Can I reuse my calibration plates for the StepOne™ or StepOnePlus™ Real-Time PCR Systems?

Product FAQ

Answer

The calibration plates can be stored and reused three times for up to one year after you receive them, so make sure to return them to their original packaging and return them to -20 degrees C storage until the next use. If needed, you can make your own background plate using deionized water. Please follow the directions in the StepOne™ and StepOnePlus™ Maintenance and Administration Guide (Appendix C) (http://tools.thermofisher.com/content/sfs/manuals/4376782G.pdf) for more details.

Answer Id: E7202

Was this answer helpful?

Yes
No
Thank you for your response

What volumes can be used in my plate on the ViiA™ 7 Real-Time PCR System?

Product FAQ

Answer

The following volumes are supported for each instrument block:

- 96-well standard (10-100 μL reactions)
- 96-well Fast (10-30 μL reactions)
- 384-well (5-20 μL reactions)
- TaqMan™ Array Micro Fluidic Cards (approximately 1 μL reactions)

Answer Id: E7191

Was this answer helpful?

Yes
No
Thank you for your response

What volumes can be used in my plate for the StepOne™ or StepOnePlus™ Real-Time PCR Systems?

Product FAQ

Answer

The StepOne™ and StepOnePlus™ Real-Time PCR Systems have a Fast block, which supports reaction volumes of 10-30 μL.

Answer Id: E7203

Was this answer helpful?

Yes
No
Thank you for your response

Can I collect data at two separate steps for analysis in the ViiA™ 7 Real-Time PCR System?

Product FAQ

Answer

Yes. The ViiA™ 7 system can collect data at multiple steps in the amplification stage. You will need to turn on the data collection for every step of interest. In the analysis, only one set of data can be displayed at a time. To change the data set used for analysis, from your data file go to Analysis Settings then Ct Settings and change the dropdown under ‘Data Step Selection’.

Answer Id: E7192

Was this answer helpful?

Yes
No
Thank you for your response

What can I do when I get the error - “Array index out of range: 1” in the ViiA™ 7 Real-Time PCR System?

Product FAQ

Answer

This error can occur when the run was started from a corrupt template file or if the file name is very long or has illegal characters. Delete any templates that were created in v1.1 or earlier. Upgrade your software to the most current version. Recreate your templates in v1.2 or later. Only use templates created in v1.2 or later for any future runs. If the file name is very long, try using the “Save As” feature to resave the file with a shorter name. If this does not fix the problem, please send your file to techsupport@thermofisher.com for further assistance.

Answer Id: E7198

Was this answer helpful?

Yes
No
Thank you for your response

What is the normalization calibration for in the ViiA™ 7 or QuantStudio™ 12K Flex systems?

Product FAQ

Answer

The normalization calibration generates factors that the software uses when comparing data from multiple instruments.

Answer Id: E6318

Was this answer helpful?

Yes
No
Thank you for your response

How do I load tube strips on the StepOne™ and StepOnePlus™ Real-Time PCR Systems?

Product FAQ

Answer

Follow these instructions for proper loading of tubes, tube strips, and plates:

- Put the tubes or tube strips in the tray with the adapter on the 96-well support base.
- If using a plate, put the plate directly on the 96-well support base.
- Pipet your reactions into the tubes/tube strips or the wells on the plate.
- Seal the tube/tube strips with optical flat caps.
- If using a plate, seal the plate with either optical adhesive film or flat caps.

For optimal performance on the StepOnePlus™ instrument with partial loads:
- Load at least 16 tubes and arrange them in adjacent columns of 8 tubes, using rows A through H, or adjacent rows of 8 tubes, using columns 3 through 10.
For optimal performance on the StepOne™ instrument, load at least 4 tubes in the sample block.

Answer Id: E7204

Was this answer helpful?

Yes
No
Thank you for your response

Can I see the amplifcation plot on the instrument touchscreen?

Product FAQ

Answer

Yes, on the QuantStudio 12K Flex system you can see the real time amplification plots on the touchscreen. On other systems, you will only be able to see the amplification plots in real-time on the connected computer.

Answer Id: E6321

Was this answer helpful?

Yes
No
Thank you for your response

What can I use for data analysis on the ViiA™ 7 Real-Time PCR System?

Product FAQ

Answer

For genotyping data, we recommend TaqMan™ Genotyper Software. For relative quantitation, we recommend ExpressionSuite™ Software.

Answer Id: E7193

Was this answer helpful?

Yes
No
Thank you for your response

How do I activate or renew my software license for the ViiA™ 7 Real-Time PCR System?

Product FAQ

Answer

In the ViiA™ 7 Real-Time PCR System, please follow the instructions found in this User Bulletin: How to Activate or Renew a License (http://tools.thermofisher.com/content/sfs/manuals/4474347B.pdf). If you have a license key associated with computer that is no longer in use, please contact our Instrument Services group at instrumentservices@lifetech.com with the serial number and license key so that they can reassign it for you.

Answer Id: E7199

Was this answer helpful?

Yes
No
Thank you for your response

What should I do if I need to transfer my ViiA™ 7 system software license to another computer?

Product FAQ

Answer

If you need to move your ViiA™ 7 system software to another computer, please contact instrumentservices@lifetech.com with your instrument serial number.

Answer Id: E6319

Was this answer helpful?

Yes
No
Thank you for your response

What computer configurations can I use with the ViiA™ 7 Fast Real-Time PCR System?

Product FAQ

Answer

For computer and system requirements, please refer to the Release Notes for the ViiA™ 7 Software, which can be found on this page (http://www.thermofisher.com/us/en/home/technical-resources/software-downloads/applied-biosystems-viia-7-real-time-pcr-system.html )(Read Me).

Answer Id: E2328

Was this answer helpful?

Yes
No
Thank you for your response

What plates or tubes can I use with the StepOne™ and StepOnePlus™ Real-Time PCR Systems?

Product FAQ

Answer

Please refer to this selection table (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/real-time-pcr-plastics.html) for compatible plates, tubes, and films.

Answer Id: E7205

Was this answer helpful?

Yes
No
Thank you for your response

Can I import a standard curve for my real-time data?

Product FAQ

Answer

It is possible to import a standard curve if you have either the ViiA™ 7 or QuantStudio™ 12K Flex systems. In the ViiA™ 7 or QuantStudio&trade instrument software, open the data file and go to 'Analysis Settings'. Click on the 'Standard Curve Settings' tab. You will see an option to 'Use a standard curve imported from another experiment'. Choose this, then click on "Import". Browse to the proper data file and click "Select".

Answer Id: E6310

Was this answer helpful?

Yes
No
Thank you for your response

When do I need to calibrate the ViiA™ 7 Real-Time PCR System?

Product FAQ

Answer

The recommended maintenance schedule and calibration instructions can be found in the User Guide here: http://tools.thermofisher.com/content/sfs/manuals/4442661C.pdf
In brief, the background calibration is recommended to be done every month. All other calibrations (pure dye, ROI, uniformity, and normalization) are recommended to be done every six months.

Answer Id: E7183

Was this answer helpful?

Yes
No
Thank you for your response