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These Product FAQs pertain to RUO products. These products are for Research Use Only. Not for use in diagnostic procedures. If using an IVD product, please refer to your specific product information.
How do I remove DNase from an RNA sample?
DNase can be removed by the following methods: acid phenol:choloform extraction, lithium chloride precipitation, EDTA, and heat inactivation, or using our MegaClear™ Transcription Clean-Up Kit (Cat. No. AM1908).
Alternatively, our Ambion™ TURBO DNA-free™ Kit (Cat. No. AM1907) is supplied with a DNase inactivation reagent that can be used to remove the DNase, as well as divalent cations such as magnesium and calcium, which can catalyze RNA degradation when RNA is heated with the sample.
Answer Id: E7822
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