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Product FAQ

Can I restart my Applied Biosystems™ 3130/3130xl Genetic Analyzer daily?

Answer

There is no harm in restarting the system daily, but it should not be necessary unless there is an occasional loss in communication between the instrument and computer or the system is running significantly slower than normal. If the problems in run speed or communication loss are persistent, then it may be indicative of a larger problem. Technical Support should be contacted for further assistance at techsupport@thermofisher.com.

Answer Id: E12692

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Product FAQ

I have an Applied Biosystems™ 3130/3130xl Genetic Analyzer. My consumables have expired, but how long past expiration can I really use them?

Answer

Thermo Fisher Scientific can only provide warranty on performance on non-expired reagents. Using reagents past their expiration date is a risk and may compromise your data integrity.

Answer Id: E12699

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Product FAQ

Which login should I use for the Applied Biosystems™ 3130/3130xl Genetic Analyzer computer?

Answer

The Data Collection and secondary analysis software requires certain permissions in order to function (Read, Write, Delete). To avoid issues with restrictions placed on user accounts, it is recommended that you log in either as Administrator or INSTR-ADMIN.

Answer Id: E12693

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Product FAQ

How often should I perform maintenance on my Applied Biosystems™ 3130/3130xl Genetic Analyzer?

Answer

Maintenance tasks on the Applied Biosystems™ 3130 Series Genetic Analyzer are broken down into daily, weekly, monthly, and as-needed tasks. For detailed information on the maintenance steps and when to perform them, please refer to the Applied Biosystems™ 3130/3130xl Genetic Analyzers Maintenance Checklist(https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Maintenance%20Checklist.pdf) or the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).

Answer Id: E12694

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Product FAQ

Are the MicroAmp™ Optical 96-Well Reaction Plates and MicroAmp™ Optical 384 -Well Reaction Plates autoclavable?

Answer

Our microplates are certified to be DNA, RNase and PCR inhibitor free as long as they are kept in the original packaging bag. They are not meant to be autoclaved.

Answer Id: E2500

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Product FAQ

Can I place an Optical Fast 96-Well plate onto a standard Applied Biosystems™ 7500 or 7300 Real-Time PCR System?

Answer

No. The fast plate will not fit correctly into the standard block and assay performance will be significantly compromised.

Answer Id: E2357

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Product FAQ

Can I purchase a computer from you or someone else and install the Applied Biosystems™ 3130/3130xl Genetic Analyzer software myself?

Answer

You can purchase a computer for installation of the secondary analysis software packages (e.g., Sequencing Analysis, GeneMapper™ software) as long as the computer meets the software computer requirements (for processor speed, the minimum speed of the processor should be at least 3 GHz). In these cases, you can install the software yourself.

For the instrument computer, the computer system has a much more stringent requirement and undergoes thorough testing to make sure it can handle the flow of data from the instrument to the computer. The images for the computer system are streamlined to make sure they have the proper drivers for the computer components, and the Operating System is optimized to make sure there is nothing running in the background that might slow the computer down, take up memory, or conflict with our software. Purchasing a computer commercially can introduce too many variables in terms of hardware and software that can negatively affect the data collection process so we cannot support connecting it to the instrument.

Answer Id: E12721

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Product FAQ

I performed a run on my Applied Biosystems™ 3130/3130xl Genetic Analyzer and cannot view my run history. When I go to the view, the array viewer and capillary viewer are black. What should I do?

Answer

It is possible that the instrument and computer had a communication issue, disassociating the data from the plate record, resulting in no history and no data files. If this happens, shut down the instrument and the computer, wait 2 minutes and restart the system following the instructions in the Applied Biosystems™ 3130/3130xl Genetic Analyzers: Starting Up the Instrument Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Start%20Up%20Procedures.pdf) or the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).

Answer Id: E12719

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Product FAQ

How often should I change the array on the Applied Biosystems™ 3130/3130xl Genetic Analyzer?

Answer

The arrays are warrantied for a certain number of runs or one year from date of receipt, whichever comes first. If you notice that you are starting to get reduced data quality (e.g., shorter reads, trailing peaks in all 4 colors) even with the Sequencing Standard, these may be signs that the array requires changing.

Answer Id: E12700

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Product FAQ

How long can I keep the polymer on the Applied Biosystems™ 3130/3130xl Genetic Analyzer?

Answer

For the Applied Biosystems™ 3130/3130xl Genetic Analyzer, the polymer can remain on the instrument for a week.

Answer Id: E12695

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Product FAQ

I have a red blinking light on the Applied Biosystems™ 3130/3130xl Genetic Analyzer. What does it mean and how can I fix it?

Answer

A blinking red light on the instrument may indicate that a hardware component has failed or a possible communication disruption has occurred between the instrument and computer. Completely shut down the instrument, wait for 2 minutes, and then start up in the order documented in the Applied Biosystems™ 3130/3130xl Genetic Analyzers: Starting Up the Instrument Technical Note (https://www.thermofisher.com//content/dam/LifeTech/Documents/PDFs/3130%20Start%20Up%20Procedures.pdf) or the Applied Biosystems™ 3130/3130xl Genetic Analyzers Getting Started Guide (Data Collection Software v.4)(https://tools.thermofisher.com/content/sfs/manuals/4477796.PDF).
If the instrument goes from a flashing amber light to red light, one or more of the hardware components has failed the hardware diagnostic, and a service call should be opened up.

Answer Id: E12707

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Product FAQ

What anti-virus software can I install on my Applied Biosystems™ 3130/3130xl Genetic Analyzer computer?

Answer

All of our systems have been tested with Norton™ Anti-Virus Software.

Answer Id: E12702

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Product FAQ

When I started my run on the Applied Biosystems™ 3130/3130xl Genetic Analyzer, an error occurred: “Leak detected” but I don't see any leaks. What else might cause this?

Answer

“Leak detected” error messages can also occur when the piston on the pump moves further than expected during the movement of the pump. The usual cause for this is a leak on the system, usually due to either an improperly tightened or improperly threaded fitting or a broken capillary. However, if this is not observed, some of the other reasons are:

1. Valve stuck in open position
2. Large air bubble under the piston
3. Polymer:
- Wrong polymer type (e.g., instrument thinks it has POP™-7 on it but it really has POP™-4)
- Old/degraded polymer
4. Improper/no maintenance on the system

Inspect the system for leaks. If you do not see any leaks on the system, perform a water wash using the water wash wizard and place fresh, non-expired polymer and freshly made 1X Running Buffer on the system. If the problem persists, a service call may be required.

Answer Id: E12711

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Product FAQ

How often should I restart my Applied Biosystems™ 3130/3130xl Genetic Analyzer?

Answer

We recommend that you restart the system once a week. The system may require a restart more frequently if you experience issues related to a communication interruption between the instrument and computer. Some systematic issues include:
-The software freezing or becoming unresponsive
-The run timer not counting down or flashing back and forth between two different times
-No data present at the end of the run

In the event of a communication issue between the instrument and computer, close the Data Collection Software (perform a forced shutdown of the application through the Task Manager if Data Collection is unresponsive), completely power down the instrument and turn off the computer. To properly clear the memory, power down the system for at least 2 minutes prior to a restart.

Answer Id: E12691

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Product FAQ

I am only using the Applied Biosystems™ 3130/3130xl Genetic Analyzer once a week. Should I leave the polymer on the instrument or put it back in the refrigerator?

Answer

Putting the polymer back at 2 - 8 degrees C may extend the life of the polymer, however, we do not have any data indicating how long it might extend it. Removing the polymer and putting it back on the system can also result in a trade-off in usage. When placing it back on the system, you will need to degas the polymer for 30 - 60 mins, refill the pump/block, array, and remove bubbles, which will cause you to use more polymer for maintenance than you would for running samples and may decrease the number of runs you can get out of a single polymer bottle/pouch.

Answer Id: E12698

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