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Product FAQ

What should I do if I need to transfer my ViiA™ 7 system software license to another computer?

Answer

If you need to move your ViiA™ 7 system software to another computer, please contact instrumentservices@lifetech.com with your instrument serial number.

Answer Id: E6319

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Product FAQ

How do I set up a touchdown PCR on the StepOne™ and StepOnePlus™ Real-Time PCR Systems?

Answer

In a touchdown PCR experiment, you will change either the temperature or the time of a particular PCR step with every cycle. Most commonly, the annealing temperature is adjusted throughout the experiment, such that the specificity is higher in the early cycles and the efficiency in the later cycles.

1. Go to File then New Experiment then Advanced Setup. Fill out the relevant options as you normally would.
2. Go to the Run Method under the ‘Setup’ section and you should see the Graphical View of your thermal profile. Check the box next to ‘Enable AutoDelta’. You should see some grey triangles appear next to the Temperature and Time at every step in the Cycling Stage. (Note: If you want to start the changes at a later cycle, set this here under ‘Starting Cycles’.)
3. A new window called ‘AutoDelta Settings’ will open up. Select the appropriate options and click ‘Save Setting’. You will then see a green triangle show up next to the parameter you changed. Your new method has now been applied.

Answer Id: E7207

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Product FAQ

What qPCR instruments are compatible with Applied Biosystems™ SymphoniqPCR™ Analysis Software?

Answer

Applied Biosystems™ QuantStudio™ 12K Flex, Applied Biosystems™ QuantStudio™ 6 and 7, Applied Biosystems™ ViiA™ 7, 7500 Fast (eds files only), 7500, 7900 HT, and Applied Biosystems™ StepOne™/ StepOnePlus ™ real-time PCR systems are compatible with SymphoniqPCR Analysis Software.

Answer Id: E10235

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Product FAQ

What concentration of ROX™ should I use for the ViiA™ 7 system?

Answer

If using a mastermix in which ROX™ dye is provided in a separate tube, please add it to 50 nM (in the final reaction well).

Answer Id: E6320

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Product FAQ

Can I import a standard curve for my real-time data?

Answer

It is possible to import a standard curve if you have either the ViiA™ 7 or QuantStudio™ 12K Flex systems. In the ViiA™ 7 or QuantStudio&trade instrument software, open the data file and go to 'Analysis Settings'. Click on the 'Standard Curve Settings' tab. You will see an option to 'Use a standard curve imported from another experiment'. Choose this, then click on "Import". Browse to the proper data file and click "Select".

Answer Id: E6310

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Product FAQ

Where are my files saved to on the StepOne™ and StepOnePlus™ Real-Time PCR Systems?

Answer

Run files will be saved to both the instrument and the connected computer. On the connected computer, files will be saved to the default data folder, unless you change it.

To find or change the default folder, go to Tools then Preferences then Defaults. Here you will see a Data Folder and an Import Folder. The default location is shown. If you want files to be saved to (or open from) a different location, click ‘Browse’ and choose the new folder.

Answer Id: E7208

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Product FAQ

What does “Error - Analysis failed due to: 1” mean on the StepOne™ or StepOnePlus™ Real-Time PCR System?

Answer

Please send the file to techsupport@thermofisher.com so that we can have our software group attempt to recover the data.

Answer Id: E7212

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Product FAQ

Can I see the amplifcation plot on the instrument touchscreen?

Answer

Yes, on the QuantStudio 12K Flex system you can see the real time amplification plots on the touchscreen. On other systems, you will only be able to see the amplification plots in real-time on the connected computer.

Answer Id: E6321

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Product FAQ

What does “Error - Java.lang.IllegalArgumentException: One of the raw spectra is null” mean?

Answer

This issue can occur if the “Start Run” button is clicked more than once or if illegal characters are used in the experiment/file name. You can recover the data yourself by overriding the calibration file with one that represents the calibration status of the instrument when you ran that particular file. Use the toolbar at the top to navigate: Analysis then Override Calibration then Use Calibration From Another File.

Answer Id: E7215

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Product FAQ

Can I use a custom dye on my Applied Biosystems™ 7500, 7500 Fast, 7900HT Fast or other Real-Time PCR System?

Answer

Yes, you can run custom dyes, but the custom dye must fall into the wavelengths that are capable of being detected by a specific instrument. For optimal results on the filter-based instruments (including 7500 and 7500 Fast), the custom dye emission wavelength must fall into one of the emission filter peak detection wavelengths. For the laser-based 7900HT systems, the custom dye wavelength must fall within the overall range of detection for the instrument. Please refer to the Real-Time PCR information pages on our website under “Products & Services” for profiles of each instrument with a list of compatible standard dyes and the corresponding wavelengths supported. Please also refer to your User Manual or Installation and Maintenance Manual for instructions on how to calibrate custom dyes on your particular instrument.

Answer Id: E2457

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Product FAQ

What dyes can I use with the ViiA™ 7 Real-Time PCR System?

Answer

The ViiA™ 7 is calibrated for the following dyes: FAM™, VIC™, ROX™, SYBR™, NED, and TAMRA. In addition, it can be used with custom dyes that excite between 455 and 672 nm and read between 505 and 723 nm.

Answer Id: E6305

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Product FAQ

What plates or tubes can I use with the StepOne™ and StepOnePlus™ Real-Time PCR Systems?

Answer

Please refer to this selection table (https://www.thermofisher.com/us/en/home/life-science/pcr/pcr-plastics/real-time-pcr-plastics.html) for compatible plates, tubes, and films.

Answer Id: E7205

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Product FAQ

How do I know if my software license has expired?

Answer

You can check your license status by opening the instrument software and going to Tools --> License Central. You will see the license status and the expiration date.

Answer Id: E6626

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Product FAQ

What does “Error - Failure to acquire needed controlling rights” mean?

Answer

This error usually occurs when you first start your run. Reboot the instrument and restart the computer. Make sure to log into the computer as the Administrator (on the Windows™ OS).

Answer Id: E7210

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Product FAQ

What does “Error - Analysis failed due to GExSession doesn’t exist in context” mean?

Answer

This error means that the data collection was not turned on as appropriate. If this is inadvertently turned off, no data will be collected. Thus, the data are not retrievable in such a file.

Answer Id: E7213

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