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Product FAQ

I have an Applied Biosystems™3500/3500xL Genetic Analyzer. When do my consumables expire?

Answer

If we do not specify the expiry date or number of uses, the warranty will last for 12 months from the date we deliver the consumable.

Answer Id: E12774

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Product FAQ

How often should I perform maintenance on my Applied Biosystems™ 3130/3130xl Genetic Analyzer?

Answer

Maintenance tasks on the Applied Biosystems™ 3130 Series Genetic Analyzer are broken down into daily, weekly, monthly, and as-needed tasks. For detailed information on the maintenance steps and when to perform them, please refer to the Applied Biosystems™ 3130/3130xl Genetic Analyzers Maintenance Checklist(https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Maintenance%20Checklist.pdf) or the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).

Answer Id: E12694

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Product FAQ

There is a large bubble at the tip of the piston on my Applied Biosystems™ 3730/3730xl DNA Analyzer. How can I remove it?

Answer

It is not unusual to see a large bubble at the tip of the piston while performing the water wash wizard on the system. When the polymer is put back on to the system, the difference in viscosity is usually enough to dislodge the bubble and push it out. In extreme cases, it may be necessary to repeat the wash and go back and forth between the water and polymer to force it out.

Answer Id: E12750

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Product FAQ

How do I register/renew my Applied Biosystems™3500/3500xL Genetic Analyzer Data Collection Software?

Answer

The initial software registration is performed with your Field Service Engineer during the instrument installation. Once the initial registration is done, you will need to renew the license annually. There is no cost associated with renewal, just a computer with internet access and email access.

For detailed instructions on renewing the license on the Applied Biosystems™3500/3500xL Genetic Analyzers: Renewing the Software License (Data Collection v.2.0, 3.0, 3.1) Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3500%20License%20Renewal.pdf).

Answer Id: E12775

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Product FAQ

How long can I keep the polymer on the Applied Biosystems™ 3130/3130xl Genetic Analyzer?

Answer

For the Applied Biosystems™ 3130/3130xl Genetic Analyzer, the polymer can remain on the instrument for a week.

Answer Id: E12695

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Product FAQ

How often should I perform maintenance on my Applied Biosystems™ 3730/3730xl DNA Analyzer?

Answer

Maintenance tasks on the Applied Biosystems™ 3730 series DNA Analyzer are broken down into daily, weekly, monthly, and as-needed tasks. For detailed information on the maintenance steps and when to perform them, please refer to the Applied Biosystems™ 3730/3730xl DNA Analyzers Maintenance Checklist Technical Note (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/3730-Maintenance-Checklist.pdf) or the Applied Biosystems™ 3730/3730xl DNA Analyzer Maintenance and Troubleshooting Guide (http://tools.thermofisher.com/content/sfs/manuals/3730SR_3730XLR_4359473_Maint_TroubShoot_UG.pdf).

Answer Id: E12729

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Product FAQ

I have an Applied Biosystems™ 3130/3130xl Genetic Analyzer. My consumables have expired, but how long past expiration can I really use them?

Answer

Thermo Fisher Scientific can only provide warranty on performance on non-expired reagents. Using reagents past their expiration date is a risk and may compromise your data integrity.

Answer Id: E12699

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Product FAQ

When should I run a spectral calibration on the Applied Biosystems™ 3730 Series DNA Analyzer?

Answer

A new spectral calibration should be run:

- Whenever a new dye set is used on an instrument.
- After any optics adjustment performed by a service engineer.
- If the instrument has been moved.
- If you see a decrease in spectral separation (pull-up and/or pull-down peaks).
- If you alter any conditions (dye set, array type, or array length).
- For G5-RCT, Any4dye-HDR, and S dye set users only: Any time the array detection cell is moved/repositioned.

Answer Id: E2531

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Product FAQ

How do I register/renew my Applied Biosystems™ 3130/3130xl Genetic Analyzer Data Collection Software?

Answer

The initial software registration is performed with your Field Service Engineer during the instrument installation. Once the initial registration is done, you will need to renew the license annually. There is no cost associated with renewal, just a computer with internet access and email access.

For detailed instructions on renewing the license on the Applied Biosystems™ 3130/3130xl Genetic Analyzer, please refer to the Applied Biosystems™ 3130 Series Genetic Analyzers and 3730 Series DNA Analyzers: Renewing the Software License (Data Collection Software v.4) Technical Note (https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/3730-3130-License-Renewal.pdf).

Answer Id: E12706

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Product FAQ

With my Applied Biosystems™ 3730/3730xl DNA Analyzer, I seem to be getting bubbles from the polymer bottle but the polymer level is normal. What is causing it?

Answer

It is possible that the polymer tube fitting may not be screwed into the pump properly, allowing air to get pulled in as the piston retracts. It is also possible that some debris may be stuck in the check valve, locking it in the open position. It may be necessary to detach and carefully attach the polymer tube again and then perform a water wash wizard to remove the bubbles and clear any debris from the check valve.

Answer Id: E12751

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Product FAQ

I performed a run on my Applied Biosystems™ 3130/3130xl Genetic Analyzer and cannot view my run history. When I go to the view, the array viewer and capillary viewer are black. What should I do?

Answer

It is possible that the instrument and computer had a communication issue, disassociating the data from the plate record, resulting in no history and no data files. If this happens, shut down the instrument and the computer, wait 2 minutes and restart the system following the instructions in the Applied Biosystems™ 3130/3130xl Genetic Analyzers: Starting Up the Instrument Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Start%20Up%20Procedures.pdf) or the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).

Answer Id: E12719

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Product FAQ

How often should I restart my Applied Biosystems™3500/3500xL Genetic Analyzer?

Answer

We recommend that you perform a restart of the system once a week. The system may require a restart more frequently if you experience issues related to a communication interruption between the instrument and computer. Some systematic issues include:

-The software freezing or becoming unresponsive
-The run timer not counting down or flashing back and forth between two different times
-No data present at the end of the run

In the event of a communication issue between the instrument and computer, close the Data Collection Software (perform a forced shutdown of the application through the Task Manager if Data Collection is unresponsive), completely power down the instrument and turn off the computer. To properly clear the memory, power down the system for at least 2 minutes prior to a restart

Answer Id: E12761

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Product FAQ

I have a red blinking light on the Applied Biosystems™3500/3500xL Genetic Analyzer. What does it mean and how can I fix it?

Answer

A blinking red light on the instrument may indicate that a hardware component has failed or a possible communication disruption has occurred between the instrument and computer. Completely shut down the instrument, wait for 2 minutes, and then start up as documented in the Applied Biosystems™3500/3500xL Genetic Analyzers: Starting Up the Instrument(https://www.thermofisher.com/content/dam/LifeTech/Documents/PDFs/3500-Start-Up-Procedures.pdf) or the Applied Biosystems™3500/3500xL Genetic Analyzer Getting Started Guide (Data Collection Software v.3.1)(https://tools.thermofisher.com/content/sfs/manuals/100031809_3500_3500xL_Software_v3_1_UG.pdf) If the instrument goes from a flashing amber light to red light, one or more of the hardware components has failed the hardware diagnostic, and a service call should be opened up.

Answer Id: E12776

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Product FAQ

How long can I keep the buffer on the Applied Biosystems™ 3130/3130xl Genetic Analyzer?

Answer

For the Applied Biosystems™ 3130/3130xl Genetic Analyzer, the buffer can keep for 48 hours.

Answer Id: E12696

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Product FAQ

How long can I keep the polymer on the Applied Biosystems™ 3730/3730xl DNA Analyzer?

Answer

For the Applied Biosystems™ 3730/3730xl DNA Analyzer, the polymer can remain on the instrument for a week.

Answer Id: E12730

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