How do I remove DNase from an RNA sample?

Product FAQ

Answer

DNase can be removed by the following methods: acid phenol:choloform extraction, lithium chloride precipitation, EDTA, and heat inactivation, or using our MegaClear™ Transcription Clean-Up Kit (Cat. No. AM1908).

Alternatively, our Ambion™ TURBO DNA-free™ Kit (Cat. No. AM1907) is supplied with a DNase inactivation reagent that can be used to remove the DNase, as well as divalent cations such as magnesium and calcium, which can catalyze RNA degradation when RNA is heated with the sample.

Answer Id: E7822

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What is the binding capacity of the MEGAclear™ kit filter column

Product FAQ

Answer

The binding capacity of the 10051G filter columns is 500µg of RNA

Answer Id: E2866

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