When do I need to calibrate the ViiA™ 7 Real-Time PCR System?

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Answer

The recommended maintenance schedule and calibration instructions can be found in the User Guide here: http://tools.thermofisher.com/content/sfs/manuals/4442661C.pdf
In brief, the background calibration is recommended to be done every month. All other calibrations (pure dye, ROI, uniformity, and normalization) are recommended to be done every six months.

Answer Id: E7183

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What are the uniformity and normalization calibrations for on the ViiA™ 7 Real-Time PCR System?

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Answer

The uniformity calibration generates data that allow the software to compensate for the physical effects of the ViiA™ 7 system filters using the ROI plate. The normalization calibration generates factors that the software uses when comparing data from multiple ViiA™ 7 instruments within a study. Two normalization plates are provided in the calibration kit, one containing FAM™/ROX™ dyes and the other with VIC™/ROX™ dyes.

Answer Id: E7184

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What volumes can be used in my plate on the ViiA™ 7 Real-Time PCR System?

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Answer

The following volumes are supported for each instrument block:

- 96-well standard (10-100 μL reactions)
- 96-well Fast (10-30 μL reactions)
- 384-well (5-20 μL reactions)
- TaqMan™ Array Micro Fluidic Cards (approximately 1 μL reactions)

Answer Id: E7191

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Can I reuse my calibration plates?

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The calibration plates can be stored and reused three times for up to 1 year after you first open them, so make sure to return them to their original packaging and return them to -20 degrees C storage until the next use. (Note: Due to the small volumes, calibration array cards cannot be reused. Please discard them after use.) If needed, you can make your own background plate using deionized water. Please follow the directions in the ViiA™ 7 Real-Time PCR System Installation, Networking, and Maintenance Guide for more details at http://tools.thermofisher.com/content/sfs/manuals/4442661C.pdf.

Answer Id: E7185

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Can I collect data at two separate steps for analysis in the ViiA™ 7 Real-Time PCR System?

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Answer

Yes. The ViiA™ 7 system can collect data at multiple steps in the amplification stage. You will need to turn on the data collection for every step of interest. In the analysis, only one set of data can be displayed at a time. To change the data set used for analysis, from your data file go to Analysis Settings then Ct Settings and change the dropdown under ‘Data Step Selection’.

Answer Id: E7192

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Can I reuse my calibration plates for the StepOne™ or StepOnePlus™ Real-Time PCR Systems?

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Answer

The calibration plates can be stored and reused three times for up to one year after you receive them, so make sure to return them to their original packaging and return them to -20 degrees C storage until the next use. If needed, you can make your own background plate using deionized water. Please follow the directions in the StepOne™ and StepOnePlus™ Maintenance and Administration Guide (Appendix C) (http://tools.thermofisher.com/content/sfs/manuals/4376782G.pdf) for more details.

Answer Id: E7202

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How do I activate or renew my software license for the ViiA™ 7 Real-Time PCR System?

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Answer

In the ViiA™ 7 Real-Time PCR System, please follow the instructions found in this User Bulletin: How to Activate or Renew a License (http://tools.thermofisher.com/content/sfs/manuals/4474347B.pdf). If you have a license key associated with computer that is no longer in use, please contact our Instrument Services group at instrumentservices@lifetech.com with the serial number and license key so that they can reassign it for you.

Answer Id: E7199

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What can I use for data analysis on the ViiA™ 7 Real-Time PCR System?

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Answer

For genotyping data, we recommend TaqMan™ Genotyper Software. For relative quantitation, we recommend ExpressionSuite™ Software.

Answer Id: E7193

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What volumes can be used in my plate for the StepOne™ or StepOnePlus™ Real-Time PCR Systems?

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Answer

The StepOne™ and StepOnePlus™ Real-Time PCR Systems have a Fast block, which supports reaction volumes of 10-30 μL.

Answer Id: E7203

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When do I need to calibrate the StepOne™ or StepOnePlus™ Real-Time PCR System?

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Answer

Recommended Maintenance Schedule

Power on/off the computer controlling the instrument: Weekly
Check computer disk space. If necessary, archive or back up your experiment files and instrument settings: Weekly
Background calibration: Every month
Run disk cleanup and disk defragmentation: Every month
Perform an instrument self test: Every month
Pure dye calibrations: Every 18 months
Spatial calibration: Every 18 months
RNaseP instrument verification: After the instrument has been moved, or as needed to verify instrument performance

Answer Id: E7200

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What qPCR instruments are compatible with Applied Biosystems™ SymphoniqPCR™ Analysis Software?

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Answer

Applied Biosystems™ QuantStudio™ 12K Flex, Applied Biosystems™ QuantStudio™ 6 and 7, Applied Biosystems™ ViiA™ 7, 7500 Fast (eds files only), 7500, 7900 HT, and Applied Biosystems™ StepOne™/ StepOnePlus ™ real-time PCR systems are compatible with SymphoniqPCR Analysis Software.

Answer Id: E10235

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What should I do if I need to transfer my ViiA™ 7 system software license to another computer?

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Answer

If you need to move your ViiA™ 7 system software to another computer, please contact instrumentservices@lifetech.com with your instrument serial number.

Answer Id: E6319

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What does “Error - Fatal Error Number 0x8158” mean?

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Answer

This is generally caused by incorrect placement of tube strips, resulting in a sample block that can’t properly be lowered. We recommend contacting Instrument Services to have an engineer walk you through a manual drawer-lowering process during instrument power-up. To prevent this from happening in the future, place tubes toward the middle of the block or use a placeholder strip on the opposing side to counterbalance block pressure.

Answer Id: E7216

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Can I see the amplifcation plot on the instrument touchscreen?

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Answer

Yes, on the QuantStudio 12K Flex system you can see the real time amplification plots on the touchscreen. On other systems, you will only be able to see the amplification plots in real-time on the connected computer.

Answer Id: E6321

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How do I renew my software license?

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Answer

User Bulletin v1.2.1 has specific directions for how to renew your software license. Please search the term '4474347' on our website. The renewal directions apply to the ViiA™ 7, QuantStudio™ 6, QuantStudio™ 7, and QuantStudio™ 12K Flex systems.

Answer Id: E6627

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