosampler stops moving the light should change to green. If the amber light does not go to green, it is possible that a door sensor is not working properly and a service call may be required.

Answer Id: E12744

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Product FAQ

I am only using the Applied Biosystems™ 3730/3730xl DNA Analyzer once a week. Should I leave the polymer on the instrument or put it back in the refrigerator?

Answer

Putting the polymer back at 2 - 8 degree C may extend the life of the polymer, however, we do not have any data indicating how long it might extend it. Removing the polymer and putting it back on the system can also result in a trade-off in usage. When placing it back on the system, you will need to degas the polymer for 30 - 60 min, refill the pump/block, array, and remove bubbles, which will cause you to use more polymer for maintenance than you would for running samples and may decrease the number of runs you can get out of a single polymer bottle/pouch

Answer Id: E12733

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Product FAQ

I performed a run on my Applied Biosystems™ 3130/3130xl Genetic Analyzer and cannot view my run history. When I go to the view, the array viewer and capillary viewer are black. What should I do?

Answer

It is possible that the instrument and computer had a communication issue, disassociating the data from the plate record, resulting in no history and no data files. If this happens, shut down the instrument and the computer, wait 2 minutes and restart the system following the instructions in the Applied Biosystems™ 3130/3130xl Genetic Analyzers: Starting Up the Instrument Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Start%20Up%20Procedures.pdf) or the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).

Answer Id: E12719

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Product FAQ

There is a large bubble at the tip of the piston on my Applied Biosystems™ 3130/3130xl Genetic Analyzer. How can I remove it?

Answer

It is not unusual to see a large bubble at the tip of the piston while performing the water wash wizard on the system. When the polymer is put back on to the system, the difference in viscosity is usually enough to dislodge the bubble and push it out. In extreme cases, it may be necessary to repeat the wash and go back and forth between the water and polymer to force it out.

Answer Id: E12715

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Product FAQ

I have an Applied Biosystems™3500/3500xL Genetic Analyzer. When do my consumables expire?

Answer

If we do not specify the expiry date or number of uses, the warranty will last for 12 months from the date we deliver the consumable.

Answer Id: E12774

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Product FAQ

Do I need to replace the buffer every 14 days if the Applied Biosystems™3500/3500xL Genetic Analyzer is not in use?

Answer

If the instrument is not in use, it is not necessary to replace the buffer every 14 days. However, the buffer level needs to remain at the fill line to prevent the capillary or array from drying out. Top off the buffer volume with water if necessary. Replace the anode and cathode buffer with freshly made 1X Running Buffer prior to starting a new run.

Answer Id: E12767

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Product FAQ

When should I perform a short-term shutdown of my Applied Biosystems™3500/3500xL Genetic Analyzer versus a long-term shutdown?

Answer

For the Applied Biosystems™3500 Series Genetic Analyzer, if the instrument will be idle for 1- 2 weeks, perform the short-term shutdown of the system. If the instrument will be idle for more than 2 weeks, perform the long-term shutdown using the Instrument Shutdown Wizard.

Answer Id: E12759

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Product FAQ

Which login should I use for the Applied Biosystems™ 3730/3730xl DNA Analyzer computer?

Answer

The Data Collection and secondary analysis software requires certain permissions in order to function (Read, Write, Delete). To avoid issues with restrictions placed on user accounts, it is recommended that you log in either as Administrator or INSTR-ADMIN.

Answer Id: E12728

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