What volumes can be used in my plate on the ViiA™ 7 Real-Time PCR System?

Product FAQ

Answer

The following volumes are supported for each instrument block:

- 96-well standard (10-100 μL reactions)
- 96-well Fast (10-30 μL reactions)
- 384-well (5-20 μL reactions)
- TaqMan™ Array Micro Fluidic Cards (approximately 1 μL reactions)

Answer Id: E7191

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Can I collect data at two separate steps for analysis in the ViiA™ 7 Real-Time PCR System?

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Answer

Yes. The ViiA™ 7 system can collect data at multiple steps in the amplification stage. You will need to turn on the data collection for every step of interest. In the analysis, only one set of data can be displayed at a time. To change the data set used for analysis, from your data file go to Analysis Settings then Ct Settings and change the dropdown under ‘Data Step Selection’.

Answer Id: E7192

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Can I see the amplifcation plot on the instrument touchscreen?

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Answer

Yes, on the QuantStudio 12K Flex system you can see the real time amplification plots on the touchscreen. On other systems, you will only be able to see the amplification plots in real-time on the connected computer.

Answer Id: E6321

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What can I use for data analysis on the ViiA™ 7 Real-Time PCR System?

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Answer

For genotyping data, we recommend TaqMan™ Genotyper Software. For relative quantitation, we recommend ExpressionSuite™ Software.

Answer Id: E7193

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What computer configurations can I use with the ViiA™ 7 Fast Real-Time PCR System?

Product FAQ

Answer

For computer and system requirements, please refer to the Release Notes for the ViiA™ 7 Software, which can be found on this page (http://www.thermofisher.com/us/en/home/technical-resources/software-downloads/applied-biosystems-viia-7-real-time-pcr-system.html )(Read Me).

Answer Id: E2328

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How do I set up a custom dye calibration in the ViiA™ 7 or QuantStudio™ 12K Flex systems?

Product FAQ

Answer

Follow the directions on page 10 of this User Bulletin to add a custom dye to your ViiA™ 7 or QuantStudio™ 12K Flex system.

Answer Id: E6317

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What volumes can be used in my plate for the StepOne™ or StepOnePlus™ Real-Time PCR Systems?

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Answer

The StepOne™ and StepOnePlus™ Real-Time PCR Systems have a Fast block, which supports reaction volumes of 10-30 μL.

Answer Id: E7203

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What should I do if the software will not connect to the ViiA™ 7 Real-Time PCR System?

Product FAQ

Answer

After launching the ViiA™ 7 system software, go to the Maintenance Manager. Check if the ViiA™ 7 system icon is present in the “On the Network” window. If not, click on the “Refresh” button to the left of “Remove from My Instruments”.

If you still do not see the ViiA™ 7 icon for the following reasons:
- There is an icon but you cannot add it into the “My Instruments” area
- There is a red “X” on the instrument
- A message says “Disconnected” at the bottom of the window with a red “X” in it then you will need to add the instrument to the “My Instruments” area. Click on the wrench icon in the upper right-hand corner of the icon as pictured below. You may get a message that says that the firmware is older than the one you are trying to run. If appropriate, choose to download the new firmware and let it progress to finish. Do not turn off the instrument during this update. After the firmware update is completed, restart the instrument to check the ViiA™ 7 system icon in ‘On the Network’ area.

Answer Id: E7194

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What does “Error - Analysis failed due to: 1” mean on the StepOne™ or StepOnePlus™ Real-Time PCR System?

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Answer

Please send the file to techsupport@thermofisher.com so that we can have our software group attempt to recover the data.

Answer Id: E7212

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What real-time instruments can be run independently from a computer?

Product FAQ

Answer

The StepOne™, StepOnePlus™, ViiA™ 7, and QuantStudio™ 12K Flex systems can all be run directly from the instrument touchscreen and don't need a separate computer to operate them.

Answer Id: E6303

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What is the normalization calibration for in the ViiA™ 7 or QuantStudio™ 12K Flex systems?

Product FAQ

Answer

The normalization calibration generates factors that the software uses when comparing data from multiple instruments.

Answer Id: E6318

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What type of Real-Time PCR (Sequence Detection System) information is available from Applied Biosystems™?

Product FAQ

Answer

Tutorials demonstrating introductions to Real-Time PCR, guidelines for designing real-time assays using Primer Express™ software, and various instrument set-ups can be found under Life Technologies University on our website. User bulletins, manuals and Product inserts, protocols can be found as downloadable pdf files on our Technical Resources page.

Answer Id: E1359

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How do I load tube strips on the StepOne™ and StepOnePlus™ Real-Time PCR Systems?

Product FAQ

Answer

Follow these instructions for proper loading of tubes, tube strips, and plates:

- Put the tubes or tube strips in the tray with the adapter on the 96-well support base.
- If using a plate, put the plate directly on the 96-well support base.
- Pipet your reactions into the tubes/tube strips or the wells on the plate.
- Seal the tube/tube strips with optical flat caps.
- If using a plate, seal the plate with either optical adhesive film or flat caps.

For optimal performance on the StepOnePlus™ instrument with partial loads:
- Load at least 16 tubes and arrange them in adjacent columns of 8 tubes, using rows A through H, or adjacent rows of 8 tubes, using columns 3 through 10.
For optimal performance on the StepOne™ instrument, load at least 4 tubes in the sample block.

Answer Id: E7204

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What does the error “Calibration file already exists” mean?

Product FAQ

Answer

The calibration files are stored on the ViiA™ 7 instrument; therefore, when you need to perform a new calibration, you will have to delete the old calibration files. From the instrument console, go to the ViiA™ 7 instrument touch screen and select ‘Collect Results’. You should then see a list of files stored on the instrument. Manually select a calibration file such as “Fast-96-cal” and press the ‘Delete’ button to remove the old calibration file. Occasionally, users cannot delete the file off the instrument and may encounter this error: “Some experiments did not collect results therefore cannot be deleted.” For these situations, launch the software, go to ‘maintenance’, manage files, and download the calibration file. The status of the experiment should be displayed as ‘complete’. Next, delete the old calibration file and rerun the calibration.

Answer Id: E7195

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Can I reuse my calibration plates?

Product FAQ

Answer

The calibration plates can be stored and reused three times for up to 1 year after you first open them, so make sure to return them to their original packaging and return them to -20 degrees C storage until the next use. (Note: Due to the small volumes, calibration array cards cannot be reused. Please discard them after use.) If needed, you can make your own background plate using deionized water. Please follow the directions in the ViiA™ 7 Real-Time PCR System Installation, Networking, and Maintenance Guide for more details at http://tools.thermofisher.com/content/sfs/manuals/4442661C.pdf.

Answer Id: E7185

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